72results about How to "Strong hydrolysis ability" patented technology

The invention belongs to the technical field of sulfur reclamation in the rubber ingredient industry, and in particular relates to a method for filling a sulfur production catalyst in the rubber ingredient industry, which is characterized in that the sulfur production catalyst is respectively filled into triple-stage sulfur production reactors, wherein the height of the catalyst in the triple-stage sulfur production reactors is all 800 millimeter; the upper part of a primary converter is filled with 1 cubic meter of PSR-41, and the lower part of the primary converter is filled with 2 cubic meters of PSR-31; the upper part of a secondary converter is filled with 1.1 cubic meters of the PSR-41, and the lower part of the secondary converter is filled with 2.1 cubic meters of the PSR-31; and the upper part of a tertiary converter is filled with 1.6 cubic meters of the PSR-41, and the lower part of the tertiary converter is filled with 1.6 cubic meters of the PSR-31. The method improves the sulfur conversion rate of a sulfur reclamation plant in the rubber industry.

The invention discloses a method for preparing silkworm chrysalis polypeptide by taking defatted silkworm chrysalis as raw material. It is characterized in that: it selects defatted silkworm chrysalis as raw material, takes composite enzyme of bromelain and neuter protease as proteolytic enzyme, carries out hydrolysis under special condition, and gets silkworm chrysalis polypeptide after drying. The invention is characterized by simple process, low production cost, long perservation time, ability of checking polypeptide content in silkworm chrysalis polypeptide through polypeptide content checking method, and the product is characterized by stable quality and ensured nutriment and effect. The product is widely used in food and health care food field, and can be matched with other animal, plant raw material with normal method to prepare capsule, soft capsule, granula, tablet and powder medicine.

The invention relates to a method for preparing queen bee embryo peptide by an enzyme hydrolysis method. The method comprises the steps of carrying out sample pretreatment, degreasing queen bee larva powder, preparing queen bee larva powder sample solution, treating the queen bee larva powder sample solution by ultrasonic waves, carrying out enzymatic hydrolysis reaction on the queen bee larva powder sample solution, heating enzymatic hydrolysate in a boiling water bath for 20min to realize enzyme deactivation, cooing to room temperature, adjusting pH value of the enzymatic hydrolysate to 7.0, centrifuging to take out supernate, and drying the supernate to obtain the hydrolyzed queen bee embryo powder. The prepared queen bee embryo polypeptide can be used for preparing health products for improving the function of human body, and more important, the bioactive polypeptides beneficial to human body can be screened out from the hydrolyzed queen bee embryo polypeptide.

The invention belongs to the technical field of sulfur reclamation in the rubber ingredient industry, and in particular relates to a method for filling a sulfur production catalyst in the rubber ingredient industry, which is characterized in that the sulfur production catalyst is respectively filled into triple-stage sulfur production reactors, wherein the height of the catalyst in the triple-stage sulfur production reactors is all 800 millimeter; the upper part of a primary converter is filled with 1 cubic meter of PSR-41, and the lower part of the primary converter is filled with 2 cubic meters of PSR-31; the upper part of a secondary converter is filled with 1.1 cubic meters of the PSR-41, and the lower part of the secondary converter is filled with 2.1 cubic meters of the PSR-31; and the upper part of a tertiary converter is filled with 1.6 cubic meters of the PSR-41, and the lower part of the tertiary converter is filled with 1.6 cubic meters of the PSR-31. The method improves the sulfur conversion rate of a sulfur reclamation plant in the rubber industry.

The invention discloses a method for preparing and extracting Bacillus subtilis debittering aminopeptidase by fermentation, which belongs to the technical field of enzyme preparation and food additive. The invention uses the fermentation cylinder production condition of Bacillus subtilis Zj016 to collect fermentation liquor and obtain the product of debittering aminopeptidase by carrying out clarifying, ultrafiltration concentration and salting out on the fermentation liquor. The aminopeptidase produced by the selected Bacillus subtilis Zj016 is exopeptidase which can dissociate amino acid from a polypeptide chain amino terminal. Researched by the laboratory, the aminopeptidase cooperates with alkali protease to hydrolyze soybean protein isolate; the prepared soybean peptide does not have bitterness, and the contents of oligopeptide like dipeptide and tripeptide are higher. Besides, researches on the enzyme lines produced by the Bacillus subtilis show that the Bacillus subtilis only produces circumscribed prolease and does not have the activity of endo protease; moreover, the circumscribed prolease has a stronger hydrolysis capacity to the terminal amino acid with strong hydrophobicity. The research further explains that the invention can reduce or eliminate the bitterness generated during hydrolysis of the soybean protein.

