43results about How to "Good stability and repeatability" patented technology

The invention discloses a preparing method for a tobacco-made extract. The invention includes the following steps: tobacco leaves are firstly blended with water and enzyme for enzymolysis; and then ethanol is applied for extraction, filtration and concentration; and lastly the tobacco-made extract is obtained through maillard reaction. The invention utilizes the tobacco dust generated from the tobacco production, takes into full consideration the feasibility and operability of industrial production with respect to the preparing process, and furthermore, conducts a series of exploration on factors relating to the enzymolysis, extraction and maillard reaction forms the final preparing process and optimal technical parameters, and thereby the product quality reaches the requirements for adding fragrance to the tobacco-made extract with outstanding steadiness and repeatability.

The invention relates to an electrochemical biosensor and application thereof, in particular to an electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof. The acetylcholinesterase is immobilized with chitosan by way of layer-by-layer self-assembly, but not immobilized by simple cross-linking. The biosensor detects organic phosphorus and carbamate pesticides at a higher sensitivity, and a prepared enzyme electrode is not easy to fall off has better repeatability, better stability and longer service life.

An enzyme-linked immunoassay method of bisphenol A belongs to the technical field of immunodetection. The enzyme-linked immunosorbent assay method utilizes the immunization of a synthetic bisphenol A immunogen to obtain a polyclonal antibody, takes the bisphenol A as a standard product, takes a conjugate of a hapten of diphenolic acid and OVA as a coating antigen and establishes the indirect competitive enzyme-linked immunosorbent assay method of the bisphenol A. The enzyme-linked immunosorbent assay method establishes the indirect competitive ELISA method of the bisphenol A and provides a rapid and high-efficient detection method for detecting the residual bisphenol A, as the method adopts the polyclonal antibody, the cost is lower, and the stability and the repeatability are good. The sensitivity is 0.1ng / ml, and the linear range is 0-100ng / ml. The high specificity and the affinity of the immune reaction lead the ELISA to have very high selectivity and sensitivity.

The invention relates to an enzyme-linked immunoassay method for dihexyl phthalate, and belongs to the technical field of immunodetection. The invention utilizes immunity of synthesized dihexyl phthalate immunogen to obtain a polyclonal antibody, and takes the dihexyl phthalate as standard and conjugate of dihexyl phthalate hapten and OVA as envelope antigen, to establish indirect competitive enzyme-linked immunoassay method for the dihexyl phthalate. The invention establishes the indirect competitive ELISA method for the dihexyl phthalate, and provides a quick and high-efficiency detecting method for detecting residual of the dihexyl phthalate. The method adopts the polyclonal antibody, so the cost is lower and the stability and repetitiveness are good; and high specificity and compatibility of an immunoreaction make the ELISA have extremely high selectivity and sensitivity, and the sensitivity is 0.01ng / mL and the linear range is between 0 and 100ng / mL.

The invention discloses a diagnostic reagent kit for trichinosis by employing dot-immunogold filtration assay. The invention has the following advantages: gold labeled SPA is taken as an antibody labeling tag, isopropyl beta-D-thiogalactoside is used for inducing the pMAL-c2X-Ts21 / TB1 recombinant strain, expressing and purifying trichina Ts21 gene-encoded protein (Ts21 antigenic gene, ORF17.20, the access number at GenBank is U88239), after being purified on a nitrocellulose membrane (NCM), the Ts21 recombinant protein coats the detection point T, and human IgG coats the quality control point C, thus constructing Ts21 recombinant protein-dot-immunogold filtration assay (Ts21-DIGFA) and assembling the rapid diagnostic reagent kit for trichina with Ts21 recombinant protein by employing dot-immunogold filtration assay. The kit is simple to operate and has high sensitivity and specificity and good stability and repeatability; the detection results can be observed and judged within 1min immediately after adding samples, the diagnosis results are accurate and clear and misjudgment can not happen; and the kit is suitable for rapid diagnosis and serum epidemiological survey at the scene and is easy to popularize and apply extensively.

The invention relates to an attenuation measurement device for a waveguide system. The attenuation measurement device comprises a radio-frequency signal output end, an attenuation signal input end, a radio-frequency signal source, a local oscillator signal source, a frequency divider, a frequency mixer, a semi-automatic inductive voltage divider and a phase-locked amplifier, the radio-frequency signal source generates a radio-frequency signal and a time-based signal, the radio-frequency signal is coupled to a radio-frequency signal input end, the local oscillator signal source takes the time-based radio-frequency signal as a reference signal and outputs a local oscillator signal, the radio-frequency signal and the local oscillator signal share a time base, the frequency divider is used for dividing the frequency for the time-based signal, the frequency mixer is used for mixing the frequency of the local oscillator signal and the frequency of the inputted attenuation signal, the semi-automatic inductive voltage divider is used for dividing the voltage of a frequency mixing signal outputted by the frequency mixer, and the phase-locked amplifier takes a frequency dividing signal outputted by the frequency divider as a reference signal and detects an output signal of the semi-automatic inductive voltage divider. The attenuation measurement device can measure a 50GHz-110GHz waveguide attenuation standard device, and has small signal receiving capability and fine stability and repeatability.

