44 results about "Rhodococcus ruber" patented technology

A Rhodococcus ruber Em (CGMCC No.0868) features that its cells, cell suspension, or immobilized cells can degradate the paraffin and polycyclic arylhydrocarbon because the paraffin and polycyclic arylhydrocarbon are used as its only carbon source and energy source for growing to generate the bioemulsifier of ester. Said emulsifier can obviously decrease the surface tension of aqueous solution, has strong emulsifying power to ester substance, and can increase the solubility of paraffin and polycyclic arylhydrocarbon in water, so it can be used for treating the oil-contained sewage and repairing the petroleum polluted soil.

The invention relates to a bacterial strain of Rhodococcus ruber P14 CGMCC NO.2343. The bacterial strain has the characteristic of floating up from oil matters; the bacterial strain can grow by taking the oil matters as unique carbon source and energy source and degrade the oil matters; and the bacterial strain can grow by taking polycyclic aromatic hydrocarbon as unique carbon source and energy source and degrade hydrocarbon compounds, such as phenanthrene, pyrene, benzopyrene, and the like. The bacterial strain can degrade the oil matters and hydrocarbon compounds, especially the polycyclic aromatic hydrocarbon can be applied in the biological treatment of oily waste water and the biological repair (biological remediation) of oil-contaminated soil.

The invention relates to Rhodococcus ruber and application of Rhodococcus ruber in degradation of phenol pollutants. The Rhodococcus ruber is designated as Rhodococcus ruber SD3, which is preserved in a preservation unit appointed by SIPO (State Intellectual Property Office), wherein the preservation unit is a China Center for Type Culture Collection, the preservation date is February 26, 2012, the accession number is CCTCC NO: M 2012035, and the Latin name is Rhodococcus ruber SD3. The Rhodococcus ruber has the following technical effect: after Rhodococcus ruber SD3 in an inorganic salt medium of phenol (phenol concentration is 1.0g / L) is subjected to shaking table vibration culture at 35 DEG C and 200r / min for 72h, the phenol degradation rate is 99.73%. The Rhodococcus ruber SD3 can also degrade substances such as isooctane, cyclohexane, benzene, n-heptane, methylbenzene, acetonitrile, chlorobenzene, naphthalene, n-hexane, and 1-naphthol.

The invention belongs to the technical field of agricultural biology, and particularly discloses a Rhodococcus ruber XS-2 strain of which the collection number is CGMCC No.11562. The Rhodococcus ruber XS-2 strain has the function of degrading chemical pesticides. The invention also discloses a microbial preparation containing the Rhodococcus ruber and a preparation method thereof. The microbial preparation is compounded from the Rhodococcus ruber, Serratieae Bacillus subtilis and Brevibacillus brevis. The microbial preparation has the functions of restoring pesticide-polluted soil, preventing diseases and promoting growth, has high environmental safety, and has favorable development and application prospects.

The invention relates to an immobilized cell of a mutagenized bacterium M1 of Rhodococcus ruber SD3 and application thereof in degradation of phenol pollutants. For the mutagenized bacterium M1 prepared by mutagenizing Rhodococcus ruber SD3 with 0.3% lithium chloride and screening, the degradation rate on 1.5 g / L phenol within 72 hours is 99.77%. The immobilized cell having a diameter of 6 mm is prepared by embedding the mutagenized bacterium M1 with 1% sodium alginate and 1% polyvinyl alcohol. When the immobilized cell is repeatedly used 5 times, the degradation rate on 2 g / L phenol within 72 hours is more than 98%. Thus, the mutagenized bacterium M1 of Rhodococcus ruber SD3 and the immobilized cell thereof can efficiently degrade phenol pollutants, and can be used for biological treatment of phenol-containing industrial wastewater.