The invention provides a preservative agent for a fresh flower. The preservative agent is mainly prepared by adding glycerol and calcium chloride solution at certain concentrations to a 0.5% fibroin solution. The invention also provides a preservative method for the fresh flower and an application of the preservative agent in the course of red rose preservation. With the adoption of the preservative agent and the preservative method, air holes on the surfaces of petals can be closed appropriately, and a pervaporation flux of a membrane can be controlled, so that the effect of preserving the fresh flower is achieved.

The invention discloses an incision beta-1,3-glucanase coding gene from cellulosimicrobium cellulans, as well as enzyme, a preparation method and application of the incision beta-1,3-glucanase coding gene. The incision beta-1,3-glucanase coding gene is cloned to an escherichia coli expression vector through a technical method of gene engineering, so that an escherichia coli recombination strain capable of realizing heterologous expression of the enzyme can be obtained; incision beta-1,3-glucanase prepared through the heterologous expression of the strain can directly and efficiently degrade solid Curdlan. The invention further provides an antibiological inoculant, which is the incision beta-1,3-glucanase, for preventing and treating animal and plant diseases; the antibiological inoculant belongs to the field of biological prevention and treatment, and is expected to be novel biological pesticide. The incision beta-1,3-glucanase provided by the invention can be widely applied to the field of agriculture, food, feed additives, medicines, glucooligosaccharides preparation and the like.

The invention discloses a method for preparing low-bitterness whey protein antioxidative peptide powder. The method comprises the following steps: preparing whey protein aqueous solution; after high-temperature degeneration is carried out, adding neutral protease for carrying out one-time enzymolysis; adding ProteAX protease to carry out second-time enzymolysis; after enzymolysis is finished, carrying out high-temperature enzyme deactivation to obtain whey protein enzymatic hydrolysate; carrying out centrifugation and ultrafiltration treatment on the whey protein enzymatic hydrolysate, freezing, and drying to obtain the whey protein antioxidative peptide. In the method, the neutral protease and the ProteAX protease are adopted to carry out two-level enzymolysis on the whey protein to obtain the whey protein antioxidative peptide powder which has the advantages of high nitrogen recovery rate, small molecular weight, high antioxidant activity and low bitterness.

The invention discloses a neural stem cell cryoprotectant and a using method thereof. The cryoprotectant contains a DMEM / F12 basal culture medium, dimethyl sulfoxide, serum, albumin, a B27- additive,epidermal growth factors and alkaline fibroblast growth factors. The cryoprotectant is low in cost, and solves the problem that the anabiosis survival rate after cryopreservation of neural stem cellsis low; after the cells are cryopreservated for one year by adopting the neural stem cell cryoprotectant and the using method thereof disclosed by the invention, the cell viability still reaches up to95% higher, and the attribute and differentiative capacity of the neural stem cells are not influenced.

The invention relates to a method for a preparing po huo glycoside I refined product. The method includes the steps of using icariin (purity is larger than 98%) which is self-made through a laboratory as raw materials, carrying out hydrolysis of glucanase, obtaining a po huo glycoside I coarse product with content larger than 90%, using reverse phase efficient preparative chromatography as a purifying method, and obtaining the po huo glycoside I refined product with purity larger than 98%. The method is simple in process procedures, high in purity, low in cost, and suitable for mass production.

The invention provides a macadamia nut drink, which belongs to the technical field of drink preparation. The macadamia nut drink comprises the following materials: macadamia nut, xylitol, sorbitan, sucrose, citric acid, lactic acid, an emulsifier, a stabilizer, fungal protease, papain, aminopeptidase, and lactic acid bacteria; and the weight ratio of papain, aminopeptidase and lactic acid bacteriais: (0.1-0.2): (0.008-0.02): (0.2-0.3). The macadamia nut drink is obtained by the steps of pressing, pulping, enzymatic hydrolysis, debittering, blending, homogenization, fermentation, sterilizationand the like. The macadamia nut drink improves the nutrient content and stability of macadamia nut drink by using a reinforcing system composed of papain, aminopeptidase and lactic acid bacteria.

