33 results about "Baohuoside I" patented technology

The invention provides a preparation method of baohuoside I, which has a high efficiency and a high conversion rate. The method is characterized in that baohuoside I is obtained by treating a certain concentration of ethanol as a reaction medium, and carrying out enzyme hydrolysis on icariin. The method which adopts a low concentration of an ethanol solution as the reaction medium has the advantages of increase of the specific surface area of a contact reaction of icariin with an enzyme, shortening of the reaction time, improvement of the conversion efficiency and the conversion rate, simple and feasible technology, good purposiveness, no pollution, and realization of industrial production, and overcomes disadvantages of complex operation, much time consumption, low conversion rate, bad purposiveness, difficult separation and purification, and the like existing in the prior art.

The invention discloses a method for preparing herba epimedii low-glycoside components from herba epimedii. By means of an in-vitro enzymolysis technology, enzymolysis and desugaring are carried out on herba epimedii flavonoid polysaccharide obtained from alcohol extraction herba epimedii decoction pieces, and the herba epimedii low-glycoside components are obtained through conversion. The methodis high in enzymolysis conversion rate, easy and convenient to operate, environmentally friendly, economical and feasible. Polysaccharide substances, such as epmedin A, epmedin B, epmedin C and icariin, in herba epimedii total flavonoids can be completely enzymolyzed and converted into low-glycoside compounds, baohuoside II, sagittatoside A, sagittatoside B, rhamnose-based icarisid II, anhydroicaritin-3-O-rhamnose-dideoxyfuranose and baohuoside I. A stable composition structure ratio is achieved, so that the content of the herba epimedii low-glycoside components is increased.

The invention provides a method of preparing Baohuoside I from epimedium medical materials efficiently.

The invention discloses a composition for resisting ageing and improving male energy. The composition is prepared from a raw material and an auxiliary material; the raw material is prepared from the following components in parts by weight: 1-15 parts of nicotinamide mononucleotide, 1-10 parts of protopanoxadiol, 1-9 parts of icarisid I, 2-8 parts of baohuoside I, 3-7 parts of dehydroepiandrosterone and 2-10 parts of ursodesoxycholic acid. The composition is reasonable in composing prescription and proper in compatibility; in the composition, the four of the nicotinamide mononucleotide (NMN), the icarisid I, the baohuoside I and protopanoxadiol are used as monarch drugs together, the composition is capable of comprehensively regulating gonad axis and invigorating kidney-yang and liver, candelay ageing period of the gonad axis and improve the sexual function after being taken for a long time; through intercoordination and synergistic effect of various components, the composition is comprehensive in nutrition, has very good palatability, is particularly suitable for males to take and thus relieving the symptoms of male ageing syndrome and improving the male energy. The invention alsoprovides a preparation containing the composition and a preparation method of the preparation. The preparation method is simple, is moderate in condition and is suitable for industrial batch production.

The invention discloses application of beta-glucosidase to converting total flavonoids of herba epimedii to prepare baohuoside I. According to a method, the specific beta-glucosidase is utilized for converting the total flavonoids of herba epimedii in an oriented mode, so that multiple ingredients rich in the total flavonoids of herba epimedii are all converted into the baohuoside I. The beta-glucosidase obtained through screening can be used for effective enzymolysis of icariin, epimedin A and sagittatoside A rich in the total flavonoids of herba epimedii in preparing the baohuoside I; and meanwhile, epimedin B and sagittatoside B can also be specifically used for preparing the baohuoside I. The beta-glucosidase is low in application cost, and only one type of enzyme is needed; and the converting efficiency of the total flavonoids is high, and the molar conversion ratio is greater than 95%. Meanwhile, the pharmacological activity of the prepared baohuoside I in the aspect of an inhibiting effect on proliferation of tumors such as breast cancer, liver cancer, colon cancer, lung cancer and the like is remarkably higher than that of other main icariin in the total flavonoids of herba epimedii.

