The invention discloses a soft-shelled turtle feed capable of enhancing disease resistance and a preparation method thereof. The feed disclosed by the invention is prepared form the following raw materials in parts by weight: 35-45 parts of white fish meal, 15-20 parts of chicken blood meal, 12-18 parts of corn flour, 5-10 parts of soybean straw powder, 4-7 parts of monosodium glutamate protein, 3-5 parts of sleeve-fish visceral meal, 2-3 parts of rhizoma zingiberis powder, 1.5-2.5 parts of soya bean lecithin, 2-3 parts of aloe dry powder, 3.5-4.5 parts of bamboo insect meal, 1-2 parts of angelica keiskei, 2-3 parts of houttuynia cordata, 1.5-2.5 parts of grape skins, 1-2 parts of schizandra fruit, 2-3 parts of codonopsis pilosula, 1-2 parts of dandelion, 2-3 parts of Echinacea and 1-2 parts of a premix. The feed disclosed by the invention is rich in nutrition, high in palatability and good in digestive absorption property, is capable of improving the appetite of soft-shelled turtles, ensuring the high growth speed after being eaten by the soft-shelled turtles, improving the immunologic function of the soft-shelled turtles, enhancing the disease resistance of the soft-shelled turtles, increasing the survival rate of the soft-shelled turtles and reducing the breeding cost, is free from the need of adding antibiotic medicines, enables the meat quality of the soft-shelled turtles to be approximate to that of wide soft-shelled turtles, is free from medicine residue and pollution and is safe, and the feed coefficient is reduced.