30 results about "Geobacillus thermodenitrificans" patented technology

The invention discloses a recombinant xylosidase XynB1 which is genically encoded and expressed in colon bacillus by expression vector built by xylanase XynB1 obtained through Geobacillus thermodenitrificans NG80-2 genome DNA by means of PCR amplification. The optimal reaction temperature of the xylosidase is 60 DEG C, the optimal reaction PH is 5.6, and the xylosidase has good thermal stability in acid environment. The xylosidase is suitable for decomposing oligomerization xylan to generate xylose.

The invention discloses a recombinant xylanase which is genically encoded and expressed in colon bacillus by expression vector built by xylanase XynA2 obtained through Geobacillus thermodenitrificans NG80-2 genome DNA by means of PCR amplification. The optimal reaction temperature of the xylanase is 70 DEG C, the optimal reaction PH is 9.0, and the xylanase has good thermal stability in alkaline environment. The xylanase is suitable for decomposing xylan in hemicellulose to produce functional oligomerization xylan.

The invention discloses a thermophilic bacterium compound strain obtained by mixing a Ureibacillus sp. strain, a thermophilic geobacillus sp. strain, a geobacillus thermodenitrificans strain and a rhodothermus marinus strain. The strain is obtained from the five strains through activation, culture, compounding and optimization. The compound strain has the advantages of short production cycle, large number of living bacteria and long preservation period, can resist the composting environment at a temperature higher than or equal to 60 DEG C and the like; inactivation of active microorganisms in the compound strain during ultrahigh-temperature aerobic composting can be avoided; the compound strain has higher adaptability and more stable performance than a single thermophilic inoculant, thereby having broad application prospect in rapid and harmless composting of organic waste.

The invention discloses a xylanase XynA1 which is obtained by the PCR amplification of Geobacillus thermodenitrificans NG80-2 genome DNA and a recombinant xylanase for constructing an expression vector to express the genetic code in Escherichia coil. The optimal reaction temperature of the xylanase is 70 DEG C, the optimum reaction pH is 7.6, and the thermal stability is good in neutral and weak alkaline environments. The xylanase is applicable to decomposing xylan in hemicellulose to produce functional xylooligosaccharides.

The invention belongs to the technical field of plant growth-promoting bacteria development, and concretely relates togeobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity. The geobacillus thermodenitrificans TB62 is named as Geobacillus thermodenitrificans in taxonomy, is preserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms in January 05, 2017, and has the preservation No. CGMCC 13530. The geobacillus thermodenitrficans TB62 provided by the invention is firstly screened and separated from samples during a compost high temperature period, and is high in temperature resistant, fast in growth and propagation, and capable of producing lignocellulose-degrading enzyme; a microbial agent containing geobacillus thermodenitrificans TB62 can be beneficial to improving the compost temperature, accelerating organic matter degradation and water-dissolved organic matter (DOM) degradation, improving the content of Kjeldahl nitrogen, and promoting compost maturity; the geobacillus thermodenitrificans TB62 also has a function of biosurfactant production so as to be applicable for preparing a surface active agent.

The invention provides a strain of Geobacillus thermodenitrificans as well as the screening method and the uses thereof. The strain was deposited as the number CGMCC-1228 in the China General Microbiological Culture Collection Center of the China Committee of Culture Collection for Microorganisms. The strain was obtained by primary screening, re-screening, inoculation, domestication and propagation using the bacteria in the aqueous samples from the strata of oil field as the original screening strain. The strain screened in the invention belongs to the genus Geobacillus, which can tolerate a high temperature, and has good thermostability, which can be applied to the industrial production which need the condition of thermostable enzymes, such as fermentation, etc. With its ability of growing well in the oil-reservoir environment, degrading alkanes, decreasing viscosity and increasing fluidity of crude oil, the strain is able to remarkably enhance the yield of oil and improve the transporting efficiency of oil. Additionally, with its good ability of degrading oil, it can be developed for the treatment and clearing of the material such as the oil contaminated water, etc., so it can be useful in protection of the environment. This strain also has the ability of decreasing the surface tension of substances, which can be applied to the industry of biosurfactants preparation.

