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125results about How to "Efficient hydrolysis" patented technology

Method of high-efficiency pretreatment on biomass

The invention discloses a method capable of carrying out green high-efficiency hydrolysis pretreatment on lignocellulose biomass. The method in the invention comprises the steps of hydrolyzing the hemicellulose in the cellulose biomass into saccharide products mainly containing xylose and oligose thereof using a two-step temperature-changing segmentation hydrolysis method so as to accomplish the pretreatment, and adding the pretreated solid raw material mainly containing cellulose and lignin to cellulase so as to accomplish enzymatic hydrolysis and recover the saccharide products to the utmost extent. The invention uses high-temperature liquid water as reaction medium, has the advantages that the method has low cost and is environment-friendly and green. The reaction conditions are moderate, the saccharide products are degraded less, and the recovery rate is high. By adopting the two-step temperature-changing segmentation hydrolysis method, the method not only hydrolyzes the hemicellulose to the utmost extent to obtain the saccharide products, but also further pre-treats the raw material such that the cellulose is made into glucose through enzymatic hydrolysis, reaching the purpose of protecting environment and hydrolyzing the lignocellulose biomass highly effectively. The raw material is capable of realizing waste utilization and promotes the development of green industries.
Owner:GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI

Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material

The invention relates to a method for producing ethanol or acetone and butanol by taking lignocellulose as a raw material. According to the invention, a wet disc grinding machine-ultrafine grain melting and crushing machine physical method is adopted for pretreating the lignocellulose raw material, so that a cellulose structure wrapped by hemicellulose is opened and a cellulose crystalline structure is simultaneously opened, the method can be used for replacing all acid, alkali and gas explosion treatment methods, the conditions of high temperature and high pressure are not required, no external metal or organic matter such as sulfur is added, the pollution is avoided and the hydrolysis efficiency is high. By adopting the method, no secondary substance inhibiting the growth and the fermentation of yeast, bacteria and anaerobic bacteria is generated, and cellulose, the hemicellulose and lignin are not damaged.
Owner:SHANDONG UNIV +1

Preparation of glycosyl microsphere catalyst and process for hydrolyzing cellulose

The invention provides a preparation of a glycosyl microsphere catalyst and a process for hydrolyzing cellulose, belonging to the field of conversion and utilization of a biomass resource and energy resources. The glycosyl microsphere catalyst provided by the invention has a molecular formula of CH0.61O0.57S0.05. The preparation conditions of the catalyst is relatively moderate and the catalyst has a very high catalytic activity; the yield of reducing sugar obtained by hydrolyzing the cellulose in a novel green solvent ion solution is high, and the temperature for the reaction is low and the reaction time is short. Compared with a liquid acid, the reaction process has no pollution and no corrosion, and the catalyst can be circularly utilized for a plurality of times. The process for hydrolyzing the cellulose is a green and effective process. Saccharides for preparing the catalyst are obtained from the natural world and have the advantages of reproducibility, no damages to human health and environment, cheap price and easiness in obtaining. The hydrolysate reducing sugar can be further converted into important platform compounds such as ethanol, 5-hydroxymethylfurfural and the like, and compounds such as bio-fuel and the like, which are capable of replacing traditional petrochemical resources.
Owner:NANKAI UNIV

Enzyme composition for high-efficiency decomposition of cellulose

The invention relates to an enzyme composition for the high-efficiency decomposition of cellulose, which is a recombinant biologic enzyme capable of effectively decomposing the cellulose under the conditions of high temperature and strong acid. The enzyme composition comprises two enzymes from pyrococcus horikoshii and one auxiliary protein, namely, 1, internally cutting 1,4 glucanase, 2, externally cutting beta-glycuronide and 3, forward folding element protein; the proportion of the three proteins in the enzyme composition is preferably 1:1:0.1-1. The enzyme gene of an amino acid residue sequence of the former two enzymes after being analyzed by deletion and optimized and the other two alpha and beta subgroup genes containing the auxiliary protein (the forward folding element protein) are respectively regulated and controlled by a heat promoter of a temperature sensitive type and are expressed with high efficiency in bacteria; the auxiliary protein has the function of greatly enhancing the activity of the two enzymes; therefore, the cellulose enzyme composition can decompose the cellulose with high efficiency into monosaccharide under the conditions of high temperature and strong acid; and the potential application of the cellulose enzyme composition is the decomposition of cellulose class wheat straws for providing a raw material for the production of biologic petroleum.
Owner:郑荣

Preparation method of compound enzyme preparation for promoting production of methane from kitchen waste through anaerobic fermentation

The invention discloses a preparation method of a compound enzyme preparation for promoting production of methane from kitchen waste through anaerobic fermentation. The preparation method is characterized by comprising the following steps of: 1, preparing formula compositions which comprises liquifying enzyme, saccharifying enzyme, cellulase and lipase; 2, separation: classifying and recovering non-biomass parts in the kitchen waste through a manual or mechanical method, wherein a biomass part enters into a composite enzymatic hydrolysis process; 3, crushing and homogenating: crushing the biomass part obtained after the kitchen waste is separated into paste, and homogenating for later use; 4, composite enzymatic hydrolysis; and 5, anaerobic fermentation. According to the invention, from the aspects of methane anaerobic fermentation microbial metabolism and enzyme activity change rule, through using a compound enzyme preparation formula, the effects of promoting rapid and efficient hydrolysis of the kitchen waste and growth and metabolism of methane fermentation microorganism are achieved, and the purpose of efficient and stable operation of a system for producing methane by the kitchen waste through anaerobic fermentation is further realized.
Owner:INST OF PLASMA PHYSICS CHINESE ACAD OF SCI +1

