A kind of thermophilic bacteria compound bacterial agent and its preparation method and application
A technology of thermophilic bacteria and compound bacteria, applied in the direction of biochemical equipment and methods, applications, and preparation of organic fertilizers, can solve the problems of shortening the composting treatment time, etc., and achieve long storage period, short production period and strong adaptability Effect
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Embodiment 1
[0029] The composting effect comparison of embodiment 1 single bacterial strain and mixed bacterial strain
[0030] In this example, the 5 species of bacteria are: Bacillus thermourea A, Geobacillus B, Bacillus thermodenitrophilus C, Halothermophilus rubrum D and Thermus thermophilus E, including the following steps :
[0031] (1) Strain activation: Inoculate 5 strains of thermophilic bacteria stored at low temperature on solid medium, culture them at 60°C for 24 hours, pick a single colony and connect them to the slant medium, cultivate them at 60°C for 24 hours, and use an equal amount of The bacteria on the surface of the culture medium were washed with water as the inoculum, and the viable bacteria in each inoculum were greater than 1×10 8 CFU / g;
[0032] (2) Compounding of mixed strains: the inoculum of 5 strains of thermophilic strains obtained in step (1) was processed according to Table 1 to obtain the inoculum of thermophilic bacteria / combined inoculum;
[0033] Ta...
Embodiment 2
[0045] The determination of embodiment 2 optimum strain proportioning
[0046] In this example, the 5 species of bacteria are: Bacillus thermourea A, Geobacillus B, Bacillus thermodenitrophilus C, Halothermophilus rubrum D and Thermus thermophilus E, including the following steps :
[0047] (1) Strain activation: Inoculate 5 strains of thermophilic bacteria stored at low temperature on solid medium, culture them at 60°C for 24 hours, pick a single colony and connect them to the slant medium, cultivate them at 60°C for 24 hours, and use an equal amount of The bacteria on the surface of the culture medium were washed with water as the inoculum; the amount of viable bacteria in each inoculum was greater than 1×10 8 CFU / g
[0048] (2) Compounding of mixed strains: the inoculum of 5 strains of thermophilic strains obtained in step (1) was mixed according to the ratio in Table 3 to obtain the compound inoculum of thermophilic bacteria;
[0049] Table 3 The inoculum volume ratio o...
Embodiment 3
[0061] The compost effect comparison of embodiment 3 different compost raw materials composition
[0062] In this example, the 5 species of bacteria are: Bacillus thermourea A, Geobacillus B, Bacillus thermodenitrophilus C, Halothermophilus rubrum D and Thermus thermophilus E, including the following steps :
[0063] Operation steps (1)-(4) are the same as in Example 2, except that the addition ratio of 5 strains of bacterial agents is 1:1:1:1:1, and the compound inoculum of thermophilic bacteria is prepared; compound inoculation The amount of viable bacteria in the liquid is greater than 1×10 8 CFU / g;
[0064] (5) In terms of mass percentage, mix according to the composting material proportioning in table 5, obtain original material, adjust the water content of original material after being 55%, insert the liquid bacterial agent that step (4) obtains, its addition ratio is 0.025% (accounting for the mass percentage of the original material) to obtain the fermentation raw m...
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