67 results about "Cellular lipid" patented technology

Methods, vessels, and systems are provided for processing lipids contained in biomass, such as organisms grown in aqueous media or wastes in aqueous media, to produce fatty acid esters as components of a fuel, such as biofuels. The methods described herein are able to efficiently convert cellular lipids to biofuels from lipid-containing biomass such as algae.

A method for determination of at least one of the lipid species in a biological sample comprising subjecting the sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to multidimensional electrospray ionization mass spectrometry using either precursor ion or neutral loss scanning (or both) of all naturally occurring aliphatic chains, lipid fragments and precursor ions leading to observed fragments to generate a multidimensional matrix whose contour densities provides structural and quantitative information directly without chromatography. A method for determination of lipid content and / or lipid molecular species composition and quantity directly from lipid extracts of a biological sample comprising subjecting said lipid extract to electrospray ionization multidimensional mass spectrometry by comparisons to standards and algorithms described herein.

The present invention provides an optimized method of continuously culturing an auxotrophic marine microorganism in a fermentor under aerobic conditions at Y g / l of cell dry matter, CDM, wherein Y is in the range from 100-300 g / l, comprising culturing said auxotrophic marine microorganism in a culture medium comprising a carbon source, gradually added, in an amount of (Y×h) gram per litre of culture broth, wherein h is in the range from 1.1-3.0, and with a residence time of 20-100 h. The method maintains a high productivity of cellular lipids, especially polyenoic acids.

The invention discloses a siRNA interference-based anti-HBV gene-based drug mediated by complex carriers. Based on the characteristic of HBV and expression products thereof, active epitope protein in HBsAg expressed by HBV is biologically linked with cationic liposome and specially binds with hepatic cell surface receptors with the help of the epitope protein in the HBsAg to induce RNAi to enter hepatic cells; meanwhile, the meltability of bilayers of the cationic liposome and the cytolipin is used for increasing the efficiency of an siRNA expressed carrier entering into the hepatic cells. The drug of the invention can improve the effectiveness of the siRNA, biological stability in the human body, safety and effective targeting delivery and can be used in the clinical treatment.

Disclosed is an anti-tumor drug with nor-icariin as the effective ingredient. Pharmacological test effect proves that the nor-icariin has the activity to inhibit the production of cellular lipid, can reduce the production of cellular fatty acid by inhibiting the activity of FAS, has a function of inhibiting the production and causing withering of microvascular endothelial cells, shows a broad-spectrum anti-tumor function in a cytotoxicity test of thirty-three tumor cell strains of a human body, and shows a function in strongly inhibiting the production of tumor, such as prostate cancer cells LnCAP, lung cancer cells NCI-H23 and colon cancer cells HCT-116 of a human body in a tumor transplantation nude mice model test. A pharmacokinetics test of the nor-icariin in the body of a little mouse proves that the nor-icariin can be kept with a certain concentration, good for better anti-humor effect. The acute toxicity test of the nor-icariin in the body of the mouse shows nontoxicity; therefore the nor-icariin is proved to be a newly-developed broad spectrum anti-humor drug with broad development prospect.

A one-step process for the lysis of microalgae cell walls and separation of the cellular lipids for use in biofuel production by utilizing a hydrophilic ionic liquid, 1-butyl-3-methylimidazolium. The hydrophilic ionic liquid both lyses the microalgae cell walls and forms two immiscible layers, one of which consists of the lipid contents of the lysed cells. After mixture of the hydrophilic ionic liquid with a suspension of microalgae cells, gravity causes a hydrophobic lipid phase to move to a top phase where it is removed from the mixture and purified. The hydrophilic ionic liquid is recycled to lyse new microalgae suspensions.

A liposomal composition and a method of using the same for achieving intracellular delivery of a liposome-entrapped agent is described. The liposomes are composed of a pH sensitive lipid and include a targeting ligand to direct the liposomes to a target cell. The liposomes also include a stabilizing component, such a polymer-derivatized lipid, where the polymer is attached to the lipid by a releasable linkage. Administration of the liposomes results in cellular internalization and destabilization of the liposome for intracellular delivery of the entrapped agent.

The invention provides modified DGAT1 proteins that are modified in the N-terminal region upstream of the acyl-Co A binding site. The modified DGAT proteins show enhanced activity, without reduced protein accumulation when expressed in cells. The modified DGAT1 proteins of the invention can be expressed in cells to increase cellular lipid accumulation and / or modify the cellular lipid profile. The invention also provides polynucleotides encoding the modified DGAT1 proteins, cells and compositions comprising the polynucleotides or modified DGAT proteins, and methods using the modified DGAT1 proteins to produce oil.

Disclosed herein are peptides with domains that promote lipid efflux from cells and optionally possess at least one anti-inflammatory domain or a domain that stimulates LCAT activity. Provided herein are methods of using the peptides to treat or inhibit diseases including dyslipidemic disorders, stroke and myocardial infarction. Also provided are methods of detecting plaque in vessels using the labeled peptides of the present invention.