Raw starch-digesting glucoamylase, preparation method thereof and application of raw starch-digesting glucoamylase to raw starch hydrolysis and preparation of ethanol by simultaneous saccharification and fermentation of raw starch

The invention discloses a preparation method of raw starch-digesting glucoamylase and application of the raw starch-digesting glucoamylase to raw starch hydrolysis and preparation of ethanol by simultaneous saccharification and fermentation of raw starch. The raw starch-digesting glucoamylase is obtained after codon optimization, and a nucleotide sequence of the raw starch-digesting glucoamylase is shown as any of from A1) to A5): A1), sequence 3 in a sequence table; A2), nucleotides from 1st position to 1878th position in the sequence 3 in the sequence table; A3), DNA molecules shown by nucleotides in 19th position to 1878th position in the sequence 3 in the sequence table; A4), DNA molecules shown by nucleotides in 19th position to 1857th position in the sequence 3 in the sequence table; A5), a protein sequence shown in sequence 4 in a coding sequence table and showing 98% identity with the sequence limited by A1), A2), A3) or A4). Through experiments, it is proven that raw starch-digesting glucoamylase genes can be effectively expressed in a heterologous expression system to obtain the raw starch-digesting glucoamylase rPoGA15A, and the raw starch-digesting glucoamylase rPoGA15A and alpha-amylase act synergistically to hydrolyze the raw starch rapidly and efficiently and prepare the ethanol by simultaneous saccharification and fermentation of the raw starch.
Owner:GUANGXI UNIV

Fermentation preparation and application of Chaetomium globosum dextranase

The invention discloses fermentation preparation and application of Chaetomium globosum dextranase and belongs to the technical fields of fermentation technology, enzymic preparations and sugar engineering. A high-yield Chaetomium globosum dextranase fermentation method is determined by optimizing medium components and fermentation conditions through orthogonal test, thereby improving the yield ofChaetomium globosum dextranase up to 698.22 U / mL; when applied to hydrolyzing high-molecular-weight glucan and at a final concentration of 2 U / ml, the Chaetomium globosum dextranase can achieve a high-molecular-weight glucan (T2000) hydrolyzing rate up to 97.9% within 15 minutes; the rate of inhibition of the Chaetomium globosum dextranase on biological membranes formed by streptococcus mutans can reach 97.9%, and the clearance rate of the biological membranes reaches up to 49.07%. The prepared Chaetomium globosum dextranase has a good application prospect in food and sugar industries and thefield of medicine.
Owner:JIANGNAN UNIV

Catechol 2,3-dioxygenase gene and application thereof

The invention belongs to the field of genetic engineering and specifically relates to a catechol 2,3-dioxygenase gene and an application thereof. The nucleotide sequence of the catechol 2,3-dioxygenase gene is shown as SEQ ID NO.1. The gene expression product catechol 2,3-dioxygenase is capable of effectively hydrolyzing aromatic compound catechol, 4-methyl catechol, catechol, 1,2,4-pyrogallol and2,6-dimethyl-1,3,4-pyrogallol. The catechol 2,3-dioxygenase gene can be applied to the industries of biological remediation for environmental pollution, industrial wastewater treatment and 2-hydroxylfurfural production, not only can solve the environmental pollution of catechol aromatic compounds, but also can acquire great economic benefit.
Owner:HENAN UNIV OF SCI & TECH

Organic-inorganic hybridized molybdenum arsenate crystal as well as preparation method and application thereof

The invention provides an organic-inorganic hybridized molybdenum arsenate crystal as well as a preparation method and application thereof. The invention relates to the organic-inorganic hybridized molybdenum arsenate crystal as well as the preparation method and the application thereof, aiming at solving the problems that existing reaction conditions for preparing ethylene glycol through hydrolyzing ethylene carbonate are harsh, pollution is easily caused and a catalyst is not easy to recycle. The preparation method comprises the following steps: mixing (NH4)6Mo7O24.4H2O, C10N2H8, NaAsO2 andCuCl2.2H2O; adding a mixture into distilled water and stirring; sealing and putting into a baking oven for heating; cooling at room temperature; finally, washing with distilled water and drying at room temperature to prepare a product. The organic-inorganic hybridized molybdenum arsenate crystal is used as the catalyst and is used for an ester hydrolysis process under normal pressure; the catalystis easy to separate. The crystal prepared by the preparation method can be used as the catalyst and is used for catalyzing ester hydrolysis; ester is not adsorbed and efficient hydrolysis of the ester under the normal pressure is realized. The organic-inorganic hybridized molybdenum arsenate crystal is applied to the field of preparation of the catalyst.
Owner:HEILONGJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for enzymatic degumming of high-acid-value rice bran oil

The invention discloses a method for enzymatic degumming of high-acid-value rice bran oil, and belongs to the technical field of enzyme separation and application. The method comprises the following steps: pretreating high-acid-value rice bran oil by using Lipase G50, and performing enzymatic degumming by using phospholipase; the Lipase G50 (only can act on monoglyceride and diglyceride, and can not act on triglyceride) is used for specifically removing partial glyceride with an emulsifying effect in the high-acid-value rice bran oil; therefore, the wrapped phospholipid in the high-acid-valuerice bran oil is released, the contact probability of phospholipase and phospholipid in the high-acid-value rice bran oil during degumming is increased, the enzymatic degumming reaction rate and efficiency are greatly improved, and the bottleneck problems of low enzymatic degumming rate and low efficiency of the high-acid-value rice bran oil are solved. Meanwhile, after the Lipase G50 is used forpretreating the high-acid-value rice bran oil, the Lipase G50 is enriched in a lower-layer water phase and can be recycled for multiple times.
Owner:SHAANXI UNIV OF SCI & TECH
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