The invention discloses a recombinant xylosidase which is genically encoded and expressed in colon bacillus by expression vector built by xylanase XynB2 obtained through Geobacillus thermodenitrificans NG80-2 genome DNA by means of PCR amplification. The optimal reaction temperature of the xylosidase is 65 DEG C, the optimal reaction PH is 6.0, and the xylosidase has good thermal stability in acid environment. The xylosidase is suitable for decomposing oligomerization xylan to generate xylose.

The invention discloses a decomposed solid fermentation fungicide using agricultural organic wastes such as cow dung and straws (walnut pruning branches and cotton stalks) as raw materials, includingfive bacteria of saccharomyces cerevisiae, bacillus methylotrophicus, geobacillus thermodenitrificans, bacillus simplex and lactobacillus parabuchneri. The invention also discloses a method for preparing the decomposed solid fermentation fungicide. The active fungicide utilizes the synergistic effect between the five bacteria in the composting materials to rapidly form a dominant flora and fully exerts the deodorization and decomposing process promoting effects. At the same time, the problem that the effective utilization rate of the agricultural organic wastes is not high, the natural fermentation composting time is long, and the product quality is poor is solved. A fertilizer subjected to decomposed fermentation can improve the soil fertility of the farmland, regulate the pH of the soil,and has good ecological and economic benefits.

The invention discloses a method of adopting biotechnology to produce admiralty fuel oil, taking oil slurry as raw materials. According to the method, microbial agent composed of activated thermophilic denitriding bacillus NG80-2 (Geobacillus thermodenitrificans) and Mycobacterium goodiisp at the ratio of 1:1 is reacted with high-kinematic viscosity oil slurry in an inorganic salt solution to obtain the No. 380 admiralty fuel oil. A reaction system comprises 1-5%t of activated mixed agents, 50% of oil slurry and the balance of inorganic salt solution. The production method has the advantages of simple process, simplicity in equipment, low cost and environment protection, can produce fuel oil with good quality, and has better processing effect for the oil slurry; and the produced oil product can completely meet the standard of the No. 380 admiralty fuel oil, and has better application prospect.

The present invention relates to a recombinant GRAS (Generally Recognized As Safe) strains expressing thermophilic arabinose isomerase as an active form and method of food grade tagatose by using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Geobacillus stearothermophilus DSM22 and Geobacillus thermodenitrificans, a recombinant expression vector containing the gene, a recombinant GRAS strains expressing the thermophilic arabinose isomerase as an active form by transformed with the expression vector, and a method of preparing food grade tagatose from galactose by using the same.

The invention belongs to the field of microorganisms, and discloses Geobacillus thermodenitrificans obtained by artificial mutagenesis and application thereof in cellulose degradation. The applicant separates a strain of Geobacillus thermoslucanase with the highest cellulase activity from a sample of pig carcass and straw compost in a high-temperature period, and obtains a strain of Geobacillus thermodenitrificans with high cellulase yield after high-temperature domestication and ultraviolet mutagenesis. The preservation number of the strain is CCTCC NO: M2020324. The Geobacillus thermodenitrificans BGSC95A1 with cellulase activity is reported for the first time. A plurality of carbon sources can be utilized, the Geobacillus thermodenitrificans has a degradation effect on a plurality of celluloses, and the cellulase concentration in fermentation liquor produced by fermentation is high.

The invention belongs to the technical field of petroleum extraction, and particularly relates to a biological enzyme composite preparation for petroleum extraction as well as a preparation method andapplication of the composite preparation. The composite preparation comprises the following components, in percentages by mass: 1-2% of a functional microbial fermentation liquid, 5-10% of a nutritional agent, 0.5-1.0% of a biological enzyme, and the rest is water, wherein the functional microbial fermentation liquid is a fermentation liquid of thermotoga or geobacillus thermodenitrificans; the nutritional agent is composed of a nitrogen source and a phosphorus source, the nitrogen source is one of ammonium chloride, urea and sodium nitrate, and the phosphorus source is one of dipotassium hydrogen phosphate, disodium hydrogen phosphate and diammonium hydrogen phosphate; and the biological enzyme is composed of acetylxylan esterases and alkylsuccinate synthase. The composite preparation has the characteristics of a simple construction technology, high operability and a good field test effect, the effective period of an oil well is more than 50 months, the average daily oil increase ofa single well is more than 10 tons, and the input-output ratio is greater than 1:10.

