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Content determination method used for sinew and bone injury spray

A measurement method and a spray technology, which are applied in the field of pharmaceutical inventions, can solve the problems of ineffective detection of epimedium active ingredients, complicated operation, inability to effectively detect active ingredients of products, etc.

Active Publication Date: 2019-05-17
贵州远程制药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Muscle and Bone Injury Spray is a traditional Chinese medicine compound preparation made by extracting Chishan Powder, Radix Paeoniae Rubra, Epimedium, Earthworm, Aconitum Aconitum, and Menthol. It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and is used for soft tissue Injury, the active ingredients in the tendon and bone injury spray are: paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, baohuoside I, etc., but the tendon and bone injury spray There is no scientific detection method for these key active ingredients, so that it can achieve a more comprehensive quality detection
[0003] The quantitative index in the current quality standard of Jingushang Spray is paeoniflorin in Radix Paeoniae Rubra, which is not used much in prescriptions. For Chishan Powder, in the quality standard, it is only identified by TLC , this standard is too simple to achieve effective control of product quality
[0004] Existing literature Xu Chaofei, Quality research and quality standard improvement of Miao medicine "Jingushang Spray" [D], Guiyang Medical College, 2015, disclosed a simultaneous determination of 4 components 3,3' in Jingushang Spray by HPLC -Dimethylellagic acid, 3,3',4'-trimethylellagic acid, paeoniflorin, and icariin. This method simultaneously determines the content of four components in a traditional Chinese medicine preparation, but There are many components that affect the quality of tendon and bone injuries. Only measuring 4 of them cannot effectively detect the active ingredients in the product, and methylellagic acid accounts for 2 of them. Experiments have shown that there are many active ingredients in Epimedium, and only measuring Obviously, icariin cannot effectively detect the active ingredients in Epimedium, so this method cannot scientifically determine the content of active ingredients in the tendon and bone injury spray. Determination of reference substance, complicated operation and low efficiency

Method used

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  • Content determination method used for sinew and bone injury spray
  • Content determination method used for sinew and bone injury spray
  • Content determination method used for sinew and bone injury spray

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The content of the active ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, and baohuoside I in Jingushang Spray was detected according to the following method.

[0033] Chromatographic conditions: Agilent ZORBA*S8~C18 column; the inner diameter is 4.6*150mm, 5um, the mobile phase B is 0.05mol / L potassium dihydrogen phosphate solution, the mobile phase C is methanol, the mobile phase D is acetonitrile, and the detection wavelength is 0 ~23min: 230nm, 23~54min: 254nm, 54~137min: 270nm, column temperature 35℃, gradient elution method:

[0034] time

mobile phase B

mobile phase C

mobile phaseD

flow rate

0~14min

92％～90％

——

8％～10％

1ml / min

14~17.5min

90％

——

10％

1ml / min

17.5~22min

90％～88％

——

10％～12％

1ml / min

22~33min

88％～83％

——

12％～17％

1ml / min

33~50min

83％～79％

——

17％～21％

1ml / min

50~51min

79％～77.5％

——

21％～2...

Embodiment 2

[0042] The content of the active ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, and baohuoside I in Jingushang Spray was detected according to the following method.

[0043] Chromatographic conditions: Agilent ZORBA*S8~C18 column; the inner diameter is 4.6*150mm, 5um, the mobile phase B is 0.05mol / L potassium dihydrogen phosphate solution, the mobile phase C is methanol, the mobile phase D is acetonitrile, and the detection wavelength is 0 ~23min: 230nm, 23~54min: 254nm, 54~137min: 270nm, column temperature 35℃, gradient elution method:

[0044]

[0045]

[0046] Preparation of the test solution: Take 10ml of tendon and bone injury spray, accurately absorb, put it in a 100ml evaporating dish, evaporate to dryness, add 5ml of aqueous solution to dissolve, transfer the solution to a 25ml test tube, add 10ml of water-saturated n-butanol solution in the test tube , extract, add 10ml of water-saturated n-butanol solution for extraction, t...

Embodiment 3

[0051] The content of the active ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, and baohuoside I in Jingushang Spray was detected according to the following method.

[0052] Chromatographic conditions: Agilent ZORBA*S8~C18 column; the inner diameter is 4.6*150mm, 5um, the mobile phase B is 0.05mol / L potassium dihydrogen phosphate solution, the mobile phase C is methanol, the mobile phase D is acetonitrile, and the detection wavelength is 0 ~23min: 230nm, 23~54min: 254nm, 54~137min: 270nm, column temperature 35℃, gradient elution method is as follows:

[0053] time

mobile phase B

mobile phase C

mobile phaseD

flow rate

0~14min

92％～90％

——

8％～10％

1ml / min

14~17.5min

90％

——

10％

1ml / min

17.5~22min

90％～88％

——

10％～12％

1ml / min

22~33min

88％～83％

——

12％～17％

1ml / min

33~50min

83％～79％

——

17％～21％

1ml / min

50~51min

79％～77.5％ ...

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Abstract

The invention relates to a content determination method used for sinew and bone injury spray, and specifically relates to a content determination method for seven effective constituents, such as paeoniflorin, icariin, ellagic acid, Bepimedin A, Bepimedin B, Bepimedin C and Baohuoside I, in the sinew and bone injury spray. The method comprises preparation of test solution, preparation of referencesolution and HPLC determination. With the content determination method provided by the invention, various effective constituents can be determined simultaneously, a detection line is stable, degree ofseparation is high, stability is excellent, detection accuracy is high, content determination time is reduced, and detection efficiency is improved; in order to control quality of the sinew and boneinjury spray, a relatively comprehensive quantitative detection method is provided, meanwhile, for inspecting stability of the sinew and bone injury spray, a method of stability quantitative inspection for relatively comprehensive effective constituents is provided.

Description

technical field [0001] The invention relates to the field of medical inventions, in particular to a method for determining the content of a spray for tendons and bones. Background technique [0002] Muscle and Bone Injury Spray is a traditional Chinese medicine compound preparation made by extracting Chishan Powder, Radix Paeoniae Rubra, Epimedium, Earthworm, Aconitum Aconitum, and Menthol. It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and is used for soft tissue Injury, the active ingredients in the tendon and bone injury spray are: paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, baohuoside I, etc., but the tendon and bone injury spray There is no scientific detection method for these key active ingredients, so that it can achieve a more comprehensive quality detection. [0003] The quantitative index in the current quality standard of Jingushang Spray is paeoniflorin in Radix Paeoni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 伍庆程吉祥刘青唐勇薛静罗天军伍朝平王娇李佳蔚
Owner 贵州远程制药有限责任公司
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