30 results about "Geobacillus thermodenitrificans" patented technology

The invention particularly relates to a compound fermentation agent in an ectopic fermentation treatment system of livestock and poultry manure. The compound microbial agent for treating manure by ectopic fermentation is composed of the following strains: Geobacillus toebii, Geobacillus stearothermophilus, Aeribacillus pallidus, Psychrobacter faecalis and Geobacillus thermodenitrificans. The compound agent is applied to an ectopic fermentation bed and has the advantages of rapid low-temperature start property, high fermentation temperature, high manure decomposition speed, large water volatilization amount and the like, and defects of low manure decomposition efficiency and high padding consumption rate of an existing microbial agent are overcome.

The disclosure provides lantibiotics geobacillin I and geobacillin II from Geobacillus thermodenitrificans, compositions comprising the lantibiotics, and methods of use of the lantibiotics. Further disclosed are the sequences of lantibiotics geobacillin I and geobacillin I, and alternative structures of geobacillin I and geobacillin II comprising amino acid substitutions. Antimicrobial compositions comprising one or more isolated geobacillins and a pharmaceutically acceptable carrier, and methods of reducing reproduction of bacteria comprising administering to a subject a therapeutically effective amount of the antimicrobial composition are also provided.

The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a mixed bacterial agent and its application in promoting compost maturity. A kind of mixed bacterial agent, the seed liquid of Bacillus urea strain TB42, the seed liquid of Bacillus licheniformis strain TA65 and the seed liquid of Bacillus thermodenitrative strain TB62 are respectively inoculated to 1L inoculation amount by 1% (v / v). In the LB culture solution after bacteria, cultivate in a shaker (50°C, 120rmp) for 24h, collect the culture solution, and obtain the mixed bacterial agent. The mixed microbial agent of the invention can increase the compost temperature, accelerate the degradation of organic matter and DOM, increase the content of Kjeldahl nitrogen, and promote the compost maturity.

The invention discloses a thermophilic bacterium compound strain obtained by mixing a Ureibacillus sp. strain, a thermophilic geobacillus sp. strain, a geobacillus thermodenitrificans strain and a rhodothermus marinus strain. The strain is obtained from the five strains through activation, culture, compounding and optimization. The compound strain has the advantages of short production cycle, large number of living bacteria and long preservation period, can resist the composting environment at a temperature higher than or equal to 60 DEG C and the like; inactivation of active microorganisms in the compound strain during ultrahigh-temperature aerobic composting can be avoided; the compound strain has higher adaptability and more stable performance than a single thermophilic inoculant, thereby having broad application prospect in rapid and harmless composting of organic waste.

A polynucleotide encoding a ThermoCas9 protein from Geobacillus thermodenitrificans and a constitutive promoter are used to engineer eukaryotic cells, e.g. fungi, yeast or algae, so that the ThermoCas9 endonuclease is integrated and expressed from the genome of the cell. Then, a second expression plasmid is used to transfect these ThermoCas9 expressing cells, the second plasmid containing an inducible promoter and a polynucleotide encoding a guide RNA. The guide RNA combines with the ThermoCas9 to provide the targeted endonuclease activity to cleave the cell DNA at a desired locus or gene of interest. A repair-oligo is also provided to the cell whereby following DNA cleavage, homologous recombination takes place in the cell with the repair-oligo so that either a deletion or substitution of nucleotides in the locus or gene of interest is achieved. Expression vectors and methods of using the vectors to achieve ThermoCas9 mediated gene editing are described whereby higher temperatures, e.g. greater than 30° C., are used.

The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a thermal denitrifying Geobacillus strain TB62 and its application in promoting compost maturity. A Geobacillus thermodenitrificans strain TB62, whose taxonomic name is Geobacillus thermodenitrificans TB62 (Geobacillus thermodenitrificans), was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 05, 2017, and the preservation number is for CGMCC 13530. The thermal denitrifying Geobacillus strain TB62 of the present invention is screened and isolated from samples in the high temperature period of composting for the first time. The agent can increase the compost temperature, accelerate the degradation of organic matter and water-soluble organic matter (DOM), increase the content of Kjeldahl nitrogen, and promote the maturity of compost; it also has the function of producing biosurfactant, so the strain can be used to prepare surfactant.

The invention discloses a dead pig static fermentation high-temperature degradation bacterial agent and its application. The high-temperature degradation bacterial agent for fermentation adopts bacterial strain W-3 or bacterial strain BA-3, and the classification of the bacterial strain W-3 is named Bacillus thermophilic denitrification W-3 (Geobacillus thermodenitrificans W-3), the preservation number is CCTCC NO: M 2015458; the taxonomic name of strain BA-3 is Bacillus BA-3 (Bacillus aestuarii BA-3), and the preservation number is CCTCC NO: M 2015456. The fermented high-temperature degradation bacteria agent is composed of strain W‑3 or / and strain BA‑3, synergist and fly ash nano-modified filler, which can maintain a high compost temperature and increase the activity of related enzymes during composting applications. increase the rate of degradation. The fermented high-temperature degradation bacteria agent can be used for the harmless treatment of sick and dead pigs, which helps to shorten the composting time, completely removes the pathogenic microorganisms in the sick and dead pigs, achieves an overall degradation rate of over 95%, and finally forms a total nutrient More than 5% organic fertilizer.

The present invention relates to a recombinant GRAS (Generally Recognized As Safe) strains expressing thermophilic arabinose isomerase as an active form and method of food grade tagatose by using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophilic Geobacillus stearothermophilus DSM22 and Geobacillus thermodenitrificans, a recombinant expression vector containing the gene, a recombinant GRAS strains expressing the thermophilic arabinose isomerase as an active form by transformed with the expression vector, and a method of preparing food grade tagatose from galactose by using the same.

The invention discloses a method for preparing emulsified heavy oil by using a microbial agent. The method comprises the following steps of: preparing any one of culture solutions A, B, C and D, sterilizing under high pressure, adding 25 to 50 percent of heavy oil and 1 to 5 percent of microbial emulsifier, and sealing; stirring at the temperature of between 50 and 75 DEG C and the rate of 50 to 150rpm in a vortex mode, and reacting for 6 to 24 hours; and standing for 2 to 4 hours after reaction is finished, and collecting an emulsified oil layer, namely the prepared emulsified heavy oil after oil and water are demixed, wherein the microbial agent is geobacillus thermodenitrificans. The process is simple, low in cost and environment-friendly, the required equipment is simple, series emulsified heavy oil products with different water content (20 to 60 percent) can be prepared according to requirements, the prepared emulsified heavy oil is stable in performance, the oil and the water are difficult to demix, a novel biotechnology for preparing the emulsified heavy oil is provided, and a good application prospect is achieved.