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Geobacillus thermodenitrificans DC8 strain and application thereof

A geobacillus, denitrification technology, applied in bacteria, organic chemistry, chemical instruments and methods, etc., can solve the problems of difficult separation and screening, limited hydrolysis capacity of excess sludge, low protein extraction rate, etc., and has broad application prospects. , Improve the acquisition of nitrogen sources, obvious economic benefits

Pending Publication Date: 2022-03-11
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some scholars try to screen and domesticate heat-resistant microorganisms to apply to the biochemical treatment system of refractory excess sludge, but because high temperature is conducive to the lysis of excess sludge, for the strains that degrade excess sludge at high temperature, it is difficult to isolate them in practice. There are still some difficulties in screening and screening. Even if some heat-resistant microorganisms are isolated, their ability to hydrolyze excess sludge is limited, and the extraction rate of protein is low.

Method used

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  • Geobacillus thermodenitrificans DC8 strain and application thereof
  • Geobacillus thermodenitrificans DC8 strain and application thereof
  • Geobacillus thermodenitrificans DC8 strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Isolation and screening of thermodenitrifying Geobacillus DC8

[0034] The separation and screening method of thermal denitrifying Geobacillus DC8 of the present invention is obtained by adopting a gradient dilution separation method, and the method comprises the following steps:

[0035] (a) Collecting samples: High-temperature composting substrates were collected from a kitchen waste treatment and disposal center in Shijiazhuang.

[0036] (b) Sample pretreatment: take the above sample into a sterilized container filled with sterile water, seal it and shake it evenly for 30 minutes, let the suspension stand, take the supernatant and centrifuge, and take the upper bacterial liquid as the bacterial suspension ,spare.

[0037] (c) Enrichment culture: Take 2 mL of the bacterial suspension and inoculate it into 200 mL of liquid medium, and place it in a constant temperature incubator at 60°C for 48 hours to obtain a culture medium for expanding strains.

[0038]...

Embodiment 2

[0044] Example 2 Identification of Physical and Chemical Properties of Thermal Denitrifying Geobacillus DC8

[0045] The identification method of thermal denitrifying Geobacillus DC8 of the present invention comprises the following steps:

[0046] (1) Observe the colony shape and strain shape of the thermophilic strain DC8. The thermophilic bacteria of the present invention were cultured at 60°C for 12 hours. figure 1 shown.

[0047] The thermophilic bacterial strain DC8 of the present invention belongs to the thermophilic Bacillus genus and is Gram-positive. figure 2 shown.

[0048] (2) Physiological and biochemical characteristics of the strain

[0049] .

[0050] (3) DNA sequence information and phylogenetic analysis

[0051] MEGA7.0 software was used to display the 16S rDNA sequence phylogenetic tree of the thermophilic strain DC8 and related strains by the adjacent connection method, and the similarity was repeated 1000 times. image 3 The middle growth tree node ...

Embodiment 3

[0053] Example 3 Lysis test of thermal denitrifying Geobacillus DC8

[0054] using plateau growing E. coli as the model strain for the experiment. E. coli The culture solution was collected by centrifugation at 5000rpm for 10min and resuspended. 50% (V / V) of thermophilic bacteria (Abs 600 =1.0~1.5) The crude enzyme liquid obtained after centrifugation of Geobacillus thermodenitrifying DC8 was added to the lysis system. Finally, 100mL of mixed bacterial solution was added to a 250mL Erlenmeyer flask and treated at 60°C for 16h, the results were as follows: Figure 4 shown.

[0055] Depend on Figure 4 It can be seen that with the increase of time, E. coli The cell lysis rate continued to increase and reached a maximum of 63.6% at 14 hours. It shows that the hydrolase secreted by Geobacillus thermodenitrifying DC8 will E. coli The cells are lysed, the intracellular organic matter is released, and the content of soluble organic matter in the supernatant increases.

...

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Abstract

The invention relates to a geobacillus thermodenitrificans DC8 strain and application thereof, the geobacillus thermodenitrificans DC8 strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2021, and the preservation number is CGMCC No.23318. The strain provided by the invention has the capability of degrading excess sludge to extract protein in a high-temperature environment, and the protein extraction rate can reach 29.7%. Protein in sludge can be effectively utilized by utilizing the strain, the reduction and degradation effect of residual sludge is improved, and the strain has a good application prospect. According to the invention, an efficient bacterium source is provided for the reduction of the residual sludge of a sewage plant, the functional application of the geobacillus thermodenitrificans is widened, and the geobacillus thermodenitrificans has a relatively high practical value.

Description

technical field [0001] The invention belongs to the technical field of sludge treatment, and in particular relates to a thermodenitrifying Geobacillus DC8 strain and an application thereof. Background technique [0002] Sludge, as waste generated in the process of sewage treatment, has complex components and carries a large number of microorganisms, pathogenic bacteria, heavy metals, and organic pollutants. The main disposal methods include landfill, disposal, incineration, and recycling. Improper treatment is likely to cause secondary pollution, which will not only affect the treatment capacity of the sewage treatment system, but also pose a serious threat to the ecological environment and human activities. [0003] The remaining sludge is rich in organic matter, and the protein content in the intracellular and extracellular polymers is 30% to 60% of the dry weight of the sludge. In order to avoid resource waste, recycling protein in excess sludge is an important excess sl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C07K1/14C12R1/01
CPCC02F11/02C07K1/145
Inventor 钟为章丁雅婷朱琳刘曼宁志芳秦学李再兴
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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