Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of protein signal transduction regulatory factor 14 in preparation of drugs for treating fatty liver and related diseases

A technology of signal transduction and regulation factor, which is applied in the field of gene function and application to achieve the effect of high safety

Active Publication Date: 2018-06-19
WUHAN UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of RGS14 in fatty liver has not yet been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of protein signal transduction regulatory factor 14 in preparation of drugs for treating fatty liver and related diseases
  • Application of protein signal transduction regulatory factor 14 in preparation of drugs for treating fatty liver and related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] [Example 1] Effect of overexpression of RGS14 on fat accumulation in liver cells

[0087] Construct a lentiviral expression vector overexpressing RGS14, transfect HEK-293T cells, package lentivirus, infect L02 cells to construct a stable cell line (pHAGE-RGS14) overexpressing RGS14, and overexpress empty vector as a control group (pHAGE group) , Whether the stable cell line highly expresses RGS14 was detected by Western blot; the successfully expressed L02 cell plate was divided into 4 groups, namely the pHAGE control group, the pHAGE-RGS14 control group, the pHAGE experimental group, and the pHAGE-RGS14 experimental group. After the cells adhered to the wall, palmitate (PA, final concentration 0.4mM) and oleic acid (OA, 0.8mM) were added to stimulate the experimental group, while the same amount of BSA was added to the control group, and oil red O staining was performed 16 hours later.

[0088] Western blot detection results such as figure 1 As shown, the protein expr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of a protein signal transduction regulatory factor 14 in the preparation of drugs for treating fatty liver and related diseases, and belongs to new uses of RGS14. A result of the research of the functions of an RGS14 gene through a palmitate (PA) and oleic acid (OA) stimulus-induced liver cell lipid accumulation model with a human normal liver L02 cell line and an RSG14 over-expression L02 cell line as research objects shows that the RSG14 over-expression can significantly improve the lipid accumulation in liver cells, so the RGS14 can protect the liver cells from being subjected to lipoid denaturation. The RGS14 is a body endogenous protein, so the RGS14 has an excellent safety when used as a drug.

Description

technical field [0001] The invention belongs to the field of gene function and application, in particular to the function and application of protein signal transduction regulatory factor 14 (regulator of G protein signaling 14, RGS14) gene in fatty liver and related diseases, and the preparation of RGS14 as the target gene Application in drugs for preventing, alleviating and / or treating fatty liver and related diseases. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a metabolic disease without a history of excessive drinking, characterized by fat accumulation and degeneration in liver cells, and its pathogenic factors include central obesity, insulin resistance, blood lipid abnormalities and metabolic syndrome [1-3] . NAFLD is the most common chronic liver disease. According to global statistics, the incidence of NAFLD is about 25%, and 10-20% of the patients eventually develop into non-alcoholic steatohepatitis (NASH), while About one-third of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/19A61K48/00A61P1/16
CPCA61K38/19A61K48/005
Inventor 李红良胡宇峰
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com