The invention provides a Rhodococcus ruber and application thereof in preparing a decabromodiphenyl oxide degrading agent, relating to application of microbes. The collection number of the Rhodococcus ruber TAW-CT127 is CCTCC NO.M 2015212. The Rhodococcus ruber TAW-CT127 can be used in preparing the decabromodiphenyl oxide degrading agent. The decabromodiphenyl oxide is the unique carbon source in the decabromodiphenyl oxide degradation culture medium. After the Rhodococcus ruber TAW-CT127 is cultured in the degradation culture medium using decabromodiphenyl oxide as the unique carbon source at 28 DEG C under the pH value of 7.4 at the shaking table rotation frequency of 160 rpm, the degradation rate of the Rhodococcus ruber for decabromodiphenyl oxide is up to 91%.

The invention discloses rhodococcus ruber and application of same as an immunologic adjuvant in preparing vaccine. The rhodococcus ruber is also called rhodococcus ruber RDC-01, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 16640. The rhodococcus ruber disclosed by the invention has the function of increasing and regulating the body immunity andis capable of nonspecifically enhancing the activity of TB (Tuberculosis) lymphocyte, macrophagocyte and NK cells and inducing multiple cell factors such as interferon, and the rhodococcus ruber canbe used as the immunologic adjuvant after being inactivated so as to be added in an oil-adjuvant inactive vaccine, so that generation of an animal antibody induced by the vaccine can be obviously promoted; compared with single use of the oil-adjuvant inactive vaccine, a high-titre antibody can be generated, the use is safe, long-term and short-term toxic and side effects are not generated, and anapplication prospect in the field of preparation of vaccines for animals is good.

The invention provides Rhodococcus ruber capable of degrading phthalates and application thereof. The Rhodococcus ruber is YC-YT1 collected under CGMCC No. 13959, is capable of degrading 100 mg / L di(2-ethylhexyl)phthalate in an inorganic salt by 85% within 12 hours, and capable of decreasing 100 mg / L di(2-ethylhexyl)phthalate, dipropyl phthalate and butyl benzyl phthalate in an organic salt medium by 100% within 5 days, and also has good degrading effect for other phthalates. The Rhodococcus ruber YC-YT1 has a wide tolerant range for salt ion concentration, pH and temperature, is applicable to the biological repair of phthalate environmental pollutants and the treatment of industrial wastewater, and has good economic value and promising application prospect.

The invention belongs to the field of microorganisms and discloses a biological agent for treating ammonia-nitrogen-containing wastewater. The biological agent comprises rhodococcus ruber, micrococcus luteus, enterococcus faecalis, acinetobacter baumannii, arthrobacter crystallopoietes, thiobacillus denitrificans and pseudomonas fluorescens. The biological agent comprises multiple strains, has the advantages of reasonable compatibility and strong synergic effect and can be used for effectively removing ammonia nitrogen, sulfide, aniline substances and industrial COD in wastewater.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

The invention discloses Rhodococcus ruber PTA-2, which is classified and named Rhodococcus ruber PTA-2, is preserved in the China General Microbiological Culture Collection Center on February 9, 2015, and has the preservation number of CGMCC NO.10538. According to the present invention, the Rhodococcus ruber PTA-2 can grow by adopting p-toluenesulfonic acid as the sole carbon source, wherein the degradation rate on the 500 mg / L p-toluenesulfonic acid is 96%; the immobilized pellets prepared from the Rhodococcus ruber PTA-2 provides the good p-toluenesulfonic acid degradation effect, wherein the degradation rate on the 500 mg / L p-toluenesulfonic acid is more than 90%; and the production cost is low, the use is convenient, and the Rhodococcus ruber PTA-2 is suitable for promotion and application in the p-toluenesulfonic acid-containing wastewater treatment.

