Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mixed inoculant and application thereof in promotion of compost decomposition

A technology of mixing bacterial agents and strains, which is applied in the fields of application, preparation of organic fertilizers, bacteria, etc., can solve the problems of reduced utilization rate, achieve the effects of increasing content, accelerating DOM degradation, and increasing composting temperature

Active Publication Date: 2017-07-25
GUANGXI UNIV
View PDF7 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of mixture helps improve soil quality for better use on agricultural crops or other purposes like forestry production. It also increases its effectiveness at reducing harmful chemicals released from manure waste treatment processes that are used during landfill disposal operations.

Problems solved by technology

This patent describes different types of microbiological fertils called Rhizobium, which help plants grow better without causing negative side impacts on their surrounding environments such as soil, aquatic waters, air, land, and crops. These fertiles contain live organisms that work together like roots to provide benefits over time. They act synergistically when combined with certain beneficial compounds produced during industrial processes. Overall, these technical problem addressed by the patented technology involves finding ways to create safer and sustainable farming systems while reducing costs associated therewith.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mixed inoculant and application thereof in promotion of compost decomposition
  • Mixed inoculant and application thereof in promotion of compost decomposition
  • Mixed inoculant and application thereof in promotion of compost decomposition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 : Screening of three strains of urea bacillus strain TB42, bacillus licheniformis strain TA65 and thermal denitrifying geobacillus strain TB62

[0033] 1. Experimental materials and equipment

[0034] 1.1 Main reagents

[0035] Table 1 Main Reagents

[0036] Drug Name molecular formula Specification ABTS C 18 h 24 N 6 o 6 S 4

AR hydrogen peroxide h 2 o 2

AR citric acid C 6 h 8 o 7

AR Sodium citrate Na 3 C 6 h 5 o 7

AR glacial acetic acid CH 3 COOH

AR Anhydrous sodium acetate CH 3 COONa

AR xylose C 5 h 10 o 5

AR Xylan (C 5 h 8 o 4 ) n

AR sodium tartrate C 4 h 5 Na 2 o 6

AR tartaric acid C 4 h 6 o 6

AR Veratrol C 8 h 12 o 3

AR lactic acid C 3 h 6 o 3

AR sodium lactate C 3 h 5 o 3 Na

AR Manganese sulfate MnSO 4

AR ...

Embodiment 2

[0132] Example 2 : identification of three strains

[0133] 2.1 The main instruments of the experiment

[0134] Table 4 The main instruments of the experiment

[0135]

[0136] 2.2 Experimental steps

[0137] 2.2.1 DNA extraction

[0138] DNA was extracted using Sangon's "Ezup Column Bacterial Genomic DNA Extraction Kit Instructions". Please refer to the instruction manual for specific operation steps.

[0139] 2.2.2 PCR amplification

[0140] Use 2×Es Taq MasterMix for PCR amplification, and the reaction system is as follows:

[0141]

[0142]

[0143] Reaction procedure:

[0144]

[0145] 2.2.3 PCR product electrophoresis results

[0146] Use 1% concentration of agarose gel electrophoresis, voltage 120V, electrophoresis 30min, load 1uL per well, see the electrophoresis pattern Figure 9 .

[0147] like Figure 9 As shown, the results of PCR product electrophoresis indicated that the bacterial 16S rDNA fragment was successfully amplified.

[0148] 2.2...

Embodiment 3

[0155] Example 3 : Application of mixed bacterial agent in promoting compost maturity

[0156] 3.1 Preparation of bacterial agent

[0157] Inoculate the TB42, TB62 and TA65 seed liquids into 1L of sterilized LB culture solution at an inoculum amount of 1% (v / v), culture them in a shaker (50°C, 120rmp) for 24 hours, and collect the culture solution. For bacteria. The group added with bacterium was the experimental group (T), and the blank group (CK) was added with 1L of sterilized LB culture solution.

[0158] 3.2 Composting experiment

[0159] Cow dung and sugarcane leaves are selected as compost raw materials, with a mass ratio of 17:3, totaling 20kg. Composting was carried out for 45 days, samples were taken at 0, 5, 10, 16, 23, 30, and 45 days respectively, and parameters such as temperature, pH, moisture content, organic matter, Kjeldahl nitrogen, inorganic nitrogen, and DOM content were measured. The whole composting process was inoculated with bacteria twice, respe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of the development of plant growth promoting bacteria, and concretely relates to a mixed inoculant and an application thereof in the promotion of compost decomposition. The mixed inoculant is obtained through the following steps: inoculating 1L of a sterilized LB nutrient solution with a Ureibacillus suwonensis strain TB42 seed solution, a Bacillus licheniformis strain TA65 seed liquid and a Geobacillus thermodenitrificans strain TB62 seed solution at a respective inoculation amount of 1% (v/v), culturing the above strains in a shaking bed (50 DEG C, 120 rpm) for 24 hours, and collecting the obtained culture solution. The mixed inoculant improves the compost temperature, accelerates the degradation of organic matters and the degradation of DOMs, improves the content of Kjeldahl nitrogen and promotes the decomposition of compost.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com