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Measuring method for algae biomass in biological soil crust

A biological soil and measurement method technology, applied to the determination of algae crust biomass and the field of algae biomass measurement in biological soil crusts, can solve the problem of inability to compare the relationship between algae crusts and vascular plants, and the inability to accurately evaluate algae crusts. The skin biomass contribution rate and other issues can be easily realized in the cultivation environment, weight interference can be eliminated, and the results can be accurate.

Active Publication Date: 2015-11-25
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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Problems solved by technology

However, it is difficult to directly weigh the biomass of biological soil crusts because the crusts and soil are tightly cemented together. Therefore, it is impossible to compare the relationship between algal crusts and vascular plant biomass, and it is also impossible to accurately assess the biological value of algal crusts. Contribution rate of quantity to ecosystem productivity

Method used

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  • Measuring method for algae biomass in biological soil crust

Examples

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example 1

[0023] Example 1: Determination of Algal Crust Biomass Developed in Interdune Lowlands

[0024] In August 2014, in the artificial vegetation sand fixation area established in 1990 at the Shapotou National Station of the Chinese Academy of Sciences in Zhongwei City, Ningxia Hui Autonomous Region, 10 samples of well-developed algae crusts were collected from the lowland between dunes, each with an area of ​​9cm 2 . Sampling uses a self-made 3cm*3cm*0.5cm square stainless steel frame; specific methods, such as: algae crust sample sampling, sieving, cleaning, algae culture, determination of chlorophyll a and b, standard curve drawing and natural growth of algal organisms The determination of the quantity is described as follows:

[0025] a. When sampling, first press the sampling frame firmly into the soil until the sampling frame completely enters the soil, then gently take out about 50cm inside the stainless steel frame with a shovel sterilized with 75% alcohol 2 Algae crusts,...

example 2

[0032] Example 2: Determination of Algal Crust Biomass Developed on Sand Dune Tops

[0033] In August 2014, in the artificial vegetation sand-fixation area established in 1990 at the Shapotou National Station of the Chinese Academy of Sciences in Zhongwei City, Ningxia Hui Autonomous Region, 10 samples of well-developed algal crusts were collected from the lowlands on the top of the dunes, and the area of ​​each sample was 9cm2. A self-made square stainless steel frame of 3cm*3cm*0.5cm was used for sampling; specific methods, such as sampling, sieving, cleaning, and culturing of algae crust samples were the same as in Example 1.

[0034] Determination of chlorophyll a and b: Chlorophyll is extracted by the mixed solution method (extract solution: absolute ethanol: acetone: water = 4.5:4.5:1). Put the collected algae crust sample into a test tube filled with 10mL extract solution, plug it with a rubber stopper, and repeat each treatment 3 times; put it in a thermostat at 45°C a...

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Abstract

The invention discloses a measuring method for the algae biomass in a biological soil crust, and relates to the field of biology. The method includes the steps of sampling of an algae crust sample, sieving, cleaning, algae cultivating, chlorophyll a and chlorophyll b measuring, standard curve drawing and algae biomass measuring (natural development). In the algae cultivating step, cultivating is carried out through BG11 cultivating media, and use algae are mixed algae. In the chlorophyll a and chlorophyll b measuring step, chlorophyll is extracted with a mixed solution method, wherein extracting solutions comprise absolute ethyl alcohol, acetone and water in the ratio of 4.5 to 4.5 to 1. The relationship between the gross of the chlorophyll a and the chlorophyll b of the algae and the algae biomass is regressed through a standard curve. Measuring of the natural developing algae biomass is converted through the standard curve between the gross of the chlorophyll a and the chlorophyll b and the cultivated algae biomass. Direct comparison between the algae crust and the vascular plant biomass is achieved, and a reliable approach is provided for more accurately calculating the productivity of the algae crust and the effect of the productivity in an ecological system.

Description

technical field [0001] The invention relates to a method for measuring the biomass of algae in biological soil crusts, which is suitable for measuring the biomass of algae crusts, and aims at realizing the comparison between the biomass of algae crusts and vascular plants, belonging to the field of biology. Background technique [0002] Biological soil crusts are the main builders and ecosystem engineers of desert ecosystems, and are an important feature of desert ecosystem composition and surface landscape. They are tiny particles on the soil surface that are surrounded by bacteria, microscopic fungi, cyanobacteria, diatoms or green algae, lichens and bryophytes that live in the shallow soil (or sometimes live on the soil surface), even up to a few millimeters above the soil surface, And the common non-vascular plant components in many landscapes use mycelium, rhizoids and exudates to "cement" to form complex and relatively stable aggregate structures with a thickness of 3-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 赵洋李新荣贾荣亮
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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