The invention relates to a method for obtaining baohuoside I by double-phase enzyme hydrolysis and belongs to the technical field of medicines. An extraction process adopted by the method takes icariin as a raw material, and the baohuoside I is obtained by a double-phase solvent system composed of a buffering solution containing an enzyme and ethyl acetate; in the double-phase enzyme hydrolysis method provided by the invention, the enzyme hydrolysis of the icariin and extraction of the baohuoside I are combined to form one step; the icariin is subjected to the enzyme hydrolysis to generate the baohuoside I, and the baohuoside I is transferred to an ethyl acetate layer from a buffering solution layer, so that hydrolysis reaction can be effectively promoted; the method is a method which has high hydrolysis rate and extraction rate and is simple and convenient to operate.

The preparation method provides a baohuoside-I preparation method which comprises the following steps: carrying out enzymolysis reaction of icariin under the action of pectinase to obtain crude baohuoside-I, and then carrying out two times of crystallization to obtain pure baohuoside-I. The mass yield of the baohuoside-I obtained by the method can reach more than 40% relative to 90% icariin, and the purity is above 99%.

The invention relates to a content determination method used for sinew and bone injury spray, and specifically relates to a content determination method for seven effective constituents, such as paeoniflorin, icariin, ellagic acid, Bepimedin A, Bepimedin B, Bepimedin C and Baohuoside I, in the sinew and bone injury spray. The method comprises preparation of test solution, preparation of referencesolution and HPLC determination. With the content determination method provided by the invention, various effective constituents can be determined simultaneously, a detection line is stable, degree ofseparation is high, stability is excellent, detection accuracy is high, content determination time is reduced, and detection efficiency is improved; in order to control quality of the sinew and boneinjury spray, a relatively comprehensive quantitative detection method is provided, meanwhile, for inspecting stability of the sinew and bone injury spray, a method of stability quantitative inspection for relatively comprehensive effective constituents is provided.

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.

The invention relates to a Baohuoside I dry powder inhalant as well as a preparation method and application thereof. The inhalant provided by the invention is prepared from Baohuoside I utilized as an active substance and pharmaceutically acceptable auxiliary materials. Compared with oral administration, the dry powder inhalant developed according to the technical scheme provided by the invention has the advantage that the treatment effect of the Baohuoside I on tumors of lung is greatly improved.

The invention discloses a method for simultaneously extracting icariin and baohuoside I and II from herba epimedii. The method comprises the following steps of: crushing herba epimedii plant and then passing the crushed herba epimedii plant through a screen mesh, adding alcohol liquor, heating for reflux extraction, and filtering to obtain filtrate B, concentrating under reduced pressure to recycle alcohol to obtain initial extract; extracting the initial extract by pure water, regulating PH, stewing and carrying out suction filtration to obtain icariin extract; carrying out elution on the icariin extract by adopting macroporous adsorption resin, carrying out elution by using alcohol and collecting eluant; adding medical active carbon into the eluant, heating and refluxing, cooling, and filtering to obtain filtrate C; separating, purifying and crystallizing the filtrate C by simulated moving bed chromatography to obtain herba epimedii crystal, and collecting filtrate D; drying the herba epimedii crystal to obtain a pure icariin finished product; adding tween into the filtrate D, carrying out enzymolysis by glucosaccharase or cellulose, and separating and purifying the enzymatic hydrolysate by a chromatographic column mixed and filled by the macroporous resin to obtain products baohuoside I and II.

The invention provides a medicinal composition of baohuoside I, which can increase the solubility of baohuoside I in water, and significantly improve the oral absorption of baohuoside I.

The invention discloses a male health-care composition. The male health-care composition comprises raw materials and auxiliary materials, wherein the raw materials comprise the following components inparts by weight of 1-10 parts of nicotinamide adenine dinucleotide (NADH), 1-10 parts of protopanoxadiol, 2-8 parts of Icarisid I, 3-10 parts of Baohuoside I, 2-9 parts of dehydroepiandrosterone and1-10 parts of ursodeoxycholic acid. The composition is reasonable in formula and compounding ratio and proper in compatibility; the NADH, the protopanoxadiol(PPD), the Icarisid I and the Baohuoside Iare in synergistic reaction, so that the composition can comprehensively regulate sexual gland axis, nourish kidney Yang and nourish the liver, and after being taken for a long term, the composition can delay the ageing cycle of the sexual gland axis and improve the sexual functions. The composition conforms to the theory of traditional Chinese medicine and pharmacy and modern medicine, is convenient to use, is good in absorption effects, is free from bad effects and toxic and side effects, has good palatability, is particularly suitable for males to take, and can relieve or improve various symptoms of male ageing syndromes. The invention further provides a preparation containing the composition, and a preparation technology. A preparation method is simple and mild in conditions, and is suitable for industrialized batch production.

