Static fermentation high-temperature degrading bacterial agent for pigs died from diseases and application thereof
A technology for high-temperature degradation and disease-dead pigs. It is applied in the fields of application, bacteria, and climate change adaptation. It can solve the problems of incomplete removal of pathogenic microorganisms, slow degradation of dead pigs, and short high-temperature maintenance time, so as to shorten composting time and increase activity. , the effect of increasing the degradation rate
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Embodiment 1
[0021] A fermenting high-temperature degrading bacterium, which is bacterial strain W-3, and the classification of said bacterial strain W-3 is named as Geobacillus thermodenitrificans W-3 (Geobacillus thermodenitrificans W-3), which has been preserved on July 17, 2015 in China Center for Type Culture Collection, the deposit number is CCTCC NO: M 2015458, and the deposit address is Wuhan University, Wuhan, China.
[0022] The cultivation process is as follows: select beef extract peptone medium (made of beef extract 3g, peptone 10g, sodium chloride 5g, water 1000ml, pH value is 7.2) for cultivation; culture conditions are: temperature is 58°C, pH value is 7.2 , the oscillation frequency is 250r / min, and the ventilation volume is 3.5L / min.
[0023] Colony morphology: opaque raised radial colonies with leafy edges and off-white dull surface.
[0024] Bacteria morphology: blunt round rods at both ends of the bacterium, spores in the middle, cysts without obvious expansion, and t...
Embodiment 2
[0026] A fermenting high-temperature degrading bacterium, which is strain BA-3, and the classification of said strain BA-3 is named Bacillus aestuarii BA-3, which has been preserved in Chinese Type Culture on July 17, 2015 The depository center, the deposit number is CCTCC NO: M 2015456.
[0027] The cultivation process is as follows: select beef extract peptone medium (made of beef extract 3g, peptone 10g, sodium chloride 5g, water 1000ml, pH value is 7.2) for cultivation; culture conditions are: temperature is 58°C, pH value is 7.2 , the oscillation frequency is 250r / min, and the ventilation volume is 3.5L / min.
[0028] Colony morphology: approximately round colonies, white colonies with soft texture and slightly glossy surface.
[0029] Bacterial morphology: the thallus is rod-shaped, with blunt round ends, mesozoic and sometimes apical, with round ends, and the size of the thallus is 3.0-3.8μm×1.0μm.
Embodiment 3
[0031] A fermentation high-temperature degradation bacterial agent includes the above-mentioned cultivated bacterial strain W-3, and also includes synergists and fly ash nano-modified fillers. Among them, fly ash nano-modified filler is mainly made of fly ash and coal slime, and nano-AL 2 o 3 As a nano-modified filler, the coal slime is selected from coal slime with ash content between 50-65% and moisture content between 12-20%; wherein the synergist consists of 40wt% soybean meal, 30wt% methylcellulose, 15wt% Composed of refined sugar and 15% by weight table salt.
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