30 results about "Geobacillus thermodenitrificans" patented technology

The invention particularly relates to a compound fermentation agent in an ectopic fermentation treatment system of livestock and poultry manure. The compound microbial agent for treating manure by ectopic fermentation is composed of the following strains: Geobacillus toebii, Geobacillus stearothermophilus, Aeribacillus pallidus, Psychrobacter faecalis and Geobacillus thermodenitrificans. The compound agent is applied to an ectopic fermentation bed and has the advantages of rapid low-temperature start property, high fermentation temperature, high manure decomposition speed, large water volatilization amount and the like, and defects of low manure decomposition efficiency and high padding consumption rate of an existing microbial agent are overcome.

The disclosure provides lantibiotics geobacillin I and geobacillin II from Geobacillus thermodenitrificans, compositions comprising the lantibiotics, and methods of use of the lantibiotics. Further disclosed are the sequences of lantibiotics geobacillin I and geobacillin I, and alternative structures of geobacillin I and geobacillin II comprising amino acid substitutions. Antimicrobial compositions comprising one or more isolated geobacillins and a pharmaceutically acceptable carrier, and methods of reducing reproduction of bacteria comprising administering to a subject a therapeutically effective amount of the antimicrobial composition are also provided.

The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a mixed bacterial agent and its application in promoting compost maturity. A kind of mixed bacterial agent, the seed liquid of Bacillus urea strain TB42, the seed liquid of Bacillus licheniformis strain TA65 and the seed liquid of Bacillus thermodenitrative strain TB62 are respectively inoculated to 1L inoculation amount by 1% (v / v). In the LB culture solution after bacteria, cultivate in a shaker (50°C, 120rmp) for 24h, collect the culture solution, and obtain the mixed bacterial agent. The mixed microbial agent of the invention can increase the compost temperature, accelerate the degradation of organic matter and DOM, increase the content of Kjeldahl nitrogen, and promote the compost maturity.

The invention discloses a thermophilic bacterium compound strain obtained by mixing a Ureibacillus sp. strain, a thermophilic geobacillus sp. strain, a geobacillus thermodenitrificans strain and a rhodothermus marinus strain. The strain is obtained from the five strains through activation, culture, compounding and optimization. The compound strain has the advantages of short production cycle, large number of living bacteria and long preservation period, can resist the composting environment at a temperature higher than or equal to 60 DEG C and the like; inactivation of active microorganisms in the compound strain during ultrahigh-temperature aerobic composting can be avoided; the compound strain has higher adaptability and more stable performance than a single thermophilic inoculant, thereby having broad application prospect in rapid and harmless composting of organic waste.

The present invention relates to a recombinant GRAS (Generally Recognized As Safe) strains expressing thermophilic arabinose isomerase as an active form and method of food grade tagatose by using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Geobacillus stearothermophilus DSM22 and Geobacillus thermodenitrificans, a recombinant expression vector containing the gene, a recombinant GRAS strains expressing the thermophilic arabinose isomerase as an active form by transformed with the expression vector, and a method of preparing food grade tagatose from galactose by using the same.

The invention discloses a method for preparing emulsified heavy oil by using a microbial agent. The method comprises the following steps of: preparing any one of culture solutions A, B, C and D, sterilizing under high pressure, adding 25 to 50 percent of heavy oil and 1 to 5 percent of microbial emulsifier, and sealing; stirring at the temperature of between 50 and 75 DEG C and the rate of 50 to 150rpm in a vortex mode, and reacting for 6 to 24 hours; and standing for 2 to 4 hours after reaction is finished, and collecting an emulsified oil layer, namely the prepared emulsified heavy oil after oil and water are demixed, wherein the microbial agent is geobacillus thermodenitrificans. The process is simple, low in cost and environment-friendly, the required equipment is simple, series emulsified heavy oil products with different water content (20 to 60 percent) can be prepared according to requirements, the prepared emulsified heavy oil is stable in performance, the oil and the water are difficult to demix, a novel biotechnology for preparing the emulsified heavy oil is provided, and a good application prospect is achieved.