The invention relates to a geobacillus thermodenitrificans DC8 strain and application thereof, the geobacillus thermodenitrificans DC8 strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2021, and the preservation number is CGMCC No.23318. The strain provided by the invention has the capability of degrading excess sludge to extract protein in a high-temperature environment, and the protein extraction rate can reach 29.7%. Protein in sludge can be effectively utilized by utilizing the strain, the reduction and degradation effect of residual sludge is improved, and the strain has a good application prospect. According to the invention, an efficient bacterium source is provided for the reduction of the residual sludge of a sewage plant, the functional application of the geobacillus thermodenitrificans is widened, and the geobacillus thermodenitrificans has a relatively high practical value.

The invention discloses geobacillus thermodenitrificans engineering bacteria capable of efficiently degrading nitroalkanes. The geobacillus thermodenitrificans engineering bacteria capable of efficiently degrading the nitroalkanes are obtained by using an NOEs (nitroalkane-oxidizing enzymes) gene that overexpresses a nitroalkane degradation pathway. The modified engineering bacteria are named NG-S1 and NG-S2. The nucleotide sequence of the NOEs gene is represented by SEQ ID NO. 1-2. Compared with wild-type geobacillus thermodenitrificans, the obtained engineering bacteria have the advantages that the degradation efficiency is obviously improved, and the degradation rates of the NG-S1 (under anaerobic conditions) and the NG-S2 (under aerobic conditions) are increased by 2 times and 2.8 times respectively. The two high-temperature-resistant strains capable of efficiently degrading toxic pollutants respectively in an aerobic environment and in an anaerobic environment are provided, and have great significance and application value in treatment of environmental pollution; new methods and examples are provided for modification of thermophilic engineering bacteria for biodegradation.

The invention discloses a static fermentation high-temperature degrading bacterial agent for pigs died from diseases and application thereof, and the fermentation high-temperature degrading bacterialagent adopts strain W-3 or strain BA-3, the strain W-3 is classified as Geobacillus thermodenitrificans W-3, the accession number is CCTCC NO: M 2015458; the strain BA-3 is classified as Bacillus aestuarii BA-3, and the accession number is CCTCC NO: M 2015456. The fermentation high-temperature degrading bacterial agent is composed of the strain W-3 or / and strain BA-3, as well as a synergist and afly ash nano-modified filler. When in composting application, the fermentation high-temperature degrading bacterial agent can maintain high compost temperature and increase the activity of related enzymes and the rate of degradation. The fermentation high-temperature degrading bacterial agent can be used for the harmless treatment of the pigs died from diseases, helps to shorten the composting time, and completely removes pathogenic microorganisms in the pigs died from diseases, achieving an overall degradation rate of 95% or more, and an organic fertilizer with total nutrients of 5% or more can be finally formed.

The disclosure provides lantibiotics geobacillin I and geobacillin II from Geobacillus thermodenitrificans, compositions comprising the lantibiotics, and methods of use of the lantibiotics. Further disclosed are the sequences of lantibiotics geobacillin I and geobacillin I, and alternative structures of geobacillin I and geobacillin II comprising amino acid substitutions. Antimicrobial compositions comprising one or more isolated geobacillins and a pharmaceutically acceptable carrier, and methods of reducing reproduction of bacteria comprising administering to a subject a therapeutically effective amount of the antimicrobial composition are also provided.

The invention provides lantibiotics geobacillin I and geobacillin II from Geobacillus thermodenitrificans, compositions comprising the lantibiotics, and methods of use of the lantibiotics.

