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Detecting method of traditional Chinese medicine composition for treating rheumatoid arthritis

A rheumatoid and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of consuming manpower, material resources, financial resources, consuming a lot of materials, and difficult to extract and purify active ingredients. The method is simple and convenient, ensuring clinical The effect of curative effect

Active Publication Date: 2019-01-11
GUANGZHOU BAIYUNSHAN CHENLIJI PHARMA FAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In this method, the standard curve method is adopted to establish the standard curves of hyperin and quercitrin respectively. This detection method is accurate, reliable, simple and fast, and has laid a foundation for improving the quality control level of Houttuynia cordata. Quality control is not easy. It is necessary to determine the standard curve of various flavonoids, which consumes manpower, material resources and financial resources. Moreover, some active ingredients are difficult to extract and purify, and it is difficult to obtain reference substances whose purity meets the established standard curve. , not recommended as a quality control for traditional Chinese medicine compositions
[0006] It is well known in the art that external standard method or internal standard method is mostly used in the prior art to determine the content of multiple index components in traditional Chinese medicine compositions, but external standard method or internal standard method needs to provide reference substances and internal standards for various index components. In order to achieve the industrialization goal of green environmental protection and saving, it is necessary to provide a simple method for treating rheumatoid arthritis traditional Chinese medicine composition that can detect multiple active ingredients at the same time.

Method used

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  • Detecting method of traditional Chinese medicine composition for treating rheumatoid arthritis
  • Detecting method of traditional Chinese medicine composition for treating rheumatoid arthritis
  • Detecting method of traditional Chinese medicine composition for treating rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the detection of Chinese medicine composition for the treatment of rheumatoid arthritis

[0031] The one-test-multiple-evaluation method of the HPLC detection method is adopted, and the steps are as follows:

[0032] Preparation of S1 mixed reference solution: Accurately weigh hyperoside, epimedin B, epimedin A, epimedin C, icariin, luteolin, quercetin, nobiletin, kaempferol and Baohuoside Ⅰ reference substance, dissolved in 80% methanol solution to a constant volume, shaken well, obtained containing 96.8 μg / ml hyperin, 119.04 μg / ml epimedin B, 213.2 μg / ml epimedin A, 257.32 μg / ml ml epimedin C, 390 μg / ml icariin, 4.136 μg / ml luteolin, 42.24 μg / ml quercetin, 16.69 μg / ml nobiletin, 24.416 μg / ml kaempferol and 17.984 μg / ml po Mixed reference substance solution of Huoside I;

[0033] S2 chromatographic conditions: detector Agilent diode array detector, model 1200; chromatographic column is octadecylsilane bonded silica gel reversed-phase chromatographic col...

Embodiment 2

[0040] Embodiment 2, the sample treatment of Chinese medicine composition for the treatment of rheumatoid arthritis

[0041] Preparation of the sample to be tested: accurately weigh the powder of the traditional Chinese medicine composition for treating rheumatoid arthritis, place it in a conical flask with a stopper, add 20 ml of absolute ethanol precisely, and mix the powder of the traditional Chinese medicine composition for treating rheumatoid arthritis with no Water-ethanol solid-liquid ratio 1g:66ml, weighed, ultrasonic 20min, cooled at room temperature, weighed, anhydrous ethanol supplemented the reduced weight, shake well, centrifuge at 10000r / min for 10min, take supernatant with 0.22μm micro The sample to be tested is obtained by filtering through a pore filter membrane.

[0042] Samples were injected and tested according to the method in Example 1, with an injection volume of 10 μl.

Embodiment 3

[0043] Example 3, sample processing of Chinese medicine composition for treating rheumatoid arthritis

[0044] Preparation of the sample to be tested: precisely weigh the powder of the traditional Chinese medicine composition for treating rheumatoid arthritis, place it in a conical flask with a stopper, add 20ml of 80% ethanol solution precisely, and mix the powder of the traditional Chinese medicine composition for the treatment of rheumatoid arthritis with 80% ethanol solution with a solid-liquid ratio of 1g:68ml, weighed, ultrasonicated for 40min, cooled at room temperature, weighed, 80% ethanol supplemented the reduced weight, shaken well, centrifuged at 10000r / min for 10min, and the supernatant was washed with 0.22 The sample to be tested is obtained by filtering through a μm microporous membrane.

[0045] Samples were injected and tested according to the method in Example 1, with an injection volume of 10 μl.

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Abstract

The invention belongs to the field of traditional Chinese medicine compositions, and particularly relates to a detecting method of a traditional Chinese medicine composition for treating rheumatoid arthritis. The traditional Chinese medicine composition for treating rheumatoid arthritis is composed of Tripterygium hypoglaucum, herba epimedii, semen cuscutae and fructus lycii. According to the detecting method of a traditional Chinese medicine composition for treating rheumatoid arthritis, 10 flavonoid compounds comprising hyperoside, caohuoside A, caohuoside C, caohuoside B, icariin, luteolin,quercetin, nobiletin, kaempferol and Baohuoside-I can be simultaneously detected by using a multi-evaluation method; and the hyperoside is taken as an internal referenc, so that the product quality of Tripterygium hypoglaucum, herba epimedii, semen cuscutae and fructus lycii is effectively controlled; the detecting method is simple and convenient, and lays a foundation for better ensuring the clinical curative effect of the traditional Chinese medicine composition for treating rheumatoid arthritis, and also provides a reference for the detection of traditional Chinese medicines rich in flavonoids.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine compositions, and in particular relates to a detection method of a traditional Chinese medicine composition for treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis is a chronic, inflammatory synovitis-based systemic disease of unknown etiology. It is characterized by polyarticular, symmetrical, and aggressive arthritis of the small joints of the hands and feet, often accompanied by extra-articular organ involvement and positive serum rheumatoid factor, which can lead to joint deformity and loss of function. Drugs for the treatment of rheumatoid arthritis Mainly non-steroidal anti-inflammatory drugs, glucocorticoids, biological agents and traditional Chinese medicine preparations. The treatment of traditional Chinese medicine and traditional Chinese medicine preparations shows the characteristics of overall regulation, multi-level and multi-target, and can a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张雪彭富全何风雷刘敏
Owner GUANGZHOU BAIYUNSHAN CHENLIJI PHARMA FAB CO LTD
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