351 results about "Icariin" patented technology

The invention relates to a method for preparing icaritin and is characterized in that: the icaritin is prepared by icariin which is subject to the enzymolysis reaction by beta-glycosidase. The method adopts the beta-glycosidase to perform the enzymolysis reaction for the icariin, the beta-glycosidase has complete deglycolysis and high yield, a self-designed method for extracting the icariin from Herba Epimedii is adopted, the whole technical process has simple and convenient operation, the treatment is convenient after the reaction, and the purity of the product fully meets the pharmaceutical standard.

The invention relates to a herba epimedii aglycone phospholipid compound or a cyclo-herba epimedii aglycone phospholipid compound, which is characterized by being composed of herba epimedii aglycone or cyclo-herba epimedii aglycone and phospholipid, wherein the mass ratio of the herba epimedii aglycone or the cyclo-herba epimedii aglycone and the phospholipid is 1:0.5 to 5. The phospholipid in theherba epimedii aglycone phospholipid compound or the cyclo-herba epimedii aglycone phospholipid compound has the positive function on treating the osteoporosis, and the medicine and the phospholipidin the phospholipid compound of the herba epimedii aglycone or cyclo-herba epimedii aglycone medicine has the synergic function on treating the osteoporosis; after the phospholipid and the herba epimedii aglycone or the cyclo-herba epimedii aglycone are prepared into the compound, the dissolvability thereof is improved greatly, and the disadvantages of poor water solubility and fat solubility andlow bioavailability are solved; the herba epimedii aglycone phospholipid compound or the cyclo-herba epimedii aglycone phospholipid compound has simple preparation process and moderate reaction condition, and is suitable to industrialized big production. The invention discloses a preparation method thereof.

The invention provides icariin application in inducing stem cell in vitro orientation differentiates to several single type cells. The stem cell said includes embryonic stem cell, nerve stem cell and marrow mesenchyme stem cell. The single type cell includes nerve cell, bone cell, islet cells and endothelial cell. This invention also relates to application of the single type cell in preparing medicine of stem cell transplant curing nerve degenerative diseases, and its application in preparing cell differentiation agent that used to repair recovery injured nerve tissue, and its application in high efficiency drug effect screening model rebuilding and initial screening and estimating by using the model. The new applications of the icariin is extended in this invention, the fact of icariin contains pharmacy activity clarified, so it provides material basis for Chinese traditional medicine prevention and cure effect. The clarifying of drug effect mechanism provides reference for new medicine Chinese metical modern development with self-owned intellectual property right.

A pharmaceutical composition containing icariin as an active component for preventing and treating sexual dysfunction and vasculargenic disease is disclosed as is the use of icariin as an active component for the manufacture of such pharmaceutical compositions. Also disclosed is a method for the prevention or treatment of sexual dysfunction and vasculargenic disease which comprising administrating an effective amount of icariin or a pharmaceutical composition containing icariin to a patient who stands in need of such treatment.

The invention provides a total synthesis method for preparing the icariin compound with a structural formular as (I); R<1> symbolizes H or Rham and R<2> symbolizes H or Glc. By adopting the Claisen rearrangement reaction triggered by the microwave, the method leads the isopenteny in with positioning on the flavone ring with high selectivity so as to enhance the overall yield and lead to the easily obtained raw materials and higher yield of the icariin compound. The invention is suitable for industrialized production and solves the problem that the compound can be only prepared through extraction and separation in the Chinese herbal medicine extractum with extremely low efficiency.

The invention relates to a method for preparing icaritin through converting total flavones of epimedium by an enzyme method. The method comprises the step of converting multicomponent flavone glycosides in the total flavones of epimedium by a dual-enzyme system consisting of heat-resistant alpha-L-rhamnosidase and heat-resistant beta-glucosidase, so as to produce the icaritin. According to the method, multicomponent flavone compounds (such as icariin, Epimedin A, Epimedin B and Epimedin C) in the total flavones of epimedium can be almost completely converted into the target product; the optimal acting temperature is high, so that a substrate and intermediate products have good solubility, and a cosolvent is not required; and the enzymolysis time is short, and the yield is high. The icaritin prepared by the method has a remarkable higher propagation inhibiting effect on liver cancers, lung cancers, colon cancers and mammary cancers compared with each monomer of the total flavones of epimedium before conversion.