The present invention relates to a hydroxysteroid dehydrogenase and an application thereof in synthesis of an ursodeoxycholic acid precursor, and specifically discloses a 12-alpha-hydroxysteroid dehydrogenase derived from rhodococcus ruber and a mutant thereof, encoding gene and amino acid sequences, a recombinant expression vector containing the gene, a recombinant expression transformant, and anapplication of the hydroxysteroid dehydrogenase catalyzing oxidation of 12-alpha-hydroxysteroids to form 12-carbonyl steroids. Compared with the prior art, a used recombinant steroid dehydrogenase-catalyzed oxidation reaction is NAD+coenzyme-dependent type. The hydroxysteroid dehydrogenase has advantages of low application cost, simple operation, mild reaction conditions, environmental friendliness, high yield, etc., and thus has a very good prospect in an application of cholic acid as a raw material for production and preparation of the ursodeoxycholic acid precursor.

The invention relates to a special rhodococcus ruber (CGMCC NO. 17012) fermentation method, a preparation process of adjuvants with different dosage forms and an application as an adjuvant in animalvaccines. Rhodococcus ruber strains are separated from a farm and obtained through single colony cloning purification and identification, the invention provides a special fermentation process where the strain inactivation product is used as an animal vaccine adjuvant, and provides a preparation process of different dosage forms of adjuvants containing the strain and the product. Animal experimentsprove that when the adjuvant product is used for univalent or multivalent animal vaccines, particularly Newcastle disease inactivated vaccine, avian influenza inactivated vaccine and swine fever livevaccine, definite non-specific immune enhancement effect can be provided, specifically, the antibody peak value level induced by the animal vaccine is obviously improved, the time for producing the protective antibody level is advanced, the antibody maintenance period is prolonged, and the immune effect of the animal vaccines is enhanced.

The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and / or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and / or reducing ketones.

The invention provides a rhodococcus ruber strain, a rhodococcus ruber bacterium preparation and an application of thalli cells and extracts of rhodococcus ruber. The strain is rhodococcus ruber (Rhodococcus ruber) FIM-12 which is preserved in the China General Microbiological Culture Collection Center on April 3, 2019, with the preservation number being CGMCC No.17525. The strain can be used forpreparing rhodococcus ruber cells and extracts of the rhodococcus ruber through microorganism fermentation: after the rhodococcus ruber (Rhodococcus ruber) FIM-12 is subjected to fermentation and culture, thalli cells are collected, the thalli cells are washed with purified water so that wet cells are obtained, the wet cells are subjected to repeated freeze-thawing combined with ultrasonic wall breaking, then extraction with anhydrous alcohol is performed, and the rhodococcus ruber extracts are obtained. The rhodococcus ruber strain can be applied to industrialized fermentation and production,the rhodococcus ruber thalli cells and the rhodococcus ruber extracts are prepared, the prepared products have the functions of increasing immunity activity and restraining effects, and the rhodococcus ruber strain can be used as a production strain for further research and development.

The invention discloses an engineering bacteria with high tolerance to acrylamide and acrylonitrile for producing nitrile hydratase, a construction method for the engineering bacteria and application of the engineering bacteria in producing the acrylamide by a microorganism method, and belongs to the technical field of industrial microbiology. A bacterial strain for producing the acrylamide from the nitrile hydratase with high yield is used as a parent strain; the parent strain additionally expresses mutational RNA polymerase sigma factor gene in the engineering bacterium; and the engineering bacteria are Rhodococcus ruber TH-5(amdA-) / pNV18AM CGMCC No.3725 preferably. The tolerance of the engineering bacteria to the acrylonitrile and the acrylamide is increased by 1 time compared with that of the parent strain; and the half-life period of the nitrile hydratase is prolonged by 1.3 times when the engineering bacteria are immersed in 40 percent solution of acrylamide. The invention also discloses the construction method for the engineering bacteria and the application of the enginnering bacteria in producing the acrylamide. In the invention, the acrylamide is produced from the bacterial strain, the concentration of a batch hydration product is high, reaction batches are increased, and concentration load is reduced, so that the production cost is reduced obviously.

