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Efficient enzyme hydrolysis method of lignocellulose biomass

A lignocellulosic and lignocellulose technology, which is applied in the field of lignocellulose hydrolysis and high-efficiency enzymatic hydrolysis of lignocellulosic biomass, to achieve the effects of increasing the concentration of enzymatic hydrolysis raw materials, high hydrolysis efficiency, and high sugar production concentration

Inactive Publication Date: 2011-09-07
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent (3) is related to the present invention to a certain extent, but its focus is to propose a new pretreatment process, that is, a two-step segmental temperature-variable high-temperature liquid water hydrolysis process, which does not involve subsequent enzymatic hydrolysis

Method used

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  • Efficient enzyme hydrolysis method of lignocellulose biomass

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Experimental program
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Effect test

Embodiment 1

[0033] Crush the sugar-free sweet sorghum stalk residue after squeezing to 40 mesh, and add it to the high-temperature liquid water reaction kettle according to the ratio of solid-liquid ratio of 1:10g / mL. , processing for 15 minutes, completing pretreatment, solid-liquid separation, and obtaining hemicellulose hydrolyzate and pretreated sweet sorghum stalk residue substrate. Wash with clean water to remove enzyme hydrolysis and fermentation inhibitors, and use it as raw material for enzyme hydrolysis. Take 1 g of sweet sorghum stalk substrate as raw material for enzymatic hydrolysis, add 10 mL of 0.05 M acetic acid-sodium acetate buffer solution (pH 4.5), add cellulase at a ratio of 10 FPU / g cellulose or dextran, and add 0.075 mL Tween80, stirred and hydrolyzed at 45°C for 12 hours, then added 0.5 g of sweet sorghum stalk substrate, added cellulase at a ratio of 10 FPU / g dextran, and added 0.0375 mL Tween80 at the same time, stirred and hydrolyzed for 36 hours, then added Ad...

Embodiment 2

[0035] Crush the sweet sorghum stalk residue after squeezing to 60 mesh, add it to the high-temperature liquid water reactor at a solid-to-liquid ratio of 1:20 g / mL, and treat it for 30 minutes in a high-temperature liquid water environment of 4Mpa, 190°C, and 500rpm to complete Pretreatment, solid-liquid separation, obtain hemicellulose hydrolyzate and pretreated sweet sorghum stalk substrate, wash with water, remove enzyme hydrolysis and fermentation inhibitors, and use it as raw material for enzyme hydrolysis. Take 1.5g as raw material for enzymatic hydrolysis, add 10mL 0.1M acetic acid-sodium acetate buffer (pH4.8), add cellulase at a ratio of 20FPU / g dextran, and add 0.3mL Tween80 at the same time, magnetically After stirring and hydrolyzing for 24 hours, add 0.75g of substrate, add cellulase at the ratio of 20FPU / g dextran, and add 0.15mL Tween80 at the same time, after magnetic stirring and hydrolyzing for 48h, then add 0.75g of pretreatment material, and then add 0.75g ...

Embodiment 3

[0037] The sweet sorghum stalk residue after squeezing the juice was crushed to 60 mesh, and added to the high-temperature liquid water reaction kettle according to the solid-liquid ratio of 1:20g / mL, and treated in the high-temperature liquid water environment of 4Mpa, 180°C, and 400rpm for 20min, and the pre-treatment was completed. treatment and solid-liquid separation to obtain hemicellulose hydrolyzate and pretreated sweet sorghum stalk substrate, washed with water to remove enzyme hydrolysis and fermentation inhibitors, and used as raw materials for enzyme hydrolysis. Take 1g as raw material for enzymatic hydrolysis, add 10mL 0.05M acetic acid-sodium acetate buffer (pH4.8), add cellulase at a ratio of 20FPU / g dextran, and add 0.075mL / g Tween80 at the same time, at 50℃ After 24 hours of magnetic stirring and hydrolysis, add 0.5g of pretreatment material, add cellulase at a ratio of 20FPU / g dextran, and add 0.0375mL Tween80 at the same time, after magnetic stirring and hydr...

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Abstract

The invention discloses an efficient enzyme hydrolysis method of lignocellulose biomass, comprising the following steps: pretreating the lignocellulose biomass through high-temperature aqueous water to obtain a lignocellulose raw material to be used as a substrate; dispersing the substrate into an acetic acid-sodium acetate buffer solution in a certain concentration; adding a nonionic surfactant and cellulose; stirring the materials; hydrolyzing for some time; adding a certain amount of materials twice; and simultaneously adding the nonionic surfactant and the cellulose; and continuously hydrolyzing to obtain a saccharide compound and lignocellulose residues. In the method provided by the invention, high-temperature aqueous water pretreatment, batch charging, nonionic surfactant adding and stirring and an enzyme hydrolysis process are coupled, thus the hydrolysis efficiency is high, the concentration of the enzymolysized lignocellulose raw material can be greatly improved; the concentration reaches 30%; the sugar generation concentration and the hydrolysis efficiency are high under the condition of high-concentration lignocellulose raw materials; the enzymolysized raw material concentration is improved; and simultaneously the high-concentration sugar is produced, thus improving the hydrolysis efficiency, and realizing the efficient enzyme hydrolysis of the lignocellulose biomass.

Description

Technical field: [0001] The invention belongs to the lignocellulose hydrolysis technology in the field of biochemistry and chemical industry, in particular to a method for high-efficiency enzymatic hydrolysis of lignocellulosic biomass. Background technique: [0002] With the high development of society, the consumption of fossil fuels is increasing. Due to the non-renewability of fossil fuels, the search for alternative energy has become the focus of attention. Fuel ethanol due to its high octane number, clean and low CO 2 Emissions are favored. The traditional raw materials for preparing ethanol come from grain and sugar crops such as corn, wheat, and sugarcane. The production technology is mature, but there is a problem of "competing with people for food and with farmers for land". Cellulosic ethanol is produced from lignocellulosic biomass such as corn stalks, corn cobs, rice stalks and other crop stalks, wood chips, branches and other agricultural and forestry wastes....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCY02E50/16Y02E50/10
Inventor 庄新姝袁振宏王闻马隆龙亓伟谭雪松王琼许敬亮徐惠娟
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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