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Autofluorescence bone repairing magnetic sustained-release microspheres

A slow-release microsphere and autofluorescence technology, which is applied in the field of bone filling material manufacturing, can solve the problems of high cytotoxicity of cross-linking agents and affect normal tissue growth, etc., and achieves superior biocompatibility, simple preparation method, and bone defect diseases. The effect of promotion

Inactive Publication Date: 2014-01-08
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the traditional cross-linking methods use chemically synthesized cross-linking agents. The cross-linking agents themselves have relatively high cytotoxicity, causing the obtained biomaterials to affect the growth of normal tissues after they are implanted into the receptors.

Method used

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  • Autofluorescence bone repairing magnetic sustained-release microspheres
  • Autofluorescence bone repairing magnetic sustained-release microspheres
  • Autofluorescence bone repairing magnetic sustained-release microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Add 1.35g FeCl 3 ·6H 2O, 40ml ethylene glycol, 3.6g sodium acetate, magnetically stirred evenly, placed in a reaction kettle, after 12 hours of reaction, the obtained product was washed several times with absolute ethanol, several times with deionized water, centrifuged, 40°C vacuum drying;

[0044] (2) Sodium alginate was dissolved in deionized water with a mass percentage of 1.2%, and magnetically stirred for 1 hour;

[0045] (3) Add the prepared nano-magnetic Fe to the above solution 3 o 4 Powder, the mass percentage is 0.008%, electric stirring for 1h;

[0046] (4) Add an ethanol solution of icariin, with a mass percentage of 0.02%, and magnetically stir for 1 hour;

[0047] (5) Using a microcapsule forming device with a voltage of 1.3kv, use a syringe to drop the mixture in step (4) into the gel bath to form primary microspheres;

[0048] (6) Submerge the primary microspheres in a chitosan acetic acid solution with a mass percentage of 0.6% for 8 hours, a...

Embodiment 2

[0051] (1) Add 1.35g FeCl 3 ·6H 2 O, 40ml ethylene glycol, 3.6g sodium acetate, magnetically stirred evenly, placed in a reaction kettle, after 12 hours of reaction, the obtained product was washed several times with absolute ethanol, several times with deionized water, centrifuged, 40°C vacuum drying;

[0052] (2) Sodium alginate was dissolved in deionized water with a mass percentage of 1.4%, and magnetically stirred for 1 hour;

[0053] (3) Add the prepared nano-magnetic Fe to the above solution 3 o 4 Powder, the mass percentage is 0.008%, electric stirring for 1h;

[0054] (4) Add an ethanol solution of icariin, with a mass percentage of 0.03%, and magnetically stir for 1 hour;

[0055] (5) Using a microcapsule forming device with a voltage of 1.3kv, use a syringe to drop the mixture in step (4) into the gel bath to form primary microspheres;

[0056] (6) Submerge the primary microspheres in a chitosan acetic acid solution with a mass percentage of 0.8% for 10 hours,...

Embodiment 3

[0059] (1) Add 1.35g FeCl 3 ·6H 2 O, 40ml ethylene glycol, 3.6g sodium acetate, magnetically stirred evenly, placed in a reaction kettle, after 12 hours of reaction, the obtained product was washed several times with absolute ethanol, several times with deionized water, centrifuged, 40°C vacuum drying;

[0060] (2) Sodium alginate was dissolved in deionized water with a mass percentage of 1.5%, and magnetically stirred for 1 hour;

[0061] (3) Add the prepared nano-magnetic Fe to the above solution 3 o 4 Powder, the mass percentage is 0.01%, electric stirring for 1h;

[0062] (4) Add an ethanol solution of icariin, with a mass percentage of 0.03%, and magnetically stir for 1 hour;

[0063] (5) Using a microcapsule forming device with a voltage of 1.3kv, use a syringe to drop the mixture in step (4) into the gel bath to form primary microspheres;

[0064] (6) Submerge the primary microspheres in a chitosan acetic acid solution with a mass percentage of 0.5% for 12 hours, ...

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Abstract

The invention discloses autofluorescence bone repairing magnetic sustained-release microspheres and a preparation method thereof. The method comprises the steps of synthesizing nano Fe3O4 by a solvothermal method; uniformly dispersing nano Fe3O4 into a sodium alginate solution while combining with a traditional Chinese medicine-icariin with proliferation and differentiation effects on osteoblast; enabling the mixture to pass through a microcapsule forming device, and dropwise adding the mixture into a gel bath to form primary microspheres; soaking the primary microspheres in a chitosan solution to form a polyelectrolyte semipermeable membrane by use of the charge complementation feature of chitosan and sodium alginate; crosslinking the composite microspheres with genipin to obtain the autofluorescence bone repairing magnetic sustained-release microspheres. According to the autofluorescence bone repairing magnetic sustained-release microspheres and preparation method thereof disclosed by the invention, the requirements of an implanted bone repairing material on cytotoxicity are taken into full consideration, the chitosan and sodium alginate with excellent biocompatibility and degradability are selected as raw materials, and the microspheres with different particle sizes can be prepared by adjusting the voltage according to the needs of the bone lesion.

Description

technical field [0001] The invention belongs to the field of bone filling material manufacture, and in particular relates to autofluorescent bone repair magnetic slow-release microspheres and a preparation method thereof. Background technique [0002] Trauma, bone tissue inflammation, bone defects caused by congenital defects, and bone tissue diseases such as bone cancer are very common in orthopedic clinics. Relying solely on the self-repair ability of bone is relatively limited. Bone transplantation is often used clinically to treat bone tissue diseases. Therefore, the research and development of bone repair materials is of great significance. In recent years, more and more attention has been paid to the research of microspheres and microcapsules in the field of biomedicine. Microsphere is a kind of spherical or quasi-spherical particle made of suitable polymer material as the carrier. The particle size is generally 1-500 μm, the small one can be several nanometers, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/12A61L27/20A61L27/54A61L27/50B01J13/02
Inventor 陈景帝张惠张玉珏潘盼盼张其清
Owner FUZHOU UNIV
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