The invention discloses a pyridine degradation strain a5 which is identified as Rhodococcus ruber and has been preserved in China General Microbiological Culture Collection Center, wherein the preservation date is August 31, 2016 and the preservation number is CGMCC No.12919. The invention also discloses an application of the pyridine degradation strain a5 in producing pyridine degradation fungicide. The degradation fungicide can degrade pyridine and can achieve the degradation rate 90% or above of the pyridine remained in soil within a short period of time and the degradation rate 95% or above of the pyridine remained in high-concentration pyridine wastewater. The degradation fungicide provided by the invention can be applied to the general fermentation equipment in fermentation industry for production and has the advantages of low production cost, convenience in use and good removing effect.

The invention discloses rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds. The rhodococcus ruber HDRR1 is assigned the accession number CCTCC M20191008, and deposited with the China Center for Type Culture Collection, Wuhan, China on December 4, 2019. The strain HDRR1 has strong purifying capacity on the inorganic nitrogen and phosphorus in aquaculture tail water, is good in environmental adaptability and has no adverse effects on farmed fish and shrimp. Applications of the rhodococcus ruber HDRR1 on purifying the inorganic nitrogen and phosphorus in the aquaculture tail water of seawater ponds are also disclosed.

The invention belongs to the field of treatment of wastewater in food enterprises and in particular relates to an essence production wastewater treating agent and also relates to an essence production wastewater treatment method. The essence production wastewater treating agent comprises the following components in parts by weight: a physical water purifying agent, a compound microbial agent and an enzymic preparation, wherein the physical water purifying agent comprises 1-5 parts of activated carbon, 1-5 parts of organically modified zeolite and 1-5 parts of polyacrylamide; the compound microbial agent comprises 0.02-0.1 part of paracoccus aminovorans powder, 0.05-0.2 part of rhodococcus ruber powder, 0.05-0.2 part of sulfate reducing bacteria powder and 0.04-0.2 part of moraxella powder; and the enzymic preparation comprises 0.05-0.1 part of xylanase, 0.04-0.09 part of lignan hydroxylase and 0.01-0.06 part of laccase. By adopting the wastewater treating agent and the wastewater treatment method, complex large-particle organic matters difficult to degrade are hydrolyzed into simple organic matters easy to degrade, thus greatly reducing the content of SS (suspended substance) in the wastewater, and the treated wastewater has high BOD (biochemical oxygen demand) and COD (chemical oxygen demand) removal rates and reaches the specified discharge standard.

The invention discovers Rhodococcus ruber 4.1187 which can be used for efficiently catalytically preparing optically pure (S)-(+)-2-phenylpropionic acid. The invention has the advantages of mild reaction conditions and environment-friendliness. The prepared optically pure (S)-(+)-2-phenylpropionic acid is an important 2-arylpropionic acid non-steroidal anti-inflammatory drug or an important chiral building block. The chemical formulas of (5)-(+)-2-phenylpropionic acid and (R)-(-)-2-phenyl-propionamide are shown in the specification.

The invention discloses rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water. The rhodococcus ruber HDRR2Y has a preservation number of CCTCC M20191009, the preservation date is December 4, 2019, the preservation name is Rhodococcus ruber HDRR2Y, the preservation authority is China Center for Type Culture Collection, and the preservation address is Wuhan, China. The strain HDRR2Y has relatively strong removing capability on inorganic nitrogen and phosphorus in aquaculture water or tail water, is good in environmental adaptability, and hasno adverse effect on cultured fishes and shrimps. The invention further discloses an application of the rhodococcus ruber HDRR2Y in removing inorganic nitrogen and phosphorus in seawater pond culturetail water.