The invention discloses a method for producing an H-occupied BiVO4-OVs photocatalytic material and application of the H-occupied BiVO4-OVs photocatalytic material. The production method comprises thesteps of dissolving a certain molar weight of Bi(NO3)3.5 H2O in glycerin; dissolving a certain molar weight of NaVO3.2 H2O in deionized water; mixing solutions; transferring a mixed solution into a teflon-lined high-pressure kettle, and maintaining 180 DEG C for 8 h; conducting 10000-rpm centrifugal separation on a solvothermal synthesis product, washing the solvothermal synthesis product subjected to 10000-rpm centrifugal separation with deionized water and ethyl alcohol, and drying the washed solvothermal synthesis product subjected to 10000-rpm centrifugal separation at 60 DEG C for 4 h; calcining the solvothermal synthesis product, which is thoroughly washed, in a muffle furnace at 300 DEG C for 5 h; and conducting annealing on a calcined product in an Ar / H2 atmosphere at 350 DEG C for10 h to obtain the H-occupied BiVO4-OVs photocatalytic material. The H-occupied BiVO4-OVs photocatalytic material has the advantages that a photoresponse range is wide, the catalytic activity is high, the degradation rate is high, and the hydrolysis ability is high; and solar energy can be fully and effectively used.

The invention relates to the technical field of river snail aquaculture, in particular to a high-yield aquaculture method for river snails. The aquaculture method uses mountain spring as a river snail aquaculture water source, the water source is natural and pollution-free, and a good growth environment is provided for the river snails; a rice field is further modified to ensure flowing water in the rice field, and an environment suitable for growth of the river snails is created; reinforced management is performed, a filtering medicine package is arranged in a water inlet, and leeches can be prevented from entering an aquaculture pool from the source; the method uses traditional Chinese medicine powder to perform disinfection, disinfection of the rice field can be achieved, and the immunity of the river snails can be enhanced; and scientific formula of bait and a basal material can effectively promote growth of the river snails, and disease control and a high-yield objective can be achieved.

The present invention provides a vaccine composite adjuvant system and an application thereof in antigens. The vaccine composite adjuvant system is composed of an aluminum adjuvant, a double-strandedpolynucleotide-epsilon-polylysine complex and an aqueous solvent, wherein a clinical use concentration of the aluminum adjuvant is 0.1-10.0 mg / ml, and a clinical use concentration of the epsilon-polylysine complex based on double-stranded polynucleotide is 100-10,000 [mu]g / ml. The constructed compound adjuvant system has good anti-RNase hydrolysis ability, stability, safety and immune stimulatingactivity, is also combined with different forms of vaccines of hepatitis B vaccine, inactivated rabies vaccine, etc., and can also significantly improve immunogenicity of the vaccines.

The invention discloses a loach protein polypeptide and application thereof. The loach protein polypeptide is an antihypertensive peptide with the molecular weight being 1000-1300Da, and the antihypertensive peptide is prepared by the microwave-assisted enzymolysis technology. A preparation method of loach protein polypeptide includes: preprocessing, modifying protein, performing primary enzymolysis, performing secondary enzymolysis, deactivating enzymes, separating and purifying, performing high-pressure steam sterilizing, and concentrating and drying. By improving the enzymolysis process and technology of the loach protein polypeptide and using the microwave-assisted enzymolysis technology, the polypeptide with antihypertensive activity is prepared. The prepared loach protein polypeptide is high in yield and purity, can be used as the additive or active drug to be added to various industry and fields such as human food, drugs and healthcare products, and is significant to loach industrial chain prolonging, comprehensive increasing of loach industry comprehensive benefits, agriculture efficiency increasing, farmer income increasing, countryside prosperity and the promotion of one belt and one road construction.

