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Biotechnology capable of producing No. 380 admiralty fuel oil and application thereof

A technology for fuel oil and ships, which is applied in the direction of microorganisms, microorganisms, and methods based on microorganisms. It can solve the problems that the quality indicators are difficult to meet international standards, it is difficult to meet the needs of enterprises and markets, and the output is limited. It is easy to reproduce and popularize. , the effect of increasing value

Active Publication Date: 2013-07-03
南京贝尔多蒙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No matter which method, the cost is high, the operation is more complicated, the output is limited, the quality index is difficult to meet the international standard, and it is difficult to meet the needs of enterprises and the market.

Method used

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  • Biotechnology capable of producing No. 380 admiralty fuel oil and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of microbial agent: the thermophilic denitrophilic Bacillus NG80-2 ( Geobacillus thermodenitrificans , the strain is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and its preservation number is CGMCC No.1228) and Mycobacterium archaea ( Mycobacterium goodii sp., the strain was purchased from the American Culture Collection, and its preservation number is ATCC No. 700504) were inoculated into the culture medium sterilized by autoclaving at a ratio of 1:1000 (v / v), and vortexed at 50°C and 150rpm , airtight (oxygen) culture for 18 hours, and the obtained bacterial solution was mixed at a ratio of 1:1 by volume. The culture medium is: peptone 1.6%; yeast extract 1%; sodium chloride 0.5%; water balance.

Embodiment 2

[0024] Activation and expanded cultivation of microbial agents: Inoculate the mixed microbial agents at a ratio of 1:1000 (v / v) into the culture medium sterilized by autoclaving, vortex and stir at 50°C, 150rpm, and incubate in airtight (oxygenation) for 18 hours , to obtain activated microbial bacterial agents. The composition of the culture medium is: peptone 1.6%; yeast extract 1%; sodium chloride 0.5%; water balance.

Embodiment 3

[0026] Method 1 for the production of No. 380 ship fuel oil using microbial agents:

[0027] (1) Mix the activated 1% microbial agent, 50% oil slurry and the rest of the inorganic salt solution by weight percentage, vortex and stir at 40°C and greater than 50rpm, and seal the reaction for 24 hours;

[0028] (2) After the reaction, let it stand for more than 2 hours, and collect the oil layer with a separating funnel after the oil and water are separated. This oil product is the No. 380 marine fuel oil produced.

[0029] The test results show that the kinematic viscosity of the treated oil is 312, and other indicators also meet the quality standards of No. 380 marine fuel oil (Table 1).

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Abstract

The invention discloses a method of adopting biotechnology to produce admiralty fuel oil, taking oil slurry as raw materials. According to the method, microbial agent composed of activated thermophilic denitriding bacillus NG80-2 (Geobacillus thermodenitrificans) and Mycobacterium goodiisp at the ratio of 1:1 is reacted with high-kinematic viscosity oil slurry in an inorganic salt solution to obtain the No. 380 admiralty fuel oil. A reaction system comprises 1-5%t of activated mixed agents, 50% of oil slurry and the balance of inorganic salt solution. The production method has the advantages of simple process, simplicity in equipment, low cost and environment protection, can produce fuel oil with good quality, and has better processing effect for the oil slurry; and the produced oil product can completely meet the standard of the No. 380 admiralty fuel oil, and has better application prospect.

Description

technical field [0001] The invention belongs to the technical field of fuel oil production, and relates to a production method of marine fuel oil, more specifically, a method and application for producing No. 380 marine fuel oil by using oil slurry as a raw material and adopting biotechnology. Background technique [0002] Fuel oil is a kind of refined oil, which is the heavy residual product separated from crude oil after gasoline, kerosene and diesel oil in the process of petroleum processing. It is widely used in ship boilers, heating furnaces, metallurgical furnaces and other industrial fuels. According to the use, fuel oil can be divided into marine fuel oil, furnace fuel oil and other fuel oil. No. 380 marine fuel oil is an important marine fuel oil. The quality standards of this type of fuel oil are: kinematic viscosity at 50°C is less than 380CST, flash point is greater than 60°C, pour point is less than 30°C, sulfur content is less than 3.5%, and ash content is less...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C10G31/00C12R1/01
Inventor 王威张宝亮
Owner 南京贝尔多蒙生物科技有限公司
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