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Biological synthesis method of dehydroepiandrosterone

A technology of dehydroepiandrosterone and its synthesis method, which is applied in the field of biological preparation of dehydroepiandrosterone, and can solve the problems of easy transformation into androstenedione, low yield, poor product quality, etc.

Active Publication Date: 2021-10-12
湖南引航生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as: two equivalents of potassium tert-butoxide of explosive chemicals need to be used; the stability of simplified A in the middle is very poor, and it is easy to change into androstenedione and produce various oxidative impurities (Development of a Chemoenzymatic Process for Dehydroepiandrosterone Acetate Synthesis, Org.Process Res.Dev.2016,20,1520-1528) resulting in lower yield of reaction, poor quality of product

Method used

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  • Biological synthesis method of dehydroepiandrosterone
  • Biological synthesis method of dehydroepiandrosterone
  • Biological synthesis method of dehydroepiandrosterone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Determination of Androstenedione Acetate by High Performance Liquid Chromatography

[0037] Androstenedione Acetate Source: Prepared from Androstenedione

[0038] Reference: Chinese Journal of Medicinal Chemistry, Issue 5, Volume 23, 368-371

[0039] High performance liquid chromatography analysis method:

[0040]

[0041] The HPLC chromatogram obtained was figure 1 shown.

Embodiment 2

[0043] High Performance Liquid Chromatography Detection of Double Bond Shifted Intermediate A

[0044] The source of the double bond translocation intermediate A: prepared from androstenedione

[0045] Reference: Chinese patent: CN105695551B

[0046] High performance liquid chromatography analysis method:

[0047]

[0048] The HPLC chromatogram obtained was figure 2 shown.

Embodiment 3

[0050] High performance liquid chromatography detection of dehydroepiandrosterone (DHEA)

[0051] Source of dehydroepiandrosterone (DHEA): purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0052] High performance liquid chromatography analysis method:

[0053]

[0054] The HPLC chromatogram obtained was image 3 shown.

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Abstract

The invention discloses a biological preparation method of dehydroepiandrosterone. The preparation method starts from steroid androstenedione ester which is low in price and easy to obtain to finally obtain a target product with the yield up to 94% through a one-step biological enzyme catalytic reaction. In the process, an intermediate A is not accumulated, so that oxidizing impurities caused by instability of the intermediate A cannot be generated. Therefore, high reaction yield and good product quality can be achieved.

Description

technical field [0001] The invention belongs to the technical fields of biopharmaceutical and biochemical industry, and in particular relates to a biological preparation method of dehydroepiandrosterone. Background technique [0002] Dehydroepiandrosterone (DHEA) is a substance with important biological activity and various physiological functions. It plays an important role in maintaining normal physiological functions and prolonging life. The secretion of human DHEA peaks at the age of 25-30, and then declines year by year. A large number of clinical experiments have confirmed that a high level of DHEA in the human body is beneficial to human health. This compound has a wide range of applications, as a precursor of sex hormones, it has anti-aging and protein assimilation effects. The main functions are: 1. Improve sexual function and sexual desire; 2. Regulate immune system function and improve body immunity; 3. Enhance physical fitness, improve mood and sleep, and impr...

Claims

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Application Information

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IPC IPC(8): C12P41/00C12P33/06
CPCC12P41/002C12P33/06
Inventor 谢新开黄晓飞梁晓亮
Owner 湖南引航生物科技有限公司
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