The invention provides a cell lipidomics analysis method based on ultra-high performance liquid chromatography tandem high-resolution mass spectrometry, and relates to the field of analytical chemistry and the field of lipidomics research. The method comprises the following steps: adjusting the density of cells with PBS, and carrying out ultrasonic disruption to obtain a cell suspension; extracting the cell suspension with methanol and a dichloromethane solution to obtain a sample to be detected; separating and determining the sample to be detected through ultra-high performance liquid chromatography-quadrupole rod-electrostatic field orbitrap Fourier transform mass spectrometry; and carrying out qualitative analysis on the measured lipid compound by utilizing software. According to the present invention, the method has characteristics of simple operation and wide lipid coverage, does not require the standard substance, and can be effectively used for the lipidomics analysis of different types of cells so as to further develop the biological effect research on cytotoxicity or activity and the like.

Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by up-regulating diacylglycerol acyltransferase and down-regulating triacylglycerol lipase. In some embodiments, a DGA1 protein is expressed and a native TGL3 gene is knocked out, thereby increasing the synthesis of triacylglycerol and decreasing its consumption, respectively.

A method for determination of at least one of the lipid species in a biological sample comprising subjecting the sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to multidimensional electrospray ionization mass spectrometry using either precursor ion or neutral loss scanning (or both) of all naturally occurring aliphatic chains, lipid fragments and precursor ions leading to observed fragments to generate a multidimensional matrix whose contour densities provides structural and quantitative information directly without chromatography. A method for determination of lipid content and / or lipid molecular species composition and quantity directly from lipid extracts of a biological sample comprising subjecting said lipid extract to electrospray ionization multidimensional mass spectrometry by comparisons to standards and algorithms described herein.

In some embodiments, the present invention provides a method of elevating lipid content in vegetative (non-seed) plant or algae cells, plant tissues, or whole plants by genetically modifying the plant or algae to express a lipid droplet-associated protein or polypeptide (such as fat-specific protein 27) of mammalian origin. Also provided are genetically-modified plant or algae cells, plant tissues, or whole plants with elevated cellular lipid content, expressing a lipid droplet-associated protein or polypeptide (such as fat-specific protein 27) of mammalian (e.g. human) origin.

Disclosed herein are peptides with domains that promote lipid efflux from cells and optionally possess at least one anti-inflammatory domain or a domain that stimulates LCAT activity. Provided herein are methods of using the peptides to treat or inhibit diseases including dyslipidemic disorders, stroke and myocardial infarction. Also provided are methods of detecting plaque in vessels using the labeled peptides of the present invention.

The invention discloses a TRAF4 (Tumor Necrosis Factor Receptor-associated Factor 4) and application of an inhibitor thereof in preparing medicine for treating fatty liver and associated diseases. Bytaking a human normal liver L02 cell line, a TRAF2 knocked-down L02 cell line, primary hepatocyte and TRAF4 knocked-down primary hepatocyte as research objects and stimulating and inducing a liver cell lipid accumulation model by PA (Palmitic Acid) and OA (Oleic Acid), functions of a TRAF4 gene can be researched. The research discovers that when expression of the TRAF4 is reduced, L02 cell lipid accumulation stimulated and induced by the PA and the OA is remarkably reduced, i.e., the TRAF4 gene is capable of promoting occurrence and development of the fatty liver and the associated diseases, so that the TRAF4 provides a target for developing the medicine for preventing, relieving and / or treating the fatty liver and the associated diseases.

The invention discloses a method for detecting the influences of a buccal cigarette product on lipid peroxidation of cells. The method comprises the following steps: (1) preprocessing test samples; (2) preparing a human oral keratinocyte single cell suspension; (3) calculating the cell concentration of the human oral keratinocyte single cell suspension; (4) inoculating the human oral keratinocyte single cell suspension with cells; (5) grouping the test samples; (6) setting the test dosage of the test samples; (7) preparing a test sample culture product; (8) incubating the test samples; (9) collecting test sample incubated products; (10) cracking and centrifuging the test sample incubated products; (11) diluting a standard product; (12) detecting the test samples; and (13) calculating the malondialdehyde content. The effective treatment of the samples, the optimal setting of the detection dosage and the correct selection of the target cells make the method accurately and effectively detect the influences of the buccal cigarette product on the lipid peroxidation of the cells.

Experiment researches find that the expression quantity of GPR31 protein is gradually increased along with the prolonging of tissue ischemia time, and a positively related relationship exists between the expression quantity of GPR31 protein and tissue ischemia reperfusion injury severity. The overexpression of GPR31 can increase the activity of liver cells, cardiac muscle cells and kidney cells caused by H / R treatment. Moreover, the decrease of GPR31 expression in the liver cells can inhibit cellular lipid accumulation caused by palmitate and oleic acid stimulation. The overexpression of GPR31 in the cardiac muscle cells can increase cardiac hypertrophy caused by Angiotensin II. Therefore, the GPR31 inhibitor can be used for preparing drugs, and the drugs can be used for curing related diseases of ischemia reperfusion injury, cardiac hypertrophy and related diseases and liver metabolism diseases.