The invention relates to a method for preparing icariin, which comprises the following process steps: ethanol extraction, concentration, low-alcohol macroporous resin enrichment, polyamide separation and purification, and recrystalization. When the method is used for producing the icariin, the process is simple, the product yield is high, and the product purity is high. The process is also suitable for producing icariin of different content levels; and the product prepared by the process meets food and medical requirements.

The invention discloses chitosan-hydroxylapatite in-situ loaded icariin composite microspheres and a preparation method thereof. The preparation method is characterized in that a micro-capsule forming device is adopted, chitosan is used as an organic substrate, and soluble calcium salt and soluble phosphate are used as precursors of inorganic phase nanometer hydroxylapatite; the in-situ crystal induction effects of hydroxyl radicals and amino radicals in the chitosan on the hydroxylapatite are used for adjusting and controlling the in-site heterogeneous nucleation crystallization of an inorganic mineral on the organic substrate; and meanwhile, a traditional Chinese medicine, namely icariin which has the effect of promoting proliferation and differentiation on osteoblast is compounded, and the chitosan-hydroxylapatite in-situ loaded icariin composite microspheres are prepared through in-situ simulation. The chitosan-hydroxylapatite in-situ loaded icariin composite microspheres are bone filling materials, have good biocompatibility and biodegradability and are expected to serve as repair materials for bone tissue defects to be widely applied in clinic; the preparation processes are simple and easy; and the method is mild.

The invention discloses an application and product of icariin in the drug to treat senile dementia, which is characterized by the following: extracting icariin from berbere epimedium plant as flavonol compound; adopting icariin as raw material to prepare the monoclinic agent with simple technique and low cost; fitting for treating senile dementia with obvious effect and little side effect.

The invention discloses autofluorescence bone repairing magnetic sustained-release microspheres and a preparation method thereof. The method comprises the steps of synthesizing nano Fe3O4 by a solvothermal method; uniformly dispersing nano Fe3O4 into a sodium alginate solution while combining with a traditional Chinese medicine-icariin with proliferation and differentiation effects on osteoblast; enabling the mixture to pass through a microcapsule forming device, and dropwise adding the mixture into a gel bath to form primary microspheres; soaking the primary microspheres in a chitosan solution to form a polyelectrolyte semipermeable membrane by use of the charge complementation feature of chitosan and sodium alginate; crosslinking the composite microspheres with genipin to obtain the autofluorescence bone repairing magnetic sustained-release microspheres. According to the autofluorescence bone repairing magnetic sustained-release microspheres and preparation method thereof disclosed by the invention, the requirements of an implanted bone repairing material on cytotoxicity are taken into full consideration, the chitosan and sodium alginate with excellent biocompatibility and degradability are selected as raw materials, and the microspheres with different particle sizes can be prepared by adjusting the voltage according to the needs of the bone lesion.

The invention provides a method of converting icariin into anhydroicaritin by using a biological enzyme reaction so as to improve biological activity of icariin. According to the invention, under the action of naringinase, a reaction is carried out in an ethanol aqueous solution with a concentration of 30% to 70%, wherein, reaction temperature is 40 to 70 DEG C and reaction time is 1 to 30 h; glycosyl groups on hydroxy groups of icariin are cut off, and icariin is converted into anhydroicaritin with higher pharmacological activity; furthermore, machinability of icariin is improved, and the practical application scope of icariin can be enlarged.

The invention relates to alpha-L-rhamnosidase and application thereof. The amino acid sequence is shown as SEQ ID NO.2. The alpha-L-rhamnosidase BtRha capable of specifically degrading alpha-1,2 glycosidic bonds is cloned and recombinantly expressed for the first time, and a blank of the alpha-L-rhamnosidase for hydrolyzing the alpha-1,2 glycosidic bonds between rhamnose is filled up. The alpha-L-rhamnosidase can efficiently and specifically degrade epmedin C and convert the epmedin C into icariin, and the molar conversion rate of 1 g / L of the epmedin C hydrolyzed by 1.5 U / mL of the alpha-L-rhamnosidase is as high as 90.5% or above under the conditions that the pH is 5.5 and the temperature is 55 DEG C.

