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Chitosan-hydroxylapatite in-situ loaded icariin composite microspheres

A technology of hydroxyapatite and icariin, which is applied in the field of biomedical materials, can solve the problems of unprepared microspheres and the like, and achieve the effects of promoting wound healing, convenient operation and promoting bone formation.

Inactive Publication Date: 2014-01-08
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, many studies have focused on compounding hydroxyapatite and chitosan to form bulk scaffold materials, and there are also literature reports on mechanically mixing the two to form microspheres, but there is no report on the in situ compounding of the two. Method for preparing microspheres

Method used

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  • Chitosan-hydroxylapatite in-situ loaded icariin composite microspheres
  • Chitosan-hydroxylapatite in-situ loaded icariin composite microspheres
  • Chitosan-hydroxylapatite in-situ loaded icariin composite microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0034]1) Prepare 1.5mol / L soluble calcium salt solution and 1.8mol / L soluble phosphate solution; the contents of chitosan and icariin are respectively 2% and 0.01% by mass percentage;

[0035] 2) dissolving the chitosan in the ratio in 1% acetic acid solution, and dissolving the icariin in the ratio in absolute ethanol;

[0036] 3) According to n(Ca 2+ ):n(PO 4 3- )=1.67:1 ratio, a certain amount of soluble calcium salt solution and soluble phosphate solution were added to the above-mentioned mixed solution respectively, continuously stirred for 2 h, and the mass ratio of theoretically forming HAP was 1%;

[0037] 4) Slowly add the icariin alcohol solution to the above solution, stir to make it fully mixed;

[0038] 5) Put the above mixed solution through a microcapsule forming device, drop it into a 3% NaOH aqueous solution to form it, put it together with the gel bath in a 37°C water bath for 8 hours, carry out in-situ crystallization, and then repeatedly soak it with dei...

Embodiment 2

[0040] 1) Prepare 2mol / L soluble calcium salt solution and 1.2mol / L soluble phosphate solution; the contents of chitosan and icariin are respectively 1% and 0.03% by mass percentage;

[0041] 2) dissolving the chitosan in the ratio in 1% acetic acid solution, and dissolving the icariin in the ratio in absolute ethanol;

[0042] 3) According to n(Ca 2+ ):n(PO 4 3- )=1.67:1 A certain amount of soluble calcium salt solution and soluble phosphate solution were added to the above mixture respectively, and stirred continuously for 3 h, the theoretical mass ratio of forming HAP was 0.5%.

[0043] 4) Slowly add the icariin alcohol solution to the above solution, stir to make it fully mixed;

[0044] 5) Pass the above mixed solution through a microcapsule forming device, drop it into a 3% NaOH aqueous solution to form it, and place it together with the gel bath in a 37°C water bath for 10 hours for in-situ crystallization, and then repeatedly soak it with deionized water until Neut...

Embodiment 3

[0046] 1) Prepare 3mol / L soluble calcium salt solution and 0.6mol / L soluble phosphate solution; the contents of chitosan and icariin are respectively 2% and 0.05% by mass percentage;

[0047] 2) dissolving the chitosan in the ratio in 1% acetic acid solution, and dissolving the icariin in the ratio in absolute ethanol;

[0048] 3) According to n(Ca 2+ ):n(PO 4 3- )=1.67:1 ratio, a certain amount of soluble calcium salt solution and soluble phosphate solution were added to the above-mentioned mixed solution respectively, continuously stirred for 4 h, and the mass ratio of theoretically forming HAP was 0.8%;

[0049] 4) Slowly add the icariin alcohol solution to the above solution, stir to make it fully mixed;

[0050] 5) Put the above mixed solution through a microcapsule forming device, drop it into a 4% NaOH aqueous solution to form it, and place it together with the gel bath in a 37°C water bath for 8 hours for in-situ crystallization, and then repeatedly soak it with dei...

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Abstract

The invention discloses chitosan-hydroxylapatite in-situ loaded icariin composite microspheres and a preparation method thereof. The preparation method is characterized in that a micro-capsule forming device is adopted, chitosan is used as an organic substrate, and soluble calcium salt and soluble phosphate are used as precursors of inorganic phase nanometer hydroxylapatite; the in-situ crystal induction effects of hydroxyl radicals and amino radicals in the chitosan on the hydroxylapatite are used for adjusting and controlling the in-site heterogeneous nucleation crystallization of an inorganic mineral on the organic substrate; and meanwhile, a traditional Chinese medicine, namely icariin which has the effect of promoting proliferation and differentiation on osteoblast is compounded, and the chitosan-hydroxylapatite in-situ loaded icariin composite microspheres are prepared through in-situ simulation. The chitosan-hydroxylapatite in-situ loaded icariin composite microspheres are bone filling materials, have good biocompatibility and biodegradability and are expected to serve as repair materials for bone tissue defects to be widely applied in clinic; the preparation processes are simple and easy; and the method is mild.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a chitosan-hydroxyapatite in-situ loaded icariin composite microsphere and a preparation method thereof. Background technique [0002] In recent years, with the deterioration of the ecological environment, the number of patients with bone diseases has increased significantly. Usually bone tissue repair methods include: autologous bone graft repair, allogeneic bone graft repair, and various artificial bone substitute materials such as metal alloys and polymers. Autologous bone is the best implant for bone defect repair, but its supply sources are limited, allogeneic and heterogeneous bone have immune rejection and high sample preparation and processing costs; the most widely used metal material, although it has strong mechanical strength However, its composition is very different from that of natural bone. The metal material cannot chemically bond with th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/12A61L27/20A61L27/54A61L27/50B01J13/02
Inventor 陈景帝张惠张玉珏王自豪张其清
Owner FUZHOU UNIV
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