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Sludge aerobic composting complex microbial inoculant as well as preparation method and application thereof

A technology of aerobic composting and compound bacterial agents, applied in the direction of sewage/sludge fertilizer, biochemical equipment and methods, applications, etc., can solve the problems of slow degradation rate of organic matter, short maintenance time of high temperature period, long time to reach high temperature period, etc. problems, achieve the effects of reducing the content of toxic substances, high seed germination index, and improving quality

Active Publication Date: 2022-04-15
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, anaerobic digestion is the most widely used, but there are potential safety hazards and high requirements for operation and management; composting is a biological treatment method, because it can make full use of the components in sludge, turn waste into treasure, and is conducive to the establishment of a recycling system. Economical, but most of the bacterial agents currently used for sludge composting have the defects of slow decomposing speed, long time to reach high temperature period, short high temperature period maintenance time, slow organic matter degradation rate and poor decomposing effect, and it is difficult to achieve a better decomposing degree

Method used

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  • Sludge aerobic composting complex microbial inoculant as well as preparation method and application thereof
  • Sludge aerobic composting complex microbial inoculant as well as preparation method and application thereof
  • Sludge aerobic composting complex microbial inoculant as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0034] A kind of sludge aerobic composting compound bacteria agent, including Paenibacillus barensis ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitrifying ( Geobacillus thermodenitrificans ) HX-4;

[0035] The Paenibacillus barensis ( Paenibacillus barengoltzii ) The deposit number of TZ-1 is CGMCCNo.23696, which was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on November 1, 2021, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Bacillus licheniformis ( Bacillus licheniformis ) TD-4, whose deposit number is CGMCC NO.24154, was deposited on December 21, 2021 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Thermodenitrifying Geobacillus ( Geobacillus the...

Embodiment 2

[0047] A kind of sludge aerobic composting compound bacterial agent, comprises the Paenibacillus barensis in embodiment 1 ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitrifying ( Geobacillus thermodenitrificans ) HX-4;

[0048] The preparation method of above-mentioned sludge aerobic composting compound bacterial agent is:

[0049] 1) Preparation of medium: LB liquid medium: 8g sodium chloride, 8g peptone, 4g yeast extract powder, make up to 1000mL with distilled water;

[0050] Lipase fermentation medium: glucose 4g, peptone 1.8g, urea 5g, dipotassium hydrogen phosphate trihydrate 0.8g, ammonium sulfate 0.8g, magnesium sulfate heptahydrate 0.4g, tributyrin 8mL, distilled water to make up to 1000mL;

[0051] Protease fermentation medium: glucose 1.8g, peptone 1.8g, calcium carbonate 1.8g, ferrous sulfate 0.4g, ammonium sulfate 0.8g, magnesium sulfate heptahydrate 1.8g, dipotassium hydrogen phosphate 1...

Embodiment 3

[0059] A kind of sludge aerobic composting compound bacterial agent, comprises the Paenibacillus barensis in embodiment 1 ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitrifying ( Geobacillus thermodenitrificans ) HX-4;

[0060] The preparation method of above-mentioned sludge aerobic composting compound bacterial agent is:

[0061] 1) Preparation of medium: LB liquid medium: 12g sodium chloride, 12g peptone, 6g yeast extract powder, make up to 1000mL with distilled water;

[0062] Lipase fermentation medium: glucose 6g, peptone 2.2g, urea 7g, dipotassium hydrogen phosphate trihydrate 1.2g, ammonium sulfate 1.2g, magnesium sulfate heptahydrate 0.6g, tributyrin 12mL, distilled water to make up to 1000mL;

[0063] Protease fermentation medium: glucose 2.2g, peptone 2.2g, calcium carbonate 2.2g, ferrous sulfate 0.6g, ammonium sulfate 1.2g, magnesium sulfate heptahydrate 2.2g, dipotassium hydrogen phosphat...

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Abstract

The invention relates to the technical field of microbial fermentation, and particularly discloses a sludge aerobic composting complex microbial inoculant as well as a preparation method and application thereof. The composite microbial agent is prepared from paenibacillus balunii TZ-1, bacillus licheniformis TD-4 and geobacillus thermodenitrificans HX-4. The invention further discloses a preparation method of the composite microbial agent. The sludge aerobic composting complex microbial inoculant provided by the invention has the advantages of high temperature rise speed, long high-temperature period maintenance time, high fermentation temperature, high decomposition speed, high organic matter degradation rate, high moisture content reduction speed and high seed germination index when being used for sludge composting, and can remarkably shorten the composting period and improve the composting quality.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a sludge aerobic composting composite bacterial agent and its preparation method and application. Background technique [0002] With the continuous improvement of the level of urbanization, the amount of urban sewage treatment is also increasing, accompanied by a large amount of sludge. Sludge has the characteristics of large capacity, instability, perishability, and foul smell. If it is not treated effectively, it will cause serious secondary environmental pollution to its stacking and discharge areas, and even cause irreversible poisoning of the soil, affecting The growth of surrounding crops and the pollution of crops. [0003] Traditional sludge treatment methods include incineration, landfill and resource utilization. The current treatment methods mostly use incineration and landfill methods, but the incineration method has huge investment and is easy to cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05F7/00C12R1/10C12R1/01
CPCY02A40/20
Inventor 韩永辉滕志楠崔建升李清扬黄亚丽秦学安鸿雪李再兴周淋友王艳魁刘馨岳杨添琪吕建伟刘峰
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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