Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of ethyl acetate extract of emblic leafflower fruits in prevention and treatment of atherosclerosis

A technology of ethyl acetate extraction and atherosclerosis, which is applied in the field of application of ethyl acetate extract of Phyllanthus emblica in the prevention and treatment of atherosclerosis, can solve the problem of complex pathogenesis and treatment targets, and cannot treat atherosclerosis problems, to achieve the effect of inhibiting lipid deposition and vascular atheromatous plaque formation, blocking the formation of atherosclerosis, and inhibiting the transformation of foam

Inactive Publication Date: 2020-06-19
QILU UNIV OF TECH
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the occurrence of atherosclerosis is caused by multiple pathways, and the pathogenesis and therapeutic targets are relatively complex, emblica fruit juice or crude ethanol extract cannot comprehensively and effectively treat atherosclerosis caused by various pathways

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ethyl acetate extract of emblic leafflower fruits in prevention and treatment of atherosclerosis
  • Application of ethyl acetate extract of emblic leafflower fruits in prevention and treatment of atherosclerosis
  • Application of ethyl acetate extract of emblic leafflower fruits in prevention and treatment of atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The preparation of embodiment 1. Amla emblica ethyl acetate extract

[0068] Such as figure 1 As shown, the traditional Chinese medicine emblica was crushed to a particle size of 20 mesh, and the ratio of liquid to material was 1:10, using a Soxhlet extractor, and sequentially extracted with petroleum ether, chloroform, ethyl acetate, n-butanol, and water. That is, add 100 grams of emblica powder into the extraction chamber of the Soxhlet extractor, add 1000 ml of chloroform into the flask of the Soxhlet extractor, set the oil bath at 70° C., and heat and extract for 180 minutes. Afterwards, when siphon reflux occurred, the heating was stopped and cooled to room temperature. Carefully pour out the chloroform extract, centrifuge at 4000 rpm for 5 minutes at room temperature, and take the supernatant and keep it. Afterwards, 1000 ml of petroleum ether was added into a Soxhlet extractor flask, and the extract was heated at 85° C. for 180 minutes. After siphoning occurs,...

Embodiment 2

[0069] Embodiment 2. Preparation of Amlica emblica ethyl acetate extract

[0070] Such as figure 1 As shown, the traditional Chinese medicine emblica was crushed to a particle size of 20 mesh, and the ratio of liquid to material was 1:10, put into a round bottom flask, added 1000ml of 70% ethanol, and extracted for 4 hours under reflux in a boiling water bath. Afterwards, cool to room temperature, pour out the extract, centrifuge at room temperature at 4000rpm for 5 minutes, and take the supernatant. Rotary evaporation to obtain the extract, which can be directly used or freeze-dried to obtain the ethanol crude extract of Amla emblica (EC). The above crude extract was further purified, that is, the supernatant was sequentially extracted with the same volume of petroleum ether, chloroform, ethyl acetate, and n-butanol. That is, firstly add 1000ml of petroleum ether to 1000ml of ethanol extract, 40 ℃ ultrasonic for 1 hour, stand and separate layers, separate the petroleum ethe...

Embodiment 3

[0071] Embodiment 3. the preparation of emblica extract

[0072] Take the fresh fruit of Amla emblica with core, pulverize it with a traditional Chinese medicine grinder and extract the juice, and control the temperature during the juice extraction process to prevent local overheating. The squeezed fruit juice was extracted with equal volumes of petroleum ether, chloroform, ethyl acetate, n-butanol and pure water according to the extraction method in Example 2. Rotary steamed ethyl acetate extract to obtain extract, used directly or freeze-dried, and weighed. Depending on the purpose, the dry paste or freeze-dried powder was dissolved in physiological saline, cell culture medium or initially dissolved in DMSO and then diluted to a final concentration of DMSO <0.1% for subsequent experiments.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of an ethyl acetate extract of emblic leafflower fruits in prevention and treatment of atherosclerosis, and provides an application of the ethyl acetate extractof emblic leafflower fruits in preparation of any one or more of the following products: an in-vivo and in-vitro oxidation inhibitor for low-density lipoprotein; an inhibitor for macrophage and vascular endothelial cell damage caused by oxidized low-density lipoprotein; a macrophage foaming transition inhibitor; a macrophage inflammatory factor secretion inhibitor; a macrophage lipid efflux promoter; an ABCA1 transport protein promoter; and an ABCG1 transport protein promoter. Emblic leafflower fruit resources are rich, so that the ethyl acetate extract of the emblic leafflower fruits is easyto prepare. The extract is developed into food additives, health foods, medicines and other related products with the effect of preventing and treating atherosclerosis, and has very high application value.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to the application of ethyl acetate extract of emblica emblica in preventing and treating atherosclerosis. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] In recent years, cardiovascular and cerebrovascular diseases have become a major threat to human health and the disease with the highest mortality rate. Its main pathological basis, atherosclerosis, has become the main cause of death in my country. According to the latest statistics from the National Office on Aging, by the end of 2017, China's elderly population over the age of 60 reached 240 mi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/47A61K45/06A61P9/10
CPCA61K36/47A61K45/06A61K2236/31A61K2236/33A61K2236/333A61K2236/55A61P9/10A61K2300/00
Inventor 邢澍刘健敏刘文杰约翰·K·别利茨基周明扬潘学芳张永春
Owner QILU UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com