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Pyridine degradation strain a5, fungicide made from pyridine degradation strain a5 and application thereof

A technology for degrading bacteria and pyridine, which is applied in the field of microbial remediation of environmental pollution, to achieve low production costs, protection of the ecological environment, and good removal effects

Inactive Publication Date: 2017-01-11
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is rarely used in actual bioremediation with high results. The present invention hopes to isolate and identify highly efficient pyridine-degrading bacteria, explore the species relationship of highly efficient pyridine-degrading bacteria, and provide theoretical support for bioremediation of pyridine-contaminated soil.

Method used

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  • Pyridine degradation strain a5, fungicide made from pyridine degradation strain a5 and application thereof
  • Pyridine degradation strain a5, fungicide made from pyridine degradation strain a5 and application thereof
  • Pyridine degradation strain a5, fungicide made from pyridine degradation strain a5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Isolation and identification of strain a5:

[0061] The enrichment matrix used to enrich pyridine-degrading strains was taken from the activated sludge of the pharmaceutical factory wastewater biochemical treatment pool of Wanbei Pharmaceutical Co., Ltd. in Anhui Province. 5.0 g of the activated sludge was added to the basic salt medium, and 50 mg· L -1 pyridine, 30°C, 150rpm cultured for 7 days, transferred to the same medium with 5% inoculum size, transferred continuously for 3 times, and diluted the enrichment solution in gradient, taking 10 -4 -10 -7 Each 0.1mL of the diluted enrichment solution was applied to the 100mg·L -1 On the solid basal salt medium plate of pyridine, after culturing at 30°C for 3 days, pick the grown single colony and inoculate it in a medium containing 200 mg·L -1 In the pyridine basal salt medium, culture at 30°C and 180rpm for 3 days to verify the degradation effect of the degrading bacteria solution.

[0062] Whether pyridine is degra...

Embodiment 2

[0068] Analysis of the growth of strain a5 with different salt concentrations:

[0069] Take 5 clean test tubes and add 2mL LB culture solution respectively, with salt concentration of 0%, 1%, 2%, 3%, and 4%, to explore the effect of different salt concentrations on the growth of pyridine-degrading bacteria a5. The culture conditions were 30°C, 150r / min constant temperature shaker culture, and after 24 hours of culture, the optical density of the culture solution at 600nm was measured with a spectrophotometer. The result is as Figure 4 It is shown that a5 can grow when the salt concentration is 0-1%, and increasing the salt concentration in this range has little effect on the growth of bacteria, and it reaches the highest peak at 1%. But with the further increase of salt concentration, the growth of a5 showed a downward trend. When the salt concentration reached 4%, no bacteria grew.

[0070] Analysis of the growth of different pH strain a5:

[0071] Take 7 clean test tube...

Embodiment 3

[0075] Preparation of degrading bacterial agent of bacterial strain a5

[0076] 1) Inoculate the test tube solution of the degrading strain a5 to the logarithmic phase with an inoculation amount of 0.5% volume ratio in 100mL fermentation medium, shake and culture to the logarithmic phase, and obtain the fermentation strain;

[0077] 2) inoculate 500 liters of seeds with 70% (based on the volume of the fermenter, the same below) of the 500 liters of seeds with the inoculation amount of 5% (v / v, based on the volume of the culture medium) of the above-mentioned fermented strains Cultivate in the culture medium of tank, cultivate to logarithmic growth phase, make seed liquid;

[0078] 3) The inoculation amount of the seed solution is 9% (v / v, based on the volume of the medium, the same below) into the culture medium of the production tank with a liquid volume of 70%.

[0079] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of sterile air...

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Abstract

The invention discloses a pyridine degradation strain a5 which is identified as Rhodococcus ruber and has been preserved in China General Microbiological Culture Collection Center, wherein the preservation date is August 31, 2016 and the preservation number is CGMCC No.12919. The invention also discloses an application of the pyridine degradation strain a5 in producing pyridine degradation fungicide. The degradation fungicide can degrade pyridine and can achieve the degradation rate 90% or above of the pyridine remained in soil within a short period of time and the degradation rate 95% or above of the pyridine remained in high-concentration pyridine wastewater. The degradation fungicide provided by the invention can be applied to the general fermentation equipment in fermentation industry for production and has the advantages of low production cost, convenience in use and good removing effect.

Description

technical field [0001] The invention relates to the restoration of environmental pollution microorganisms, in particular to a pyridine-degrading bacterial strain and its production and application. Background technique [0002] The rapid stage of industrialization has led to the mass use of various synthetic chemicals, which are considered xenobiotics, leading to environmental pollution. At present, industrial production produces and discharges a large amount of aromatic compounds every day, and about 2 / 3 of them are heterocyclic compounds. Heterocyclic compounds are widely used in industrial solvents, dyes, explosives, medicine and pesticides and other fields. Pyridine is one of the typical nitrogen-containing heterocyclic compounds. Pyridine, a nitrogen-containing heterocyclic compound, can be regarded as a compound in which one (CH) of the benzene molecule is replaced by N, so it is also called nitrogen benzene, a colorless or slightly yellow liquid with a foul smell. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 刘洪明姚律成朱韩朱国萍
Owner ANHUI NORMAL UNIV
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