The invention belongs to the field of traditional Chinese medicine compositions, and particularly relates to a detecting method of a traditional Chinese medicine composition for treating rheumatoid arthritis. The traditional Chinese medicine composition for treating rheumatoid arthritis is composed of Tripterygium hypoglaucum, herba epimedii, semen cuscutae and fructus lycii. According to the detecting method of a traditional Chinese medicine composition for treating rheumatoid arthritis, 10 flavonoid compounds comprising hyperoside, caohuoside A, caohuoside C, caohuoside B, icariin, luteolin,quercetin, nobiletin, kaempferol and Baohuoside-I can be simultaneously detected by using a multi-evaluation method; and the hyperoside is taken as an internal referenc, so that the product quality of Tripterygium hypoglaucum, herba epimedii, semen cuscutae and fructus lycii is effectively controlled; the detecting method is simple and convenient, and lays a foundation for better ensuring the clinical curative effect of the traditional Chinese medicine composition for treating rheumatoid arthritis, and also provides a reference for the detection of traditional Chinese medicines rich in flavonoids.

The invention discloses a medical application of an enzymatic hydrolysate of icariin and a main component baohuoside I of the icariin, and particularly relates to an application of the enzymatic hydrolysate of icariin and the main component baohuoside I of the icariin in preparing a medicine for treating skeletal muscle atrophy. In-vitro and in-vivo experiments prove that the enzymatic hydrolysateof icariin or baohuoside I can significantly reduce the skeletal muscle atrophy degree, including increase of muscle fiber cross-sectional area, gastrocnemius muscle wet weight / body mass ratio and aerobic exercise ability, and can up-regulate skeletal muscle protein content by regulating an autophagy system, thus having great application prospects.

The invention discloses an application of baohuoside I in preparation of medicines for preventing and treating alzheimer disease. The baohuoside I is adopted for preparing medicines for treating the alzheimer disease. A series of animal experiments verify that the prepared medicinal composition is capable of preventing and treating learning and memory decline due to the alzheimer disease, reducing formation of characteristic pathologic changed senile plaque due to the alzheimer disease and relieving neuroinflammation response in attack of alzheimer disease. The material in the application is wide in source and easily available, is used for easily and feasibly preparing medicines, and has a good treatment effect.

The invention discloses a method for simultaneously extracting icariin and baohuoside I and II from herba epimedii. The method comprises the following steps of: crushing herba epimedii plant and then passing the crushed herba epimedii plant through a screen mesh, adding alcohol liquor, heating for reflux extraction, and filtering to obtain filtrate B, concentrating under reduced pressure to recycle alcohol to obtain initial extract; extracting the initial extract by pure water, regulating PH, stewing and carrying out suction filtration to obtain icariin extract; carrying out elution on the icariin extract by adopting macroporous adsorption resin, carrying out elution by using alcohol and collecting eluant; adding medical active carbon into the eluant, heating and refluxing, cooling, and filtering to obtain filtrate C; separating, purifying and crystallizing the filtrate C by simulated moving bed chromatography to obtain herba epimedii crystal, and collecting filtrate D; drying the herba epimedii crystal to obtain a pure icariin finished product; adding tween into the filtrate D, carrying out enzymolysis by glucosaccharase or cellulose, and separating and purifying the enzymatic hydrolysate by a chromatographic column mixed and filled by the macroporous resin to obtain products baohuoside I and II.