The invention relates to an icariin microemulsion which takes icariin as active ingredient; the icariin is dissolved in oil and then added with emulsifying agent, assistant emulsifier and water to prepare microemulsion with the micro emulsion grain diameter of 10-100nm, wherein, the oil can be one or more in oleic acid, ethyl oleate, isopropyl myristate or castor oil; the icariin microemulsion has the components with the mass percent: 0.66-6.02% of saturated solution of oil of icariin, 10-50% of emulsifying agent, 10-38% of assistant emulsifier and the balance water, Hank's balanced salt solution (HBSS), PBS buffer solution or normal saline. The icariin microemulsion can be kept clear, transparent, stable and uniform when being placed at room temperature for a year, thus good stability is verified evidently. The experiments of cells and animals prove that the icariin microemulsion can strengthen the icariin to penetrate cell layers, promote the medicine to be ingested and absorbed and enhance the healing effect of the medicine. The invention also discloses a preparation method of the icariin microemulsion.

The invention relates to a preparation method of hydrated icariin which belongs to the synthesis field of natural compounds. The preparation method of the hydrated icariin is characterized in that the hydrated icariin is obtained from icariin after two steps of the hydrolysis reaction of hydrochloride and cellulose. The desugarization hydrolysis is complete. The chemical structure of barrenwort aglycone can be changed, which changes the barrenwort aglycone into the hydrated icariin. The yield is high. The treatment after the reaction is convenient. And the purity of products fully complies with the medical standards.

The invention provides a preparation method of baohuoside I, which has a high efficiency and a high conversion rate. The method is characterized in that baohuoside I is obtained by treating a certain concentration of ethanol as a reaction medium, and carrying out enzyme hydrolysis on icariin. The method which adopts a low concentration of an ethanol solution as the reaction medium has the advantages of increase of the specific surface area of a contact reaction of icariin with an enzyme, shortening of the reaction time, improvement of the conversion efficiency and the conversion rate, simple and feasible technology, good purposiveness, no pollution, and realization of industrial production, and overcomes disadvantages of complex operation, much time consumption, low conversion rate, bad purposiveness, difficult separation and purification, and the like existing in the prior art.

The invention discloses medicinal application and an extracting method of shorthorned epimedium P.E, belonging to the field of medicines. The shorthorned epimedium P.E containing icariin, icariin A, icarisid II and epimedin B is applied to preparing medicines for treating chronic bacterial prostatitis, chronic nonbacterial prostatitis and hyperplasia of the prostate. The preparation method comprises the following steps: adding water or ethanol to epimedium for extraction; concentrating the extracting solution until the relative density is 1.01-1.10 at the temperature of 70 DEG C; and refining the filter liquor by a polyamide adsorption method, thus obtaining the shorthorned epimedium P.E. The invention has the following beneficial effects: by comparing data obtained in pharmacological tests with the data in related documents and patents, the medicines for treating chronic prostatitis and chronic hyperplasia of the prostate, which are prepared from the ehorthorned epimedium P.E, have definite curative effect, have no side effects and also have treatment effect superior to that of the prior art.

A composition including an aliquot of the herb Herba Epimedii; and an aliquot of at least three supplemental herbs selected from the group consisting of Fructus Rosae Laevigatae; Fructus Rubi; Fructus Psoralea; Radix Morindae Officinalis; Fructus Schisandrac Chinensis; Fructus Ligustri Lucidi; Semen Cuscutae; and Radix Astragali. A composition including icariin; ursolic acid; ellagic acid; psoralen; deoxyschizandrin; oleanolic acid; quercetin; aslvagaloside; and an extract of the herb Radix Morindae Officinalis. Methods including administering a composition directed at treatment of various kidney disorders or the promotion of kidney health and to the overall health of the kidney, including the use of a composition in the treatment of prostate cancer, prophylatic prostate health, reduction of polyuria, incontinence, proteinuria, as well as for sexual satisfaction.

The invention provides a herba epimedii extract, in particular to a high-content herba epimedii total flavonoids extract, and the preparation method thereof mainly comprises the following steps: (1) alkali-ethanol-water is taken as a menstruum for refluxing extraction; (2) decompression and concentration are carried out; (3) pH value is adjusted; (4) macroporous resin column elution is carried out; and (5) an eluant is dried and the content of the obtained total flavonoids is over 65 percent and the content of icariin is over 20 percent. The extract can be prepared into various oral preparations according to the requirements in pharmacy, which can be hard capsules, soft capsules, conventional tablets, orally disintegrating tablets, buccal tablets, dispersible tablets, sustained-release preparations, controlled release preparations, granules, water-paste pills, dripping pills, honeyed pills, and the like. The extract and effective medicines containing the extract can be applied to preparing brain tissue protective agents, especially to preparing medicines that prevent and treat cerebral infarction, cerebral infarction sequela and other ischemic cerebrovascular diseases.

