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Thermophilic bacterium compound strain as well as preparation method and application thereof

A technology of thermophilic bacteria and compound bacteria, which is applied in the direction of biochemical equipment and methods, application, and preparation of organic fertilizers, can solve the problems of shortening the composting treatment time, and achieve long storage period, stable performance, and effective number of viable bacteria high effect

Active Publication Date: 2017-09-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for a single thermophilic strain to adapt to the aerobic composting of various organic wastes. Therefore, the development of a compound microbial inoculant compounded by a variety of thermophilic strains for the ultra-high temperature composting process of organic waste can greatly improve the inoculation of the compound microbial inoculant. Adapt to the scope, promote the degradation rate of various organic substances, shorten the composting treatment time, and have potential application value in the field of organic waste composting treatment

Method used

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  • Thermophilic bacterium compound strain as well as preparation method and application thereof
  • Thermophilic bacterium compound strain as well as preparation method and application thereof
  • Thermophilic bacterium compound strain as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The composting effect comparison of embodiment 1 single bacterial strain and mixed bacterial strain

[0030] In this example, the 5 species of bacteria are: Bacillus thermourea A, Geobacillus B, Bacillus thermodenitrophilus C, Halothermophilus rubrum D and Thermus thermophilus E, including the following steps :

[0031] (1) Strain activation: Inoculate 5 strains of thermophilic bacteria stored at low temperature on solid medium, culture them at 60°C for 24 hours, pick a single colony and connect them to the slant medium, cultivate them at 60°C for 24 hours, and use an equal amount of The bacteria on the surface of the culture medium were washed with water as the inoculum, and the viable bacteria in each inoculum were greater than 1×10 8 CFU / g;

[0032] (2) Compounding of mixed strains: the inoculum of 5 strains of thermophilic strains obtained in step (1) was processed according to Table 1 to obtain the inoculum of thermophilic bacteria / combined inoculum;

[0033] Ta...

Embodiment 2

[0045] The determination of embodiment 2 optimum strain proportioning

[0046] In this example, the 5 species of bacteria are: Bacillus thermourea A, Geobacillus B, Bacillus thermodenitrophilus C, Halothermophilus rubrum D and Thermus thermophilus E, including the following steps :

[0047] (1) Strain activation: Inoculate 5 strains of thermophilic bacteria stored at low temperature on solid medium, culture them at 60°C for 24 hours, pick a single colony and connect them to the slant medium, cultivate them at 60°C for 24 hours, and use an equal amount of The bacteria on the surface of the culture medium were washed with water as the inoculum; the amount of viable bacteria in each inoculum was greater than 1×10 8 CFU / g

[0048] (2) Compounding of mixed strains: the inoculum of 5 strains of thermophilic strains obtained in step (1) was mixed according to the ratio in Table 3 to obtain the compound inoculum of thermophilic bacteria;

[0049] Table 3 The inoculum volume ratio o...

Embodiment 3

[0061] The compost effect comparison of embodiment 3 different compost raw materials composition

[0062] In this example, the 5 species of bacteria are: Bacillus thermourea A, Geobacillus B, Bacillus thermodenitrophilus C, Halothermophilus rubrum D and Thermus thermophilus E, including the following steps :

[0063] Operation steps (1)-(4) are the same as in Example 2, except that the addition ratio of 5 strains of bacterial agents is 1:1:1:1:1, and the compound inoculum of thermophilic bacteria is prepared; compound inoculation The amount of viable bacteria in the liquid is greater than 1×10 8 CFU / g;

[0064] (5) In terms of mass percentage, mix according to the composting material proportioning in table 5, obtain original material, adjust the water content of original material after being 55%, insert the liquid bacterial agent that step (4) obtains, its addition ratio is 0.025% (accounting for the mass percentage of the original material) to obtain the fermentation raw m...

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Abstract

The invention discloses a thermophilic bacterium compound strain obtained by mixing a Ureibacillus sp. strain, a thermophilic geobacillus sp. strain, a geobacillus thermodenitrificans strain and a rhodothermus marinus strain. The strain is obtained from the five strains through activation, culture, compounding and optimization. The compound strain has the advantages of short production cycle, large number of living bacteria and long preservation period, can resist the composting environment at a temperature higher than or equal to 60 DEG C and the like; inactivation of active microorganisms in the compound strain during ultrahigh-temperature aerobic composting can be avoided; the compound strain has higher adaptability and more stable performance than a single thermophilic inoculant, thereby having broad application prospect in rapid and harmless composting of organic waste.

Description

technical field [0001] The invention relates to the field of microbial inoculum, in particular to a thermophilic compound inoculum applied to ultra-high temperature aerobic composting and its preparation method and application. Background technique [0002] Composting is currently the most commonly used effective method for harmless and resourceful treatment of organic waste. Organic waste (such as livestock and poultry manure, sick and dead livestock and poultry, straw, kitchen waste, etc.) usually contains rich N, P and a certain amount of trace elements. After aerobic composting, the pathogenic bacteria and parasites in it are eliminated. It is the most suitable fertilizer for crop growth. However, under the condition of natural aerobic composting, affected by factors such as the type and quantity of indigenous microorganisms in the compost, the temperature rises slowly in the initial stage of composting. , the duration of the high temperature period is short, and there ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/01
CPCC05F3/00C05F17/00C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 钱玉婷杜静黄红英曹云王琳靳红梅徐跃定
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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