The invention relates to a synthesis method of diglyceride. The method comprises the following steps: (1) mixing grease, lipase and a solvent, and carrying out hydrolysis reaction to obtain a hydrolysate; and (2) carrying out esterification reaction on the hydrolysate obtained in the step (1) and glycerol under the action of diglyceride lipase. The lipase in the step (1) is lipase MAS1 or MAS1-H108A. The synthesis method provided by the invention can be used for preparing the high-purity diglyceride, and is simple and convenient to operate and low in cost.

The invention discloses a method for preparing medical bone gelatin by a biological enzyme method and relates to a bone gelatin preparation process, particularly a process for preparing top grade medical bone gelatin by the biological enzyme method by taking animal bone meal such as pig bone meal, ox bone meal and the like as raw materials. The method is characterized by comprising the following steps: crushing; mixing slurry; dropping and soaking acid; washing; carrying out enzymolysis; re-cleaning; deactivating enzyme and extracting gelatin; flocculating and filtering; ultra-filtering and concentrating; and instantaneously sterilizing and the like. By taking small particle bone meal as the raw material, an excision enzyme method gelatin process of bone meal demineralization is accomplished under low acid concentration, and the process is verified successfully on a production line of producing 1 ton of gelatin one day. The production period is shortened to 2-3 days, the generated organic waste water is reduced by 80%, the production efficiency is improved to a great extent, and gelatin clean production is achieved. The produced and prepared gelatin product is high in jelly strength and good in viscosity and transparency and reaches the national standard of level A top grade medical gelatin by 100%.

A preparation method of a black soybean polypeptide honey beverage includes the steps of black soya bean selection and cleaning, pretreatment, pulping, enzymatic hydrolysis, centrifugation, homogenization and sterilization. Papain is employed for hydrolysis of black soya bean protein; and papain has extremely strong hydrolysis capability on protein, good heat resistance, and less demand on the hydrolysis conditions, and is conducive to the operation. The black soya bean polypeptide honey beverage prepared by the present invention has low molecular weight of peptides, high peptide content; addition of the honey reaches good taste of the product; and the black soya bean polypeptide honey beverage adapts to a large scope of population and has good product stability. The polypeptide in the product is easily soluble, easy to absorb, and can quickly regain their strength, and has good effects on resisting oxidation and fatigue, lowering blood pressure, improving the body's immunity and absorption functions.

This invention puts forward a method for producing compound biological active peptides by a casein enzyme method including preparing an enzymolysis substrate with casein, utilizing the compound trypsase and a neutral proteinase to carry out enzymolysis and controlling the hydrolyzed degree to 18-20%, then eliminating the enzymes, vacuum depressing and concentrating, the concentrated fluid is sprayed and dried to get the white-ivory-white powder compound active peptide product.

The invention discloses a saccharomyces cerevisiae strain and application thereof. The provided saccharomyces cerevisiae strain YLL19 is isolated from the natural fermented mash of Nanfeng tangerine oranges and preserved at the China Center for Type Culture Collection in Wuhan on March 18, 2018, and the preservation number is CCTCC. NO:M 2018139. The strain has not only good alcohol fermentation ability but also strong ability to hydrolyze hesperidin and degrade organic acid. The strain can be applied to biological fermentation and brewing of fruit wine; by using the strain for fermenting citrus pressed juice to brew citrus fruit wine, the bitterness can be effectively removed, the acidity can be effectively reduced, and the taste and quality of the citrus fruit wine are improved.

The invention relates to a method for immobilizing marine lipase Bohai Sea-9145 with magnetic Fe3O4@SiO2-NH2 nanoparticles. The method is characterized by comprising the following steps of: putting the magnetic Fe3O4@SiO2-NH2 nanoparticles in a reactor, then adding a marine lipase Bohai Sea-9145 solution with enzyme protein concentration of 0.0375-0.225mg / ml and pH value of 4.0-10.0 to the reactor to implement an immobilization reaction for 4-20 hours at 20-50 DEG C, then carrying out magnetic separation, collecting the supernatant for determining the content of the protein, and washing immobilized marine lipase Bohai Sea-9145 until no protein content in the washing solution is absorbed, thereby obtaining immobilized marine lipase Bohai Sea-9145. The method has the characteristics that the enzyme activity stability promotion effect of the immobilized marine lipase Bohai Sea-9145 is obvious, the enzyme activity recovery rate is as high as 87.7%, the obtained immobilized enzyme has good reutilization property and high mechanical strength and can still keep higher lipase activity after being stored for a long time, and the method is an ideal enzyme immobilization method; and therefore the method is more widely applied to industries, medicines, biochemical analysis and the like.