The invention provides a novel DGAT1 protein with improved properties over known DGAT proteins, particularly known DGAT1 proteins from plants. The novel DGAT1 protein of the invention can be expressed in cells to increase cellular lipid accumulation. Expression of the DGAT1 protein of the invention in cells results in a higher level of lipid than any of several other plant DGAT1 proteins tested by the applicants. The invention provides polynucleotides encoding the novel DGAT1 protein of SEQ ID NO:39, constructs, cells, plant, plant parts and progeny comprising the polynucleotides, and methods of use of the polynucleotides and polypeptides of the invention.

The invention relates to a traditional Chinese medicine composition for repairing ephedrine tissue damage. The traditional Chinese medicine composition comprises the following components in percentage by weight: 40-50% of astragalus, 35-45% of Sichuan lovage rhizome and 10-20% of liquorice. The preparation method of aqueous solution of the traditional Chinese medicine composition comprises the following steps of: (1) respectively taking out equal astragalus, Sichuan lovage rhizome and liquorice, respectively adding 5 times of water, soaking for 1h, decocting for 30min, filtering medicine liquid for standby, respectively adding 5 times of water to the decoction dreg, decocting for 30 minutes, and filtering medicine liquid for standby; (2) taking medicine liquid of the step (1) twice, respectively mixing and concentrating constant volume of the medicine liquid to be 250mL to prepare concentrated liquids; and (3) taking out each concentrated liquid in the step (2), and mixing according to formula proportion. According to the traditional Chinese medicine composition for repairing the ephedrine tissue damage, disclosed by the invention, the anti-oxidation capability of tissue cells can be improved, and peroxidation of cell lipid is suppressed; in addition, by improving the activity of cells, the cell apoptosis is suppressed, the tissue cell damage is released, the tissue cell repair is facilitated, the constitution of a patient is improved, and the certain treatment or withdrawal function is provided for ephedrine-habituated animals.

The invention discloses application of novel loquat leaf sesquiterpene glycoside in relieving liver cell lipid deposition, and also discloses application in preparation of drugs and health-care foodsfor preventing or treating non-alcoholic fatty liver. The name of the sesquiterpene glycoside is nerolidol-3-O-alpha-L-arabinopyranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1-> 2)-[alpha-L-rhamnopyranosyl-(1-> 6)]-beta-D-glucopyranoside.

The invention discloses application of a fructus lycii water decoction in preparing drug for treating retinal tissue injury caused by heroin. The fructus lycii water decoction is a crude drug water decoction containing 0.2-0.4g / mL of crude drug. A great number of studies on fructus lycii find that the fructus lycii water decoction can improve SOD activity of tissue cells, inhibit cell lipid peroxidation and inflammatory reaction of, inhibit iNOS activity of the cells, reduce tissue structure injury, reduce expression of Bax, Caspase-3, TNF-alpha and NF-kappa B and improve cell activity, has anti-oxidation effect, anti-inflammatory effect and retina protecting effect and can effectively retard or relieve injury of retinal tissue caused by heroin. New application is added to the fructus lycii water decoction, and the application is of certain reference significance in developing and utilizing fructus lycii medicinal resources with western characteristics in the future.

The invention discloses a method for purifying main-effect phenolic ingredients inhibiting hepatic cell lipid accumulation in litchi pulp. Four different phenolic ingredients like procyanidine and flavonoid substance in the litchi pulp are separated to realize inhibiting effect of the four phenolic ingredients on oleic-acid-induced lipid accumulation in hepatic cells.

The invention discloses an application of an ethyl acetate extract of emblic leafflower fruits in prevention and treatment of atherosclerosis, and provides an application of the ethyl acetate extractof emblic leafflower fruits in preparation of any one or more of the following products: an in-vivo and in-vitro oxidation inhibitor for low-density lipoprotein; an inhibitor for macrophage and vascular endothelial cell damage caused by oxidized low-density lipoprotein; a macrophage foaming transition inhibitor; a macrophage inflammatory factor secretion inhibitor; a macrophage lipid efflux promoter; an ABCA1 transport protein promoter; and an ABCG1 transport protein promoter. Emblic leafflower fruit resources are rich, so that the ethyl acetate extract of the emblic leafflower fruits is easyto prepare. The extract is developed into food additives, health foods, medicines and other related products with the effect of preventing and treating atherosclerosis, and has very high application value.

The invention discloses an application of a protein signal transduction regulatory factor 14 in the preparation of drugs for treating fatty liver and related diseases, and belongs to new uses of RGS14. A result of the research of the functions of an RGS14 gene through a palmitate (PA) and oleic acid (OA) stimulus-induced liver cell lipid accumulation model with a human normal liver L02 cell line and an RSG14 over-expression L02 cell line as research objects shows that the RSG14 over-expression can significantly improve the lipid accumulation in liver cells, so the RGS14 can protect the liver cells from being subjected to lipoid denaturation. The RGS14 is a body endogenous protein, so the RGS14 has an excellent safety when used as a drug.