Method for detecting influences of buccal cigarette product on lipid peroxidation of cells

Abstract

The invention discloses a method for detecting the influences of a buccal cigarette product on lipid peroxidation of cells. The method comprises the following steps: (1) preprocessing test samples; (2) preparing a human oral keratinocyte single cell suspension; (3) calculating the cell concentration of the human oral keratinocyte single cell suspension; (4) inoculating the human oral keratinocyte single cell suspension with cells; (5) grouping the test samples; (6) setting the test dosage of the test samples; (7) preparing a test sample culture product; (8) incubating the test samples; (9) collecting test sample incubated products; (10) cracking and centrifuging the test sample incubated products; (11) diluting a standard product; (12) detecting the test samples; and (13) calculating the malondialdehyde content. The effective treatment of the samples, the optimal setting of the detection dosage and the correct selection of the target cells make the method accurately and effectively detect the influences of the buccal cigarette product on the lipid peroxidation of the cells.

Description

technical field [0001] The invention belongs to the technical field of biological effect evaluation of tobacco products, in particular to a method for detecting the influence of snus products on cell lipid peroxidation. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. Snus products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to turn from traditional cigarette products. According to the components and morphological characteristics of the products, snus products are divided into traditional Snus-type snus products containing tobacco leaf raw materials and new snus products without tobacco leaf raw materials (such as tobacco lozenges). ...

AI Technical Summary

Problems solved by technology

However, there are a large number of primary formulas, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biological test indicators to screen the primary formula of the product to obtain the effect of reducing the adverse feelings of the human body? The product formula is still in the exploratory stage, and there is no uniform standard method

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