The invention relates to acid protease. The acid protease can hydrolyze protein under an acid condition, has a specific physiochemical characteristic and has high percent hydrolysis.

The invention relates to a health-care food with the taste of momordica grosvenori. The food is a liquid beverage health-care food prepared by mixing sodium bicarbonate powder, pearl powder, pappain, bromelin, melatonin and momordica grosvenori soaking liquid, and can also be a dry powder shape health-care food prepared by changing the momordica grosvenori soaking liquid into momordica grosvenori powder in the raw materials in the formula. The health-care food contains various ion mineral substances and trace elements, and the trace elements are in an ion status and are easy for a human body to absorb; the pH value of a product is in alkalescence, and the product is very effective for transmitting oxygen, regulating metabolism, removing acidic wastes and preventing diseases; and because the momordica grosvenori is added, regulating the mouth feel and having various effects, the invention is a purely natural health-care food suitable for the taste of common people beneficial to being popular among the common people.

The invention provides a method for preparing a bean drink by using immobilized enzyme, which comprises the following steps of: (1) immobilizing enzyme, namely immobilizing the enzyme by using calcium alginate gel to obtain immobilized protease; (2) preparing soybean culture solution, namely cleaning soybeans, boiling in water for 5 minutes, smashing to form thick liquid, adding water, standing, removing supernate, adding a stabilizing agent and glucose, and sterilizing to obtain the soybean culture solution; (3) fermenting, namely putting the immobilized protease in the step (1) into a flask, and fermenting in a shaker at constant temperature to obtain fermentation liquor; (4) filtering, and taking filter liquor; and (5) blending to obtain a finished product. In the method, a process is simple, the fermentation time is short, and large-scale industrial production is easy. The invention also provides a soybean drink which has good mouthfeel and abundant nutrition, and the high economic and social value.

The invention provides a high-absorptivity compound protein composition as well as a preparation method and application thereof, and belongs to the technical field of protein. The preparation method comprises the following steps: adding whey protein powder into a disodium hydrogen phosphate-citric acid buffer solution, inoculating lactobacillus plantarum, bacillus coagulans and bifidobacterium adolescentis for fermentation, adding compound protease into fermentation liquor for enzymolysis to obtain enzyme hydrolysate, further performing synchronous ultrasonic wave and ozone treatment, performing enzymolysis through cross-linked compound enzyme, and performing freeze-drying to obtain the whey protein powder. And adding a metal ion solution, heating, stirring and reacting to prepare the high-absorptivity composite protein composition. The high-absorptivity composite protein composition prepared by the invention is moderate in molecular weight, does not cause a first-pass effect of liver, has high absorption and utilization rate, is rich in protein function, and has obvious effects of resisting aging, resisting oxidation, promoting fat burning and promoting metabolism.

The invention discloses a titanium-based shift catalyst as well as a preparation method and application thereof. The titanium-based shift catalyst comprises 0.8-1.5% of CoO, 6-8% of MoO3, 5-8% of K2O,0-7% of CeO2 and the balance being TiO2 / SiO2. The novel titanium-based shift catalyst system provided by the invention has high activity at a low temperature (180-250 DEG C), and still has high activity and stability at a high temperature (250-450 DEG C). According to the preparation method, TiO2 / SiO2 is used as a carrier of the titanium-based shift catalyst, so that the vulcanization state of TiO2 / SiO2 can be kept, and the occurrence of reverse vulcanization can be avoided; a relatively high hydrolysis function is achieved on poison HCN, COS and CS2; the structural stability of the catalystis improved, and long-life operation is facilitated.

