Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in aquaculture tail water of seawater ponds and applications of rhodococcus ruber HDRR1
A technology of Rhodococcus rubrum and inorganic nitrogen, applied in Rhodococcus rubrum HDRR1 and its application fields, can solve the problem of difficult separation and purification of nitrifying bacteria and denitrifying bacteria, small-scale retention, and large-scale industrial application effect of directional cultivation of single microorganisms, etc. problems, achieve good environmental adaptability, reduce water body replacement, and achieve remarkable removal effects
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Embodiment 1
[0029] Example 1 Screening and cultivation of Rhodococcus rhodococcus HDRR1 for purification of inorganic nitrogen and phosphorus in seawater pond culture tail water
[0030] 1. Material preparation
[0031] 1.1. Bacterial source
[0032] In the intensive prawn culture pond in Pinghai Town, Huidong County, Guangdong Province, the tail water samples from 60-90 days of culture were collected, and the selective medium plate was used for separation and culture.
[0033] 1.2. Medium
[0034] (1) Selective liquid medium: CH 3 COONa: 1g, yeast extract: 1g, MgSO 4 ·7H 2 O: 0.4g, NaCl: 0.1g, CaCl 2 2H 2 O: 0.05g, NaHCO 3 : 0.3g, KH 2 PO 4 : 1g, 1mL of trace element solution, the above drugs were dissolved in distilled water, and fixed to 1000mL, pH7.0.
[0035] Trace element solution: EDTA: 2.5g, ZnSO 4 ·7H 2 O: 10.95g, MnSO 4 ·H 2 O: 1.54g, CuS0 4 ·5H 2 O: 0.39g, CoCl 2 ·6H 2 O: 0.2g, FeSO 4 ·7H 2 O: 7g, glutamic acid: 0.02g, the above drugs were respectively disso...
Embodiment 2
[0041] Example 2 Identification of the screened Rhodococcus rubrum HDRR1
[0042] In the present invention, the 16S rDNA molecular identification of the screened Rhodococcus rubrum HDRR1 is carried out, and the species of the strain is determined from the molecular level, combined with the analysis of the morphological characteristics of the bacteria and the physiological and biochemical characteristics. 16S rDNA sequence analysis mainly follows the following steps:
[0043] 1. Extraction of bacterial genomic DNA:
[0044] (1) Use a sterile toothpick to pick a single colony and inoculate it in the expansion medium for cultivation;
[0045] (2) Take 1.5 mL of bacterial culture solution, centrifuge at 10,000 rpm (11,500×g) for 1 minute, and suck up the supernatant as much as possible;
[0046] (3) Add 200 μL of buffer GA to the cell pellet, shake until the cell is completely suspended, add 180 μL of lysozyme with a final concentration of 20 mg / mL, and treat at 37°C for more th...
Embodiment 3
[0068] Example 3 Removal effect of Rhodococcus erythrococcus HDRR1 on inorganic nitrogen and phosphorus in seawater pond culture tail water
[0069] 1. Growth of the strain
[0070] The bacterial strain Rhodococcus rubrum HDRR1 that embodiment 1 obtains presses initial concentration 10 4 ~10 6 CFU / mL is inoculated into the tail water of sterilized intensive culture ponds, and the bacterial count can increase to 10 in about 2 days. 8 CFU / mL, and the bacterial count remained stable at 10 within 3 to 10 days. 8 CFU / mL quantity level, the growth curve of Rhodococcus rhodococcus HDRR1 is as follows figure 1 shown in .
[0071] 2. Inorganic nitrogen and phosphorus removal effect of strains in different salinity aquaculture tail waters
[0072] The tail water of the sterilized shrimp intensive culture pond (water salinity 25) was used as the basic test water control, and Rhodococcus rubrum HDRR1 was not added during the test. The bacterium-added group adjusts the salinity of th...
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Abstract
Description
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Application Information
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