The invention relates to a method for obtaining baohuoside I by double-phase enzyme hydrolysis and belongs to the technical field of medicines. An extraction process adopted by the method takes icariin as a raw material, and the baohuoside I is obtained by a double-phase solvent system composed of a buffering solution containing an enzyme and ethyl acetate; in the double-phase enzyme hydrolysis method provided by the invention, the enzyme hydrolysis of the icariin and extraction of the baohuoside I are combined to form one step; the icariin is subjected to the enzyme hydrolysis to generate the baohuoside I, and the baohuoside I is transferred to an ethyl acetate layer from a buffering solution layer, so that hydrolysis reaction can be effectively promoted; the method is a method which has high hydrolysis rate and extraction rate and is simple and convenient to operate.

The preparation method provides a baohuoside-I preparation method which comprises the following steps: carrying out enzymolysis reaction of icariin under the action of pectinase to obtain crude baohuoside-I, and then carrying out two times of crystallization to obtain pure baohuoside-I. The mass yield of the baohuoside-I obtained by the method can reach more than 40% relative to 90% icariin, and the purity is above 99%.

The invention discloses a baohuoside I tablet, which is prepared from the following raw materials in parts by weight: 6-30 parts of baohuoside I, 55-86 parts of diluents, 1.5-2.5 parts of adhesives, 1.5-7 parts of disintegrants, 0.5-5 parts of lubricants, and 0.1-6 parts of surfactants. The method for preparing the baohuoside I tablet comprises the following steps: weighing, mixing, crushing, pelletizing, drying, granulating, blending and tabletting. The baohuoside I tablet has the beneficial effects that the baohuoside I tablet disclosed by the invention has the advantages of being easy to disintegrate in water, high in dissolution rate, even to disperse and convenient to take, and the preparation method has the characteristics of being simple in process, convenient to operate, and low in production cost.

The invention belongs to the field of application of anti-tumor immune drugs, and particularly relates to application of a flavonoid USP22 inhibitor in preparation of anti-tumor immune drugs. The skeleton of the flavonoid compound USP22 inhibitor is as described in the specification. The flavonoid compound USP22 inhibitor is baohuoside I, morusin or chrysin. The inhibitor can selectively inhibit USP22, and has a good anti-tumor immune effect.

The invention discloses application of beta-glucosidase to converting total flavonoids of herba epimedii to prepare baohuoside I. According to a method, the specific beta-glucosidase is utilized for converting the total flavonoids of herba epimedii in an oriented mode, so that multiple ingredients rich in the total flavonoids of herba epimedii are all converted into the baohuoside I. The beta-glucosidase obtained through screening can be used for effective enzymolysis of icariin, epimedin A and sagittatoside A rich in the total flavonoids of herba epimedii in preparing the baohuoside I; and meanwhile, epimedin B and sagittatoside B can also be specifically used for preparing the baohuoside I. The beta-glucosidase is low in application cost, and only one type of enzyme is needed; and the converting efficiency of the total flavonoids is high, and the molar conversion ratio is greater than 95%. Meanwhile, the pharmacological activity of the prepared baohuoside I in the aspect of an inhibiting effect on proliferation of tumors such as breast cancer, liver cancer, colon cancer, lung cancer and the like is remarkably higher than that of other main icariin in the total flavonoids of herba epimedii.

The invention relates to the technical field of traditional Chinese medicines, in particular to application of baohuoside I in preparation of a medicine for treating rheumatoid arthritis and osteoporosis, and further relates to the medicine for treating the rheumatoid arthritis and the osteoporosis, the active ingredient of the medicine comprises baohuoside I, and the concentration of the baohuoside I in the medicine is 0.5-5 mu M. The baohuoside I inhibits osteoclast differentiation by inhibiting an Nf-kb signal channel and regulating an autophagy system, and is used for treating rheumatoid arthritis and osteoporosis. As a traditional Chinese medicine monomer, the invention develops a new application for the baohuoside I preparation, has important significance for treating rheumatoid arthritis and osteoporosis, and provides a new thought for promoting bone formation and inhibiting clinical treatment of joint inflammation.

