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Bagasse pretreatment method

A sugarcane bagasse and pretreatment technology, applied in fermentation and other directions, can solve problems such as waste of resources, high content of fermentation inhibitors, pollution, etc., and achieve the effects of reducing pretreatment costs, high reducing sugar yield, and high enzymatic hydrolysis efficiency.

Inactive Publication Date: 2013-07-17
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still technical bottlenecks such as high cost, low efficiency, and serious pollution that need to be broken through. Further research is needed to reduce pretreatment costs and improve pretreatment efficiency.
[0006] At present, most of the literature reports about bagasse NaOH or H 2 o 2 Pretreatment is processed under the action of a separate solution, in which H 2 o 2 The concentration of the solution is relatively high, especially the NaOH pretreatment is mostly carried out under high temperature and high pressure conditions, and the equipment cost is high; it will cause a large part of the cellulose to be removed, wasting resources; the content of the fermentation inhibitors produced is large; the optimized combination of NaOH and H 2 o 2 There are few studies on bagasse pretreatment of mixed solutions

Method used

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Examples

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Effect test

Embodiment 1

[0036] Embodiment 1: the pretreatment method of bagasse, concrete operations are as follows:

[0037] (1) After drying 40g of bagasse at 80°C for 48 hours, crushing and sieving to obtain raw materials with a particle size of 18-40 mesh;

[0038] (2) Add bagasse to NaOH and H 2 o 2 In the mixed aqueous solution, at 42°C, 150rpm shaker pretreatment for 24h, wherein the final concentration of NaOH in the mixed aqueous solution is 1.0%, H 2 o 2 The final concentration in the mixed aqueous solution is 1.0%, and the mass volume (w / v) ratio of the mixed aqueous solution to bagasse is 15:1;

[0039] (3) Filter the pretreated bagasse, wash the filter residue with distilled water at 65°C until the filtrate is 7.0, and dry at 60°C to constant weight to obtain the pretreated bagasse for later use.

[0040] Put the dried, pulverized and sieved untreated bagasse samples and pretreated bagasse samples at 80°C to dry to constant weight, and conduct cellulose, semi For the determination o...

Embodiment 2

[0044] Embodiment 2: the pretreatment method of bagasse, concrete operations are as follows:

[0045] (1) After drying 40g of bagasse at 90°C for 54 hours, crushing and sieving to obtain raw materials with a particle size of 18-40 mesh;

[0046] (2) Add bagasse to NaOH and H 2 o 2 The mixed aqueous solution was added to the bagasse, and pretreated at 30°C with a 100rpm shaker for 12h, wherein the final concentration of NaOH in the mixed aqueous solution was 3.0%, H 2 o 2 The final concentration in the mixed aqueous solution is 1.0%, and the mass ratio of the mixed aqueous solution and bagasse is 20:1;

[0047] (3) Filter the pretreated bagasse, wash the filter residue with distilled water at 60°C until the filtrate is 6.5°C, and dry at 75°C to constant weight to obtain the pretreated bagasse for later use.

[0048] Detect by the detection method of embodiment 1: the relative content of cellulose, hemicellulose and lignin in the untreated bagasse raw material is respective...

Embodiment 3

[0049] Embodiment 3: the pretreatment method of bagasse, concrete operations are as follows:

[0050] (1) After drying 40g of bagasse at 85°C for 60 hours, crushing and sieving to obtain raw materials with a particle size of 18-40 mesh;

[0051] (2) Add bagasse to NaOH and H 2 o 2 The mixed aqueous solution was added to the bagasse, and pretreated at 35°C with a 200rpm shaker for 36h, wherein the final concentration of NaOH in the mixed aqueous solution was 1.0%, H 2 o 2 The final concentration in the mixed aqueous solution is 1.5%, and the mass ratio of the mixed aqueous solution and bagasse is 20:1;

[0052] (3) Filter the pretreated bagasse, wash the filter residue with distilled water at 65°C until the filtrate is neutral, and dry at 70°C to constant weight to obtain the pretreated bagasse for later use.

[0053] Detected according to the detection method of Example 1: the relative content of cellulose, hemicellulose and lignin in the untreated bagasse raw material...

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Abstract

The invention discloses a bagasse pretreatment method. The bagasse pretreatment method comprises the following steps of: drying, crushing and screening the bagasse, then pretreating the bagasse by using sodium hydroxide and hydrogen peroxide to obtain the pretreated bagasse. According to the pretreated bagasse material, the maximum removal rates of the hemicellulose and lignin respectively reach 78.18 percent and 94.56 percent, the maximum enzyme hydrolysis rate of the cellulose is 64.37 percent, and the enzymolysis efficiency is improved by 60.67 percent compared with that of untreated raw bagasse. Therefore, the pretreatment method breaks through wrapping of the hemicellulose and lignin to the cellulose, the hydrolysis rate of the cellulose is increased, the enzymolysis resistance property of the bagasse is improved, the obtained bagasse material can be used for converting high added value products, such as ethanol through microbial fermentation after being subjected to enzymolysis, and thus biomass resources, such as bagasse are effectively utilized.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and in particular relates to a bagasse pretreatment method. Background technique [0002] Lignocellulose is the most abundant and cheap renewable resource on earth, mainly derived from agricultural waste, industrial waste and municipal waste. With the depletion of fossil fuels and the deterioration of the environment, the use of lignocellulose to convert fuel ethanol and other energy products has attracted widespread attention. [0003] Bagasse is the part of sugarcane obtained after mechanical pressing and extraction, and is a by-product of cellulosic residue in the sugar cane sugar industry, accounting for about 24%-27% of the crushed amount. According to incomplete statistics, the total annual output of bagasse in my country has reached more than 7 million tons. Yunnan Province is one of the advantageous production areas of my country's sugar industry. Sugarcane sugar produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 伊日布斯魏治镭
Owner KUNMING UNIV OF SCI & TECH
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