The invention belongs to the field of microorganisms, and discloses an artificially induced Geobacillus thermoglucosidase and its application in cellulose degradation. The applicant has isolated a cellulase enzyme from pig carcasses and samples of straw composting at high temperature. The most active strain Geobacillus thermodenitrificans , after high temperature acclimation and ultraviolet mutagenesis, a strain of Geobacillus thermoglucosidase with high cellulase production was obtained. Geobacillus glucosidase BGSC95A1. A variety of carbon sources can be used, and it can degrade a variety of celluloses, and the concentration of cellulase enzymes in the fermentation broth produced by fermentation is high.

The invention discloses a thermophilic bacterium compound strain obtained by mixing a Ureibacillus sp. strain, a thermophilic geobacillus sp. strain, a geobacillus thermodenitrificans strain and a rhodothermus marinus strain. The strain is obtained from the five strains through activation, culture, compounding and optimization. The compound strain has the advantages of short production cycle, large number of living bacteria and long preservation period, can resist the composting environment at a temperature higher than or equal to 60 DEG C and the like; inactivation of active microorganisms in the compound strain during ultrahigh-temperature aerobic composting can be avoided; the compound strain has higher adaptability and more stable performance than a single thermophilic inoculant, thereby having broad application prospect in rapid and harmless composting of organic waste.

A polynucleotide encoding a ThermoCas9 protein from Geobacillus thermodenitrificans and a constitutive promoter are used to engineer eukaryotic cells, e.g. fungi, yeast or algae, so that the ThermoCas9 endonuclease is integrated and expressed from the genome of the cell. Then, a second expression plasmid is used to transfect these ThermoCas9 expressing cells, the second plasmid containing an inducible promoter and a polynucleotide encoding a guide RNA. The guide RNA combines with the ThermoCas9 to provide the targeted endonuclease activity to cleave the cell DNA at a desired locus or gene of interest. A repair-oligo is also provided to the cell whereby following DNA cleavage, homologous recombination takes place in the cell with the repair-oligo so that either a deletion or substitution of nucleotides in the locus or gene of interest is achieved. Expression vectors and methods of using the vectors to achieve ThermoCas9 mediated gene editing are described whereby higher temperatures, e.g. greater than 30° C., are used.

The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a thermal denitrifying Geobacillus strain TB62 and its application in promoting compost maturity. A Geobacillus thermodenitrificans strain TB62, whose taxonomic name is Geobacillus thermodenitrificans TB62 (Geobacillus thermodenitrificans), was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 05, 2017, and the preservation number is for CGMCC 13530. The thermal denitrifying Geobacillus strain TB62 of the present invention is screened and isolated from samples in the high temperature period of composting for the first time. The agent can increase the compost temperature, accelerate the degradation of organic matter and water-soluble organic matter (DOM), increase the content of Kjeldahl nitrogen, and promote the maturity of compost; it also has the function of producing biosurfactant, so the strain can be used to prepare surfactant.

The invention discloses a dead pig static fermentation high-temperature degradation bacterial agent and its application. The high-temperature degradation bacterial agent for fermentation adopts bacterial strain W-3 or bacterial strain BA-3, and the classification of the bacterial strain W-3 is named Bacillus thermophilic denitrification W-3 (Geobacillus thermodenitrificans W-3), the preservation number is CCTCC NO: M 2015458; the taxonomic name of strain BA-3 is Bacillus BA-3 (Bacillus aestuarii BA-3), and the preservation number is CCTCC NO: M 2015456. The fermented high-temperature degradation bacteria agent is composed of strain W‑3 or / and strain BA‑3, synergist and fly ash nano-modified filler, which can maintain a high compost temperature and increase the activity of related enzymes during composting applications. increase the rate of degradation. The fermented high-temperature degradation bacteria agent can be used for the harmless treatment of sick and dead pigs, which helps to shorten the composting time, completely removes the pathogenic microorganisms in the sick and dead pigs, achieves an overall degradation rate of over 95%, and finally forms a total nutrient More than 5% organic fertilizer.