The invention relates to a quality evaluation method of a five-ingredient capsule for warming and activating collaterals and eliminating arthralgia. 15 ingredients including coumarin, baicalin, epimedin C, icariin, cinnamic acid, wogonoside, cinnamyl aldehyde, baicalein, cinnamyl alcohol, 2-methoxycinnamyl aldehyde, wogonin, baohuoside I, dehydrated icariin, germacrone and pachymic acid are selected as quality markers of the compound; full-wavelength scanning detection is performed, and UHPLC quality evaluation is performed on WWCC (Wuwei Wuntong arthralgia-eliminating capsule) under two wavelengths of 203nm and 270nm; after the analysis method is established and verified, the 15 quality markers in the WWCC sample are quantitatively analyzed, a WWCC fingerprint spectrum under 270nm is constructed, and the overall quality evaluation of the WWCC sample is completed. The method provides a unique idea for WWCC comprehensive quality evaluation, and provides reference and demonstration effects for traditional Chinese medicine compound overall quality evaluation.

The invention provides a Baohuoside-I phospholipid complex and its preparation method. The Baohuoside-I phospholipid complex can be used to remarkably improve pharmaceutical properties of Baohuoside-I.

The invention belongs to the field of pharmacy, relates to a novel medical application of baohuoside I, and specifically relates to an application of baohuoside I in the preparation of drugs for treating asthma, especially drugs for treating allergic asthma. The results of in-vivo animal experiments and in-vitro CD4+T cell culture experiments show that baohuoside I can prominently reduce the bronchial hyper-responsiveness of asthmatic mice, inhibit the airway inflammation, reduce the number of inflammation cells in a bronchoalveolar lavage fluid, inhibit the secretion of chemotactic factors, promote the differentiation of Th1 cells and Treg cells, inhibit IL-17A, and promote the secretion of IFN-gamma and IL-10. The provided baohuoside I can be used as a single component for the preparation of drugs for treating asthma, and is further used to treat asthma, especially allergic asthma.

A quality evaluation method for Wuwei Wentong Chubi Capsules, selecting coumarin, baicalin, epimedin C, icariin, cinnamic acid, wogonoside, cinnamaldehyde, baicalein, cinnamyl alcohol, 2‑methoxy cinnamaldehyde, wogonin, baohuoside I, dehydrated icariin, gemacharone, and pachynic acid, a total of 15 components were used as the quality markers of the compound; full-wavelength scanning detection was carried out, and two kinds of components at 203nm and 270nm were selected. The UHPLC quality evaluation of WWCC (Wuwei Wentong Chubi Capsules) was carried out under the wavelength; after the establishment and verification of the analysis method, 15 quality markers in the WWCC samples were quantitatively analyzed, and WWCC fingerprints were constructed at 270nm. Overall quality evaluation of WWCC samples. This method provides a unique idea for the comprehensive quality evaluation of WWCC, and provides a reference and demonstration role for the overall quality evaluation of traditional Chinese medicine compounds.

The invention provides a processing method for simultaneously improving the resource utilization rate and quality of a herba epimedii medicinal material. The method comprises the following steps: removing impurities and residual stems from harvested fresh herba epimedii leaves, flatly laying the herba epimedii leaves in an electric-heating constant-temperature blast drying oven, drying the herba epimedii leaves at 120-135 DEG C for 30-50 minutes, taking out the herba epimedii leaves, and cooling the herba epimedii leaves. Compared with the prior art and a traditional drying method, the drying processing method provided by the invention has the advantages that the quality of the herba epimedii medicinal material can be remarkably improved, the contents of main components, namely icariin and baohuoside I in herba epimedii are greatly increased and reach the standard of the 2015 edition of Chinese pharmacopoeia, and meanwhile, the contents of other main active components are hardly reduced; moreover, the current situation that the quality of commercially available herba epimedii is generally unqualified can be improved, so abandoned medicinal materials which cannot meet the pharmacopoeia standard can be reused, the utilization rate of the resource of the herba epimedii medicinal material is greatly increased, waste of the resource of the herba epimedii medicinal material is avoided, and considerable economic benefits can be brought to the traditional Chinese medicinal material industry.

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.