The invention discloses a health food used to improve sexual functions of both male and female. The health food contains the following weight percentage of components: 30 to 50 portion of L-arginine, 6 to 10 portion of icariin, 3 to 6 portion of Fructus Tribuli and 5 to 15 portion of taurine. After lots of animal and human body function tests, the health food of the invention used to improve sexual functions of both male and female causes no side effects after long term of consumption, has good tolerance without changing hormone balance of human body.

A composition including an aliquot of the herb Herba Epimedii; and an aliquot of at least three supplemental herbs selected from the group consisting of Fructus Rosae Laevigatae; Fructus Rubi; Fructus Psoralea; Radix Morindae Officinalis; Fructus Schisandrac Chinensis; Fructus Ligustri Lucidi; Semen Cuscutae; and Radix Astragali. A composition including icariin; ursolic acid; ellagic acid; psoralen; deoxyschizandrin; oleanolic acid; quercetin; aslvagaloside; and an extract of the herb Radix Morindae Officinalis. Methods including administering a composition directed at treatment of various kidney disorders or the promotion of kidney health and to the overall health of the kidney, including the use of a composition in the treatment of prostate cancer, prophylatic prostate health, reduction of polyuria, incontinence, proteinuria, as well as for sexual satisfaction.

The invention relates to a quick detection method for Wangbi preparation dry intermediate multiple indexes. The method comprises the steps of: (1) determining the content of Wangbi intermediate standard preparation drying moisture, albiflorin, paeoniflorin, 5-O-methylvisammioside, icariin and Epimedin C in Wangbi preparation dry intermediate standard substances; (2) acquiring infrared diffuse reflection spectrums of the standard substances; (3) conducting pretreatment on the spectrums; (4) establishing a content prediction model by partial least square regression; (5) collecting the infrared diffuse reflection spectrum of a to-be-detected sample and performing pretreatment; and (6) substituting the pretreatment spectral data of the to-be-detected sample into the model obtained in step (4), thus acquiring predicted values of corresponding component contents. The method provided by the invention introduces the near infrared spectroscopic analysis technology into quality control of Wangbi preparation intermediates, realizes rapid determination of various quality control index contents, shortens the detection time, saves the production cost, improves the production efficiency and economic benefits, and ensures the safety and effectiveness of Wangbi tablets.

The invention discloses a method for preparing herba epimedii low-glycoside components from herba epimedii. By means of an in-vitro enzymolysis technology, enzymolysis and desugaring are carried out on herba epimedii flavonoid polysaccharide obtained from alcohol extraction herba epimedii decoction pieces, and the herba epimedii low-glycoside components are obtained through conversion. The methodis high in enzymolysis conversion rate, easy and convenient to operate, environmentally friendly, economical and feasible. Polysaccharide substances, such as epmedin A, epmedin B, epmedin C and icariin, in herba epimedii total flavonoids can be completely enzymolyzed and converted into low-glycoside compounds, baohuoside II, sagittatoside A, sagittatoside B, rhamnose-based icarisid II, anhydroicaritin-3-O-rhamnose-dideoxyfuranose and baohuoside I. A stable composition structure ratio is achieved, so that the content of the herba epimedii low-glycoside components is increased.

The invention provides a novel application of icariin. Expression of a pluripotent gene Oct-4mRNA can be improved after the icariin acts on an umbilical cord mesenchymal stem cell after a week, pluripotency of the umbilical cord mesenchymal stem cell is improved, the capacity of the umbilical cord mesenchymal stem cell differentiating a nerve cell, a myocardial cell and an islet cell can be improved, the methylation level of the pluripotent adjusting gene Oct-4 is reduced, and the acetylation level of histone H3K9 is promoted.

The present invention provides a new use icariin in inducing external oriented differentiation of embryo stem cells into single type of cells. Icariin may be used as the mitogen in stem cell transplanting cardiac muscle regeneration or cardiac muscle cell reconstruction, and in constructing high-efficiency pharmacodynamic screening model for initial screening and estimation of medicine effect. The present invention demonstrates the medicine effect of icariin in cell level and approaches its plentiful biological information. The present invention determines icariin as pharmacological active componnet and this provides Chinese medicine prevention and treatment with substantil foundation. At the same time, the description of the pharmacodynamic mechanism provides reference for modernized development of Chinese medicine.

