Rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water and application of rhodococcus ruber HDRR2Y
A technology of Rhodococcus rhodochrous and inorganic nitrogen, which is applied in the field of Rhodococcus rhodochrous HDRR2Y, can solve the problems of difficulty in separating and purifying nitrifying bacteria and denitrifying bacteria, staying on a small scale, failing to achieve the effect of large-scale industrial application of directional cultivation of single microorganisms, etc. Good environmental adaptability, reduced water body replacement, and remarkable removal effect
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Embodiment 1
[0029] Example 1 Screening and cultivation of Rhodococcus rhodococcus HDRR2Y for purifying seawater pond culture tail water inorganic nitrogen and phosphorus
[0030] 1. Material preparation
[0031] 1.1. Bacterial source
[0032] In the intensive prawn farming area of Touwei Village, Ganggang Town, Huidong, Guangdong, the tail water samples from 60-90 days of cultivation were collected and separated and cultured on selective medium plates.
[0033] 1.2. Medium
[0034] (1) Selective liquid medium: CH 3 COONa: 1g, yeast extract: 1g, MgSO 4 ·7H 2 O: 0.4g, NaCl: 0.1g, CaCl 2 2H 2 O: 0.05g, NaHCO 3 : 0.3g, KH 2 PO 4 : 1g, 1mL of trace element solution, the above drugs were dissolved in distilled water, and fixed to 1000mL, pH7.0.
[0035] Trace element solution: EDTA: 2.5g, ZnSO 4 ·7H 2 O: 10.95g, MnSO 4 ·H 2 O: 1.54g, CuS0 4 ·5H 2 O: 0.39g, CoCl 2 ·6H 2 O: 0.2g, FeSO 4 ·7H 2O: 7g, glutamic acid: 0.02g, the above drugs were respectively dissolved in distille...
Embodiment 2
[0041] Example 2 Identification of the screened Rhodococcus rhodococcus HDRR2Y
[0042] The present invention carried out 16S rDNA molecular identification on the screened Rhodococcus rubrum HDRR2Y, and determined the species of the strain from the molecular level, combined with analysis of bacterial morphological characteristics and physiological and biochemical characteristics. 16S rDNA sequence analysis mainly follows the following steps:
[0043] 1. Extraction of bacterial genomic DNA:
[0044] (1) Use a sterile toothpick to pick a single colony and inoculate it in the expansion medium for cultivation;
[0045] (2) Take 1.5 mL of bacterial culture solution, centrifuge at 10,000 rpm (11,500×g) for 1 minute, and suck up the supernatant as much as possible;
[0046] (3) Add 200 μL of buffer GA to the cell pellet, shake until the cell is completely suspended, add 180 μL of lysozyme with a final concentration of 20 mg / mL, and treat at 37°C for more than 30 minutes;
[0047] ...
Embodiment 3
[0068] Example 3 Effect of Rhodococcus Erythrococcus HDRR2Y on Inorganic Nitrogen and Phosphorus Removal from Seawater Pond Culture Tail Water
[0069] 1. Growth of the strain
[0070] The bacterial strain Rhodococcus rubrum HDRR2Y that embodiment 1 obtains presses initial concentration 10 4 ~10 6 CFU / mL was inoculated into the tail water of sterilized intensive pond culture, and the strain could enter the plateau stage after about 2 days of growth, and the bacterial count reached 5.1×10 8 CFU / mL, remained stable at 6.3×10 on day 4 8 CFU / mL, the growth curve of Rhodococcus rhodococcus HDRR2Y is as follows figure 1 shown in .
[0071] 2. Inorganic nitrogen and phosphorus removal effect of strains in different salinity aquaculture tail waters
[0072] The tail water of the sterilized prawn intensive culture pond (water salinity 25) was used as the basic test water control, and Rhodococcus rhodococcus HDRR2Y was not added during the test. The bacterium-adding group adjusts ...
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