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Method for preparing antioxidant active peptide by hydrolyzing fish scale collagen with co-immobilized double enzymes

A technology of fish scale collagen and antioxidant active peptide is applied in the preparation method of peptide, chemical instrument and method, fixed on/in organic carrier, etc., to achieve non-toxic side effect, easy purification and high enzymatic hydrolysis efficiency. Effect

Active Publication Date: 2012-07-04
瑞金市工业投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing literature, there is no relevant report on the processing of aquatic products using multi-enzyme co-immobilization technology in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Dissolve papain and Alcalase alkaline protease in phosphate buffer at a ratio of 1:1 to prepare a protease solution with a mass concentration of 1.0%; meanwhile, prepare a pH 7.0 mass concentration of 2.0% alginic acid Sodium solution, take the prepared protease solution and add it to the sodium alginate solution. The volume ratio of the protease solution to the sodium alginate solution is 1:1. After fully stirring, add 0.5% CaCl 2 In the solution, transfer it to a 4℃ refrigerator to harden for 6 h, filter and wash. Then add 0.1% glutaraldehyde solution in volume fraction, shake for 0.5 h, let stand for 4 h at 25℃, wash away residual glutaraldehyde and unimmobilized enzyme with phosphate buffer, and blow dry with cold air Surface water has to co-immobilize the dual enzyme.

[0029] (2) Using fresh grass carp fish scales as raw materials, soak and wash the surface adhesions of the fish scales, dry at 40°C, then crush the washed and dried fish scales into fine powder, a...

Embodiment 2

[0038] (1) Dissolve the neutral protease and Alcalase alkaline protease in the phosphate buffer at a ratio of 1:2 to prepare a protease solution with a mass concentration of 3.0%; meanwhile, prepare a pH 9.0 mass concentration of 4.0% seaweed Sodium solution, add the prepared protease solution to the sodium alginate solution, the volume ratio of the protease solution to the sodium alginate solution is 1:3. After stirring well, add CaCl with a mass concentration of 2.5% 2 In the solution, transfer to a 4℃ refrigerator to harden for 4 h, filter and wash. Then add 1.5% glutaraldehyde solution with volume fraction, shake for 0.5 h, stand for 5 h at 15℃ for cross-linking, wash away residual glutaraldehyde and unimmobilized enzyme with phosphate buffer, and blow dry with cold air The surface moisture has to be co-immobilized dual enzymes.

[0039] (2) Using fresh grass carp fish scales as raw materials, wash the surface adhesions of the fish scales by immersion, dry at 40°C, then crus...

Embodiment 3

[0048] (1) Dissolve the neutral protease and papain in the phosphate buffer in the ratio of activity ratio of 1:3, prepare a protease solution with a mass concentration of 5.0%; meanwhile, prepare a pH 6.0 with a mass concentration of 6.0% sodium alginate Solution: Take the prepared protease solution and add it to the sodium alginate solution. The volume ratio of the protease solution to the sodium alginate solution is 1:5. After stirring well, add CaCl with mass concentration of 5.0% 2 In the solution, transfer to a 4℃ refrigerator to harden for 2 hours, filter and wash. Then add the volume fraction of 3.0% glutaraldehyde solution, shake for 0.5 h, let stand for 6 h at 4℃, wash away residual glutaraldehyde and unimmobilized enzyme with phosphate buffer, and blow dry with cold air The surface moisture has to be co-immobilized dual enzymes.

[0049] (2) Using fresh grass carp fish scales as raw materials, wash the surface adhesions of the fish scales by immersion, dry at 40°C, th...

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Abstract

The invention discloses a method for preparing an antioxidant active peptide by hydrolyzing fish scale collagen with co-immobilized double enzymes. The method comprises the following steps of: (1) co-immobilizing double enzymes by cross-linking method, (2) pre-treating fish scale, (3) hydrolyzing fish scale proteins with the co-immobilized double enzymes, (4) decolorizing and deodorizing, (5) separating fish scale polypeptides by ultrafiltration, (6) vacuum concentrating; and (7) freeze drying. The antioxidant active peptide product can be used as a natural antioxidant in antioxidation and preservation of foods and has no toxic or side effect and high safety. The method has high enzymolysis efficiency, the enzymes can be used repeatedly, and the enzymatic hydrolysates are purified easily.The method provides a new approach to effective utilization of fresh water fish waste, and the product can be used in pharmaceuticals, foods and cosmetics.

Description

Technical field [0001] The invention belongs to the field of food biotechnology and relates to a method for preparing biologically active peptides. Background technique [0002] my country is rich in fish resources, and the amount of freshwater fish farming ranks first in the world. At present, the processing of freshwater fish is mainly limited to the processing of fish body muscles, such as surimi and fish balls. Restricted by traditional concepts and processing technology, a large amount of leftovers such as fish scales and offal produced in the process of fish meat processing are rarely used, but thrown away as garbage. It is estimated that 300,000 tons of fish scales are discarded every year in my country, which not only wastes resources, but also pollutes the environment. [0003] Fish scales are rich in protein, accounting for 50%-70% of the total mass of fish scales, mainly collagen, followed by keratin and other mucins. Studies have found that the collagen in fish scale...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12N11/10C07K1/34
Inventor 杨安树陈红兵吴志华李欣
Owner 瑞金市工业投资发展有限公司
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