The invention relates to an antifatigue healthcare composition comprising the following active components in parts by weight: 20-50 parts of icariin, 5-20 parts of astragaloside, 20-40 parts of sealwort extractive and 10-30 parts of American ginseng extractive. The invention further discloses a preparation method of the composition. The antifatigue healthcare composition has the functions of improving exorbitance of brainwork and physical work, tonifying Qi, nourishing yin, tonifying spleen, moistening lung, remarkably refreshing brain, improving memory, boosting immunity, slowing down aging and the like.

The invention relates to the method for detecting the quality of senile cough and cough tablets. The method comprises the following steps: qualitatively distinguishing the active ingredients including radix astragaliseuhedysari, bighead atractylodes rhizome, radix saposhnikoviae, licoriceroot,herbaepimedii andfructuspsoralea in asenile cough and cough tabletpreparation by using a thin layer chromatography, and determining the contents of icariin, psoralen and isopsoralenin the preparation by a UPLC method. The detection method provided by the invention has the advantages of simplifying the sampling steps, having simple operation and high specificity, shortening the content determination time, simultaneously detecting multi-index components, having stabledetection baseline, good resolution, high detection accuracy and good stability, improving efficiency, and being relatively economic and environmentally friendly.

The invention provides white spirit with an anti-fatigue function, wherein 0.15g-0.16g of ginseng extract, 0.02g-0.03g of astragalus root extract, 0.10g-0.15g of horny goat weed extract and 2.0g-2.5g of wolfberry extract are added to 33-68%Vol white spirit per liter so that the content of total saponins of panax ginseng is greater than or equal to 60mg / L, the content of icariin is greater than or equal to 20mg / L, the content of astragaloside is greater than or equal to 5mg / L and the content of glycine betaine is greater than or equal to 490mg / L in the white spirit by Re. The white spirit with the anti-fatigue function has the advantages that ginseng, astragalus root, horny goat weed and wolfberry are taken as raw materials and the active ingredients total saponins of panax ginseng, astragaloside, icariin and glycine betaine of the raw materials are extracted and added to the white spirit, respectively, and therefore, the original flavor, color and luster as well as taste of the white spirit are remained, the white spirit is clear in ingredients and stable in content, and meanwhile, the white spirit has accurate anti-fatigue function; the white spirit with the anti-fatigue function is yellowish in color, aromatic and elegant, soft, lasting sweet and smooth, harmonious in various aromas, and smooth and clear in taste; judged by professional wine taster, the white spirit with the anti-fatigue function is close to common kaoliang spirit or pure grain wine in style.

The invention provides a method for synchronously extracting and separating icariin and icarisid II from herba epimedii. The method obtains high-purity extract of icariin and icarisid II through macroporous adsorption resin gradient elution respectively. The invention also provides a method for extracting and separating icariin or icarisid II. The method provided by the invention not only realizes synchronous extraction and separation of icariin and icarisid II and simplifies the technological process, but also obtains a medicinal intermediate with relatively high purity, obviously increases the yield of monomer components, improves the utilization rate of medicinal materials and reduces the production cost; and meanwhile, without using organic solvents with relatively high toxicity, the method guarantees the safety of operators, realizes the green production and has great industrial application prospects.

Disclosed is an anti-tumor drug with nor-icariin as the effective ingredient. Pharmacological test effect proves that the nor-icariin has the activity to inhibit the production of cellular lipid, can reduce the production of cellular fatty acid by inhibiting the activity of FAS, has a function of inhibiting the production and causing withering of microvascular endothelial cells, shows a broad-spectrum anti-tumor function in a cytotoxicity test of thirty-three tumor cell strains of a human body, and shows a function in strongly inhibiting the production of tumor, such as prostate cancer cells LnCAP, lung cancer cells NCI-H23 and colon cancer cells HCT-116 of a human body in a tumor transplantation nude mice model test. A pharmacokinetics test of the nor-icariin in the body of a little mouse proves that the nor-icariin can be kept with a certain concentration, good for better anti-humor effect. The acute toxicity test of the nor-icariin in the body of the mouse shows nontoxicity; therefore the nor-icariin is proved to be a newly-developed broad spectrum anti-humor drug with broad development prospect.