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A kind of mixed bacteria agent and its application in promoting compost maturity

A technology of mixing bacterial agents and strains, which is applied in application, preparation of organic fertilizers, bacteria, etc., can solve the problems of reduced utilization rate, achieve the effects of increasing content, increasing compost temperature, and promoting compost maturity

Active Publication Date: 2020-07-28
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year

Method used

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  • A kind of mixed bacteria agent and its application in promoting compost maturity
  • A kind of mixed bacteria agent and its application in promoting compost maturity
  • A kind of mixed bacteria agent and its application in promoting compost maturity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 : Screening of three strains of Bacillus urea strain TB42, Bacillus licheniformis strain TA65 and Geobacillus thermodenitrative strain TB62

[0033] 1. Experimental materials and equipment

[0034] 1.1 Main reagents

[0035] Table 1 Main Reagents

[0036] Drug Name molecular formula Specification ABTS C 18 h 24 N 6 o 6 S 4

AR hydrogen peroxide h 2 o 2

AR citric acid C 6 h 8 o 7

AR Sodium citrate Na 3 C 6 h 5 o 7

AR glacial acetic acid CH 3 COOH

AR Anhydrous sodium acetate CH 3 COONa

AR xylose C 5 h 10 o 5

AR Xylan (C 5 h 8 o 4 ) n

AR sodium tartrate C 4 h 5 Na 2 o 6

AR tartaric acid C 4 h 6 o 6

AR Veratrol C 8 h 12 o 3

AR lactic acid C 3 h 6 o 3

AR sodium lactate C 3 h 5 o 3 Na

AR Manganese sulfate MnSO 4

AR s...

Embodiment 2

[0132] Example 2 : identification of three strains

[0133] 2.1 The main instruments of the experiment

[0134] Table 4 The main instruments of the experiment

[0135]

[0136] 2.2 Experimental steps

[0137] 2.2.1 DNA extraction

[0138] DNA was extracted using Sangon's "Ezup Column Bacterial Genomic DNA Extraction Kit Instructions". Please refer to the instruction manual for specific operation steps.

[0139] 2.2.2 PCR amplification

[0140] Use 2×Es Taq MasterMix for PCR amplification, and the reaction system is as follows:

[0141]

[0142]

[0143] Reaction procedure:

[0144]

[0145] 2.2.3 PCR product electrophoresis results

[0146] Use 1% concentration of agarose gel electrophoresis, voltage 120V, electrophoresis 30min, load 1uL per hole, see the electrophoresis pattern Figure 9 .

[0147] Such as Figure 9 As shown, the results of PCR product electrophoresis indicated that the bacterial 16S rDNA fragment was successfully amplified.

[0148] ...

Embodiment 3

[0155] Example 3 : Application of mixed bacterial agent in promoting compost maturity

[0156] 3.1 Bacteria preparation

[0157] Inoculate the TB42, TB62 and TA65 seed liquids into 1L of sterilized LB culture solution at an inoculum amount of 1% (v / v), culture them in a shaker (50°C, 120rmp) for 24 hours, and collect the culture solution. For bacteria. The bacteria-adding group was the experimental group (T), and the blank group (CK) was added with 1 L of sterilized LB culture solution.

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Abstract

The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a mixed bacterial agent and its application in promoting compost maturity. A kind of mixed bacterial agent, the seed liquid of Bacillus urea strain TB42, the seed liquid of Bacillus licheniformis strain TA65 and the seed liquid of Bacillus thermodenitrative strain TB62 are respectively inoculated to 1L inoculation amount by 1% (v / v). In the LB culture solution after bacteria, cultivate in a shaker (50°C, 120rmp) for 24h, collect the culture solution, and obtain the mixed bacterial agent. The mixed microbial agent of the invention can increase the compost temperature, accelerate the degradation of organic matter and DOM, increase the content of Kjeldahl nitrogen, and promote the compost maturity.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a mixed bacterial agent and its application in promoting compost maturity. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production. However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year. Residues of pesticides and chemical fertilizers have released a large amount of harmful substances to the environment, polluting soil, water sources and food, and posing a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food has become a top priority. Microbial ferti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/20C12R1/07C12R1/10
CPCC05F3/00C05F17/00C12N1/20C12N1/205C12R2001/07C12R2001/10C05F11/00C05F11/08Y02W30/40
Inventor 李群良陆彦宇徐佳琦
Owner GUANGXI UNIV
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