The present invention relates to a recombinant GRAS (Generally Recognized As Safe) strains expressing thermophilic arabinose isomerase as an active form and method of food grade tagatose by using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Geobacillus stearothermophilus DSM22 and Geobacillus thermodenitrificans, a recombinant expression vector containing the gene, a recombinant GRAS strains expressing the thermophilic arabinose isomerase as an active form by transformed with the expression vector, and a method of preparing food grade tagatose from galactose by using the same.

The invention relates to the technical field of microbial fermentation, and particularly discloses a sludge aerobic composting complex microbial inoculant as well as a preparation method and application thereof. The composite microbial agent is prepared from paenibacillus balunii TZ-1, bacillus licheniformis TD-4 and geobacillus thermodenitrificans HX-4. The invention further discloses a preparation method of the composite microbial agent. The sludge aerobic composting complex microbial inoculant provided by the invention has the advantages of high temperature rise speed, long high-temperature period maintenance time, high fermentation temperature, high decomposition speed, high organic matter degradation rate, high moisture content reduction speed and high seed germination index when being used for sludge composting, and can remarkably shorten the composting period and improve the composting quality.

The invention discloses a method for preparing emulsified heavy oil by using a microbial agent. The method comprises the following steps of: preparing any one of culture solutions A, B, C and D, sterilizing under high pressure, adding 25 to 50 percent of heavy oil and 1 to 5 percent of microbial emulsifier, and sealing; stirring at the temperature of between 50 and 75 DEG C and the rate of 50 to 150rpm in a vortex mode, and reacting for 6 to 24 hours; and standing for 2 to 4 hours after reaction is finished, and collecting an emulsified oil layer, namely the prepared emulsified heavy oil after oil and water are demixed, wherein the microbial agent is geobacillus thermodenitrificans. The process is simple, low in cost and environment-friendly, the required equipment is simple, series emulsified heavy oil products with different water content (20 to 60 percent) can be prepared according to requirements, the prepared emulsified heavy oil is stable in performance, the oil and the water are difficult to demix, a novel biotechnology for preparing the emulsified heavy oil is provided, and a good application prospect is achieved.

The invention relates to geobacillus thermodenitrificans HX-4 as well as a method for producing cellulase and application thereof. The preservation number of the geobacillus thermodenitrificans HX-4 is CGMCC (China General Microbiological Culture Collection Center) NO.24155; the geobacillus thermodenitrificans HX-4 can be used for preparing cellulase, the strain is inoculated into an LB culture medium to be cultured to prepare a seed solution, and the seed solution growing to the logarithmic phase is inoculated into a fermentation culture medium according to the inoculation volume of 3%-5% to be cultured to obtain fermentation liquor containing cellulase. The geobacillus thermodenitrificans HX-4 can normally grow under the condition of 55-70 DEG C, the activity of cellulase generated by the geobacillus thermodenitrificans HX-4 can reach 33.52 U / mL, after the geobacillus thermodenitrificans HX-4 is applied to sludge-rice hull powder mixed compost, the cellulose content of the geobacillus thermodenitrificans HX-4 is reduced by 26.81%, and the geobacillus thermodenitrificans HX-4 has great application value in the field of resource treatment and utilization of solid waste such as sludge and livestock and poultry manure.

Feed specially used for male goats in a goat mating stage is provided. The feed includes sorghum straw, cichorium intybus, silphium perfoliatum L., safflower seed, enteromorpha polysaccharides, medium-chain triglycerides, dietary fiber, a plant salt, Chinese buckeye fruit, ginsenoside, riboflavin, arginine, xylanase, lecithin, an anti-blocking agent, tartaric acid, and beta-cryptoxanthin. The content of sodium chloride in the plants salt is 20.5-30.2%, and the plant salt is formed by mixing a suaeda glauca plant salt and a samphire plant salt in a mass ratio of 1:2.5. A preparing method includes fine-powder fermentation utilizing first fermentation composite bacteria. The first fermentation composite bacteria include streptococcus thermophilus, lactobacillus, methanothermobacter thermoautotrophicus, bacillus subtilis and geobacillus thermodenitrificans. The feed greatly reduces the rate of cough symptoms of male goats in the mating stage and the rate of cough symptoms is reduced to 0.9-1.3%.