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Method for preparing herba epimedii low-glycoside components from herba epimedii

A technology of low glycoside and epimedium, which is applied in the field of compound extraction and preparation, can solve the problems of failing to ensure complete conversion of polyglycosides, destroying the structure of chemical components, unfavorable environmental protection by waste water, etc., and achieves strong safety and specificity, The effect of high catalytic activity and high conversion rate

Pending Publication Date: 2019-10-18
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Preliminary studies have shown that oral administration of total flavonoids of Epimedium helps transform Epimedium polyglycoside flavonoids into low-glycoside flavonoids through the metabolism of intestinal bacteria, so that it can be better absorbed into the blood and exert its medicinal effect, but it is subject to individual differences and intestinal bacteria. Factors such as group instability cannot promote the stable transformation of polyglycosides into epimedium low-glucosides with better efficacy
Secondly, the study found that the high-temperature processing method can help the polyglycosides in the total flavonoids of Epimedium Herba to break bonds, desugar, and transform into low-glycosides, but the processing process is complicated and the cost is high, and it cannot ensure that the polyglycosides are completely converted into cypress. Goatwort low in glycosides, not conducive to large-scale production
The traditional acid hydrolysis reaction, the reaction is too violent, it is easy to directly hydrolyze and remove all the sugar groups connected to the polyglycoside, and even excessive hydrolysis destroys the structure of the chemical components. Secondly, the operation is complicated and the waste water generated by the reaction is not conducive to environmental protection.

Method used

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  • Method for preparing herba epimedii low-glycoside components from herba epimedii

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Take an appropriate amount of Epimedium decoction pieces, add 15 times the volume of 80% ethanol, heat and extract once, extract for 3 hours, filter and combine the filtrate, recover ethanol under reduced pressure at 50°C, and concentrate the extract to contain Epimedium per ml The quality of Herba Herba Herba Decoction Pieces was 0.1g to obtain the concentrated solution of Epimedium flavone polyglucoside component extract; Add cellulase to the extracted concentrated solution, stir evenly, and adjust the pH of the solution to 4.5, set the stirring speed to 100r / min, heat and react at 40°C for 48 hours, and obtain the enzymatic hydrolysis reaction solution, take an appropriate amount to detect Epimedin A and Epimedin B, epimedin C and icariin basically all disappear, reaching 99% enzymolysis conversion rate; (3) get the enzymolysis reaction liquid and be 10000r / min centrifugation 10min with the rotating speed, discard supernatant, collect precipitation, according to ...

Embodiment 2

[0031](1) Take an appropriate amount of Epimedium decoction pieces, add 25 times the volume of 40% ethanol, heat and extract once, extract for 2 hours, filter and combine the filtrate, recover ethanol under reduced pressure at 60°C, and concentrate the extract to contain Epimedium per ml The quality of Herba Herba Herba Decoction Pieces was 0.05g, and the concentrated solution of Epimedium flavone polyglucosides component was obtained; Add cellulase to the extracted concentrated solution, stir evenly, and adjust the pH of the solution to 6.5, set the stirring speed to 50r / min, heat and react at 50°C for 24 hours, and obtain the enzymatic hydrolysis reaction solution, take an appropriate amount to detect Epimedin A and Epimedin B, epimedin C and icariin basically all disappear, reaching 100% enzymolysis conversion rate; (3) get the enzymolysis reaction solution and be centrifuged at 8000r / min for 20min with a rotating speed, discard the supernatant, collect the precipitate, and ...

Embodiment 3

[0033] (1) Take an appropriate amount of Epimedium decoction pieces, add 20 times the volume of 50% ethanol, heat and extract twice, extract for 1.5 hours, filter and combine the filtrate, recover ethanol under reduced pressure at 55°C, and concentrate the extract until it contains perml The quality of Herba Herba Herba Decoction Pieces is 0.2g, and the concentrated solution of Epimedium flavone polyglucoside fraction is obtained; Add cellulase to the component extraction concentrate, stir evenly, adjust the pH of the solution to 5.5, set the stirring speed to 60r / min, heat and react at 40°C for 36h, and obtain the enzymatic hydrolysis reaction solution, take an appropriate amount to detect epimedin A, epimedin Ding B, epimedin C and icariin basically all disappeared, and the enzymolysis conversion rate reached 99%; (3) take the enzymolysis reaction solution and centrifuge it at a speed of 3000r / min for 40min, discard the supernatant, collect the precipitate, According to the ...

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Abstract

The invention discloses a method for preparing herba epimedii low-glycoside components from herba epimedii. By means of an in-vitro enzymolysis technology, enzymolysis and desugaring are carried out on herba epimedii flavonoid polysaccharide obtained from alcohol extraction herba epimedii decoction pieces, and the herba epimedii low-glycoside components are obtained through conversion. The methodis high in enzymolysis conversion rate, easy and convenient to operate, environmentally friendly, economical and feasible. Polysaccharide substances, such as epmedin A, epmedin B, epmedin C and icariin, in herba epimedii total flavonoids can be completely enzymolyzed and converted into low-glycoside compounds, baohuoside II, sagittatoside A, sagittatoside B, rhamnose-based icarisid II, anhydroicaritin-3-O-rhamnose-dideoxyfuranose and baohuoside I. A stable composition structure ratio is achieved, so that the content of the herba epimedii low-glycoside components is increased.

Description

technical field [0001] The invention relates to a method for extracting and preparing epimedium low-glycoside components from epimedium, and belongs to the technical field of compound extraction and preparation. Background technique [0002] Elderly people are very prone to osteoporosis. my country has gradually entered an aging society, and the number of patients with osteoporosis will also increase. Osteoporosis is the breakdown of the body's bones and can have serious complications. The bone toughness of patients with this disease decreases, and systemic fractures are extremely prone to occur. The incidence rate of people over 60 years old is about 55%, and the incidence rate of fractures reaches 10%. Therefore, it is particularly important to find more efficient drugs for the treatment of osteoporosis. [0003] Epimedium, a traditional Chinese medicine in my country, has the effects of nourishing kidney yang, dispelling rheumatism, and strengthening bones and muscles. ...

Claims

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Application Information

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IPC IPC(8): C12P19/60C07H1/08C07H17/07
CPCC12P19/60C07H1/08C07H17/07
Inventor 贾晓斌封亮
Owner CHINA PHARM UNIV
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