The invention discloses an acne-removing and whitening preparation as well as a preparation method and application thereof. Comprising the following raw materials in parts by weight: 4-6 parts of clove, 3-10 parts of leech, 12-18 parts of radix salviae miltiorrhizae, 10-15 parts of bletilla striata, 2-6 parts of white poria, 6-8 parts of carboxymethyl chitosan, 1-3 parts of a plumeria rubra extract, 1-5 parts of a bambusa vulgaris extract, 1-1.5 parts of octadecanedioic acid, 2-3 parts of sodium ascorbyl phosphate, 10-15 parts of papain, 3-4 parts of forsythin and 10-12 parts of grape seed oil. The acne-removing and whitening preparation is prepared through reasonable use and matching of various components and a special process, the synergistic effect is outstanding, melanin formation can be efficiently inhibited, the whitening effect is achieved, and meanwhile the acne-removing and whitening preparation has various outstanding effects of moistening skin, sterilizing, diminishing inflammation and the like.

The invention discloses a special enzyme used for a pet attractant, a zymolyte used for the pet attractant, the pet attractant and production methods of the special enzyme used for the pet attractant,the zymolyte used for the pet attractant and the pet attractant, and relates to the technical field of enzyme preparations. The special enzyme comprises: 1-20 parts of alkaline protease, 1-4 parts offlavourzyme, 1-10 parts of papain, 1-6 parts of lipase, 1-20 parts of neutral protease, 1-20 parts of acid protease and 1-100 parts of a carrier. The inventor coordinates the multiple enzymes and reasonably deploys the usage amounts of the multiple enzymes to make a synergistic effect among the various enzymes more significant and to make the hydrolysis ability stronger, an obtained enzymolysis product does not have bitter taste or astringent taste, and the palatability is high. According to the zymolyte and the production method thereof, by adopting the special enzyme for conducting enzymolysis treatment on meat, the flavor of the enzymolysis product can be significantly improved, and subsequent production of the pet attractant with better flavor and taste is facilitated. According to the pet attract and the production method thereof, the flavor and the taste of the pet attractant produced by adopting the zymolyte as a raw material are significantly improved.

A synthesis method for simultaneously preparing a positive electrode material and a negative electrode material of a high-performance supercapacitor by taking a Co-MOF array as a precursor comprises the following steps: firstly, respectively dissolving Ni (NO3) 2.6 H2O and 2-MI in water according to a molar ratio of 1: (3-9), and vertically immersing 1 * 1cm foamed nickel (NF) or foamed iron nickel (FNF) to obtain Co-MOF-coated NF and Co-MOF-coated FNF; then soaking the Co-MOF coated NF in an ethanol solution containing 76 mg of Ni (NO3) 2 to prepare CN-LDH, and then soaking the CN-LDH in an aqueous solution containing 1-12 mmol L <-1 > of NaVO3 for standing to obtain CNV; the Co-MOF coated FNF is soaked in a Fe (NO3) 3.9 H2O aqueous solution with the concentration of 1-6 mg mL <-1 > to obtain CF-LDH, then the array is soaked in a NaVO3 aqueous solution with the concentration of 1-12 mmol L <-1 > to stand, and the CFV is obtained. CNV or CFV is immersed in a TAA solution containing 2.5 mg mL <-1 > to 10 mg mL <-1 >, a reaction is carried out for 12 h at the temperature of 160 DEG C, and a target product CNVS-x or CFVS (x = TAA solution concentration / 2.5) is obtained. According to the preparation strategy for constructing two trimetal sulfide composite materials based on the same Co-MOF precursor, the electron transfer rate is increased, and the energy density of the supercapacitor is enhanced.

The present invention provides a vaccine composite adjuvant system and applications thereof in antigens. The system is composed of an aluminum adjuvant, a double-stranded polynucleotide-epsilon-polylysine-sulfuric acid glycan compound and an aqueous solvent, wherein the clinical use concentration of the aluminum adjuvant is 0.1-10.0 mg / ml, and the clinical use concentration of the double-strandedpolynucleotide-epsilon-polylysine-sulfuric acid glycan compound is 100-10000 [mu]g / ml by double-stranded polynucleotide. The formed compound adjuvant system has good anti-RNase hydrolysis ability, stability, safety and immune stimulating activity, and can significantly improve immunogenicity of vaccine when combined with different forms of vaccines such as hepatitis B vaccine and inactivated rabies vaccine.