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Rhodococcus ruber and application thereof in degradation of phenol pollutants

A technology of rhodococcus and pollutants, which is applied in the field of rhodococcus and its application in the degradation of phenol pollutants, and can solve the problems that have not yet been applied to rhodococcus

Active Publication Date: 2012-07-25
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no application of Rhodococcus Erythrococcus in degrading pollutants such as phenol

Method used

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  • Rhodococcus ruber and application thereof in degradation of phenol pollutants
  • Rhodococcus ruber and application thereof in degradation of phenol pollutants
  • Rhodococcus ruber and application thereof in degradation of phenol pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: as figure 1 Shown, the degradation performance of phenol of Rhodococcus rubrum SD3;

[0015] Cultivate the preserved Rhodococcus rhodococcus SD3 strain with LB medium, take 10ml of the bacterial solution in the logarithmic phase, centrifuge and separate the bacterial cells, add an equal volume of sterile water to prepare a bacterial suspension, take 1ml of the above bacterial suspension and add 100ml of In the phenol inorganic salt medium (phenol concentration: 1.0 g / L), shake culture at 35° C. and 200 r / min for 72 hours. The degradation rate of phenol was measured by 4-aminoantipyrine spectrophotometry to be 99.73%.

Embodiment 2

[0016] Embodiment 2: as figure 2 Shown, the culture status of Rhodococcus rubella SD3 when different common pollutants were used as the sole carbon source.

[0017] The SD3 strain of Rhodococcus rubrum was picked from the preserved slant, inoculated in LB culture medium, and activated for 28 hours at 35°C and 200 rpm on a shaking table. Take the activated bacterial liquid and inoculate it in the culture medium with isooctane, cyclohexane, benzene, n-heptane, toluene, acetonitrile, chlorobenzene, naphthalene, n-hexane, and 1-naphthol as the only carbon source according to the inoculation amount of 6%. After 72 hours of shaking culture in inorganic salt medium at 35°C and 200r / min shaking table, the dry weight of bacteria reached 0.0203g, 0.0197g, 0.0181g, 0.0180g, 0.0148g, 0.0147g, 0.0136g, 0.0120g, 0.0145g g, 0.0066g.

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Abstract

The invention relates to Rhodococcus ruber and application of Rhodococcus ruber in degradation of phenol pollutants. The Rhodococcus ruber is designated as Rhodococcus ruber SD3, which is preserved in a preservation unit appointed by SIPO (State Intellectual Property Office), wherein the preservation unit is a China Center for Type Culture Collection, the preservation date is February 26, 2012, the accession number is CCTCC NO: M 2012035, and the Latin name is Rhodococcus ruber SD3. The Rhodococcus ruber has the following technical effect: after Rhodococcus ruber SD3 in an inorganic salt medium of phenol (phenol concentration is 1.0g / L) is subjected to shaking table vibration culture at 35 DEG C and 200r / min for 72h, the phenol degradation rate is 99.73%. The Rhodococcus ruber SD3 can also degrade substances such as isooctane, cyclohexane, benzene, n-heptane, methylbenzene, acetonitrile, chlorobenzene, naphthalene, n-hexane, and 1-naphthol.

Description

technical field [0001] The invention relates to a rhodococcus erythrococcus and its application in degrading phenol pollutants. Background technique [0002] Phenol is a raw material or intermediate in petrochemical, papermaking, agriculture, pharmaceutical synthesis and other industries, and is the main pollutant in industrial wastewater. Phenol is an aromatic compound, which is highly toxic to humans, animals and plants and is difficult to degrade. Therefore, my country has included phenol in the blacklist of China's environmental priority pollutants, and has strict regulations on the discharge of phenol-containing wastewater. Regulation. [0003] With people's in-depth understanding of the pollution status of phenol-containing wastewater and its hazards, as well as the continuous development of water treatment technology, people have adopted a variety of phenol treatment technologies. At present, there are many methods for treating phenolic substances in wastewater at ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 彭仁王启明
Owner JIANGXI NORMAL UNIV
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