The invention relates to a preparation method of 2,6-difluorobenzamide by utilizing rhodococcus ruber. The method comprises the following steps: (1) fermentation and culture of seeds; (2) preparation of rhodococcus ruber suspension fluid; (3) conversion of 2,6-difluorobenzonitrile; (4) preparation of finished products. The invention provides a preparation method of 2,6-difluorobenzamide by utilizing rhodococcus ruber to convert 2,6-difluorobenzonitrile into 2,6-difluorobenzamide. The microorganism conversion method has a very high catalytic efficiency, increases the substrate conversion rate, under a material concentration of 3.5 mol / L, the 2,6-difluorobentrinile conversion rate is as high as 100%, the 2,6-difluorobenamide selectivity is 100%, and no by product 2,6- difluorobenzoic acid is generated. The separation and purification technology of target product is simplified, and the method has the advantages of mild reaction conditions, low requirements on equipment, and easy application to production.

The invention discloses Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide (DMAC) and degradation application of the Rhodococcus ruber HJM-8. The Rhodococcus ruber HJM-8 is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postal code is 430072, the preservation number is CCTCC NO: M 2021654, and the preservation date is June 1, 2021. According to the rhodococcus ruber HJM-8 with the DMAC degradation performance, the degradation rate of DMAC with the initial concentration being 500 mg.L <-1> can reach 100% within 24 h, and the discovery of the degradation bacterium has important significance on efficient purification of industrial wastewater containing DMAC.

Isolated Rhodococcus ruber, a product derived from the Rhodococcus ruber (in particular, a product derived from the Rhodococcus ruber cell wall), a method for preparing the product derived from the Rhodococcus ruber, and use of the product derived from the Rhodococcus ruber in prevention and / or treatment of lichen planus. The product derived from the Rhodococcus ruber effectively reduce the erosion area of lichen planus and relieve pain.

The invention relates to Rhodococcus ruber and application of Rhodococcus ruber in degradation of phenol pollutants. The Rhodococcus ruber is designated as Rhodococcus ruber SD3, which is preserved in a preservation unit appointed by SIPO (State Intellectual Property Office), wherein the preservation unit is a China Center for Type Culture Collection, the preservation date is February 26, 2012, the accession number is CCTCC NO: M 2012035, and the Latin name is Rhodococcus ruber SD3. The Rhodococcus ruber has the following technical effect: after Rhodococcus ruber SD3 in an inorganic salt medium of phenol (phenol concentration is 1.0g / L) is subjected to shaking table vibration culture at 35 DEG C and 200r / min for 72h, the phenol degradation rate is 99.73%. The Rhodococcus ruber SD3 can also degrade substances such as isooctane, cyclohexane, benzene, n-heptane, methylbenzene, acetonitrile, chlorobenzene, naphthalene, n-hexane, and 1-naphthol.

The invention relates to a bacterial strain of Rhodococcus ruber P14 CGMCC NO.2343. The bacterial strain has the characteristic of floating up from oil matters; the bacterial strain can grow by taking the oil matters as unique carbon source and energy source and degrade the oil matters; and the bacterial strain can grow by taking polycyclic aromatic hydrocarbon as unique carbon source and energy source and degrade hydrocarbon compounds, such as phenanthrene, pyrene, benzopyrene, and the like. The bacterial strain can degrade the oil matters and hydrocarbon compounds, especially the polycyclicaromatic hydrocarbon can be applied in the biological treatment of oily waste water and the biological repair (biological remediation) of oil-contaminated soil.

The invention discloses a new strain Rhodococcus ruber JJ-3, and application of the Rhodococcus ruber JJJ-3 in degradation of acrylic acid, wherein the Rhodococcus ruber JJ-3 is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postal code is 430072, the preservation number is CCTCC NO: M 2019546, and the preservation date is July 12, 2019. The invention provides Rhodococcus ruber JJ-3 with acrylic acid degradation performance, and application in degradation of acrylic acid wastewater, wherein the strain has a degradation rate of 100% onacrylic acid with an initial concentration of 1000 mg.L<-1> within 16 h, and the discovery of the degrading bacterium is of great significance to efficient purification of acrylic acid in industrial wastewater.