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A dead pig static fermentation high temperature degradation bacterial agent and its application

A technology for high-temperature degradation and disease-dead pigs. It is applied in the fields of application, bacteria, and climate change adaptation. It can solve the problems of slow degradation of dead pigs, short high-temperature maintenance time, and incomplete removal of pathogenic microorganisms, so as to shorten composting time and increase activity. , the effect of increasing the degradation rate

Active Publication Date: 2021-01-01
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a static fermentation high-temperature degradation bacterial agent for dead pigs and its application. The degradative bacterial agent realizes high-temperature rapid degradation during composting, and is applied to the harmless treatment of sick pigs, solving the problem of high-temperature maintenance time in traditional composting methods Short, the degradation rate of dead pigs is slow, and the pathogenic microorganisms in them cannot be completely removed

Method used

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  • A dead pig static fermentation high temperature degradation bacterial agent and its application
  • A dead pig static fermentation high temperature degradation bacterial agent and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A fermenting high-temperature degrading bacterium, which is bacterial strain W-3, and the classification of said bacterial strain W-3 is named as Geobacillus thermodenitrificans W-3 (Geobacillus thermodenitrificans W-3), which has been preserved on July 17, 2015 in China Center for Type Culture Collection, the deposit number is CCTCC NO: M 2015458, and the deposit address is Wuhan University, Wuhan, China.

[0022] The cultivation process is as follows: select beef extract peptone medium (made of beef extract 3g, peptone 10g, sodium chloride 5g, water 1000ml, pH value is 7.2) for cultivation; culture conditions are: temperature is 58°C, pH value is 7.2 , the oscillation frequency is 250r / min, and the ventilation volume is 3.5L / min.

[0023] Colony morphology: opaque raised radial colonies with leafy edges and off-white dull surface.

[0024] Bacteria morphology: blunt round rods at both ends of the bacterium, spores in the middle, cysts without obvious expansion, and t...

Embodiment 2

[0026] A fermenting high-temperature degrading bacterium, which is strain BA-3, and the classification of said strain BA-3 is named Bacillus aestuarii BA-3, which has been preserved in Chinese Type Culture on July 17, 2015 The depository center, the deposit number is CCTCC NO: M 2015456.

[0027] The cultivation process is as follows: select beef extract peptone medium (made of beef extract 3g, peptone 10g, sodium chloride 5g, water 1000ml, pH value is 7.2) for cultivation; culture conditions are: temperature is 58°C, pH value is 7.2 , the oscillation frequency is 250r / min, and the ventilation volume is 3.5L / min.

[0028] Colony morphology: approximately round colonies, white colonies with soft texture and slightly glossy surface.

[0029] Bacterial morphology: the thallus is rod-shaped, with blunt round ends, mesozoic and sometimes apical, with round ends, and the size of the thallus is 3.0-3.8μm×1.0μm.

Embodiment 3

[0031] A fermentation high-temperature degradation bacterial agent includes the above-mentioned cultivated bacterial strain W-3, and also includes synergists and fly ash nano-modified fillers. Among them, fly ash nano-modified filler is mainly made of fly ash and coal slime, and nano-AL 2 o 3 As a nano-modified filler, the coal slime is selected from coal slime with ash content between 50-65% and moisture content between 12-20%; wherein the synergist consists of 40wt% soybean meal, 30wt% methylcellulose, 15wt% Composed of refined sugar and 15% by weight table salt.

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Abstract

The invention discloses a dead pig static fermentation high-temperature degradation bacterial agent and its application. The high-temperature degradation bacterial agent for fermentation adopts bacterial strain W-3 or bacterial strain BA-3, and the classification of the bacterial strain W-3 is named Bacillus thermophilic denitrification W-3 (Geobacillus thermodenitrificans W-3), the preservation number is CCTCC NO: M 2015458; the taxonomic name of strain BA-3 is Bacillus BA-3 (Bacillus aestuarii BA-3), and the preservation number is CCTCC NO: M 2015456. The fermented high-temperature degradation bacteria agent is composed of strain W‑3 or / and strain BA‑3, synergist and fly ash nano-modified filler, which can maintain a high compost temperature and increase the activity of related enzymes during composting applications. increase the rate of degradation. The fermented high-temperature degradation bacteria agent can be used for the harmless treatment of sick and dead pigs, which helps to shorten the composting time, completely removes the pathogenic microorganisms in the sick and dead pigs, achieves an overall degradation rate of over 95%, and finally forms a total nutrient More than 5% organic fertilizer.

Description

technical field [0001] The invention relates to the technical field of harmless treatment of sick and dead pigs, in particular to a static fermentation high-temperature degradation bacterial agent for sick and dead pigs and an application thereof. Background technique [0002] In recent years, a large number of sick and dead pigs have appeared in some areas of China, and the food safety problems caused by the continuous illegal entry of sick and dead pigs into the food chain have attracted great attention from the State Council and other relevant departments. Sick and dead pigs that have not been properly disposed of will seriously affect my country's ecological environment, agricultural production, and food safety. Improper handling of sick and dead pig carcasses will cause serious social harm: 1) endanger the safety of the ecological environment. If the corpses of sick and dead pigs with unknown causes are thrown indiscriminately and buried, it will inevitably pollute the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F1/00C05F17/20C12R1/01C12R1/07
CPCC05F1/00C05F17/00C12N1/20C05F17/20C12N1/205C12R2001/01C12R2001/07C05F11/08Y02W30/40Y02A40/20
Inventor 苏世广徐雅芫王银昌李海龙钱坤
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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