The invention relates to a method for extracting total triterpenoids and monomer ilicin A of enriched Hainan holly leaf. The method is characterized by comprising the following extraction steps that raw materials of Hainan holly leaf is subjected to reflux extraction by 50 percent to 95 percent of ethanol, liquid supernatant and a precipitation part are obtained after the extraction liquid is concentrated and centrifuged, the precipitation part is subjected to mixed suspension by 30 percent to 40 percent of ethanol, then, petroleum ether is used for extraction, the lower layer solution is subjected to pressure reduction, concentration and drying to obtain total triterpenoids; in addition, the ethanol extraction liquid is subjected to pressure reduction, recovery and evaporation to dryness, water is used for dissolving, acid is added for hydrolysis, organic solvents are extracted, the recovery to dryness, the silicagel column coating and the sherwood oil-ethyl acetate gradient elution are carried out, eluant is collected, elution solvents are recovered through pressure reduction, and the monomer ilicin A is obtained. According to an extraction enriching method, the total triterpenoids content of the obtained product exceeds 50 percent, the purity of the monomer ilicin A is high, meanwhile, the repeatability and the stability of the method are good, the transfer rate of effective ingredients of the raw material is high, the cost is low, the operation is simple and convenient, and the method is suitable for industrial production. Meanwhile, the invention also relates to application of the total triterpenoids and the monomer ilicin A of Hainan holly leaf in the cardiovascular disease treating aspect, in particular to the atherosclerosis aspect, and the potential development value is realized.

The invention discloses a method for detecting an HPLC specific chromatogram of an origin-preserving decoction. The method is characterized by comprising the following steps of (1) preparing a test solution, namely taking an origin-preserving decoction preparation to be detected, and preparing the test solution; (2) preparing a mixed reference substance solution, namely taking ginsenosides Rg1, Reand Rb1, and preparing the ginsenosides Rg1, Re and Rb1 into the mixed reference substance solution; and (3) formulating a specific chromatogram, namely respectively sucking the test solution and thereference substance solution, injecting the solutions into a high performance liquid chromatograph, and obtaining the specific chromatogram of the origin-preserving decoction according to a common peak in the chromatograms of more than N batches of tested products. The detection method provided by the invention can be used for effectively characterizing the variety and quantity of the chemical components in the origin-preserving decoction, and an effective detection method is provided for the comprehensive and effective quality control of the origin-preserving decoction.

The invention provides a method for preparing high-purity ethylhexylglycerin. According to the method, an intermediate 4-alkoxymethyl-1,3-dioxoane is generated from 2-ethylhexylglycidyl ether and acetone under the action of boron trifluoride diethyl etherate, a terminator is added in good time before hydrolysis, and after liquid separation, an oil-phase substance is neutralized by use of sodium hydrogen carbonate and then washed, next, a stabilizer is added, and finally, the high-purity ethylhexylglycerin is obtained by use of short-path distillation. The method is suitable for cosmetic additives and suitable for large-scale industrial production, and has the advantages of simple process, small energy consumption, product yield of greater than 88%, purity of 99.3%, no color and no taste, and the like.

The present invention discloses a method for detecting the vitamin D content in a vitamin D drop. According to the present invention, through a large number of optimizations, the optimal mobile phase composition, the optimal flow rate, the optimal detection wavelength, the optimal chromatography column and other analysis conditions are obtained, and the multiple experiment results show that the method has characteristics of good stability, good repeatability, high analysis efficiency and good separation, and can sensitively and accurately perform qualitative and quantitative detection on the vitamin D3, such that the quality of the vitamin D drop can be objectively, completely and accurately evaluated, and the important significance can be provided for the vitamin D drop quality control and the clinical treatment effect ensuring.

The invention discloses a fingerprint spectrum recognition method for Pu'er drying green raw tea and based on chemical components. The fingerprint spectrum recognition method comprises the following steps: establishing a standard fingerprint spectrum of the Pu'er drying green raw tea; establishing a fingerprint spectrum of tea to be measured in the same way; and comparing the fingerprint spectrum of the tea to be measured and the standard fingerprint spectrum of the Pu'er drying green raw tea, and screening the tea to be measured in accordance with the standard fingerprint spectrum of the Pu'er drying green raw tea. The pretreatment of the samples is simple and convenient, so a lot of manpower labor is not required to be spent. The recognition method for the Pu'er drying green raw tea and based on the whole chemical components has relatively good precision, stability and repeatability, and has high recognition rate.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a specific chromatogram construction method and a quality detection method of schizonepeta. Liquid chromatography conditions, mobile phase compositions and an elution procedure are controlled, and a specific chromatogram of nonvolatile constituents of the schizonepeta is established; suitable gas chromatography conditions are selected, and a specific chromatogram of volatile constituents of the schizonepeta is established; specific peaks of the specific chromatograms are marked; the liquidchromatography elution procedure is adjusted, and the content of hesperidin in the schizonepeta is determined; and at the same time, a quality control method of the schizonepeta is established, the quality control method comprises the steps that the schizonepeta specific chromatograms are used for evaluating the schizonepeta, the content of the hesperidin is determined, the quality control methodfurther comprises the steps that thin layer chromatography identification is conducted, heavy metal and hazardous elements are examined, the pesticide residue amount is determined, extraction determination is conducted, and the quality of the schizonepeta is evaluated from the perspective of effectiveness and safety. The quality detection method has the advantages of being simple, fast, stable, reliable, high in precision, good in reproducibility, easy to master, and the like.

The invention discloses a method for producing ZnSO alloy film with an adjustable sulfur-doped growth band gap, which uses a pulse laser deposition method. A target material is produced by mixing pure zinc oxide and pure zinc sulfide powder for hydraulic forming to obtain a ceramic target, wherein mole content of zinc sulfide is 10 to 40%; the target material is arranged in a growth chamber of a pulsed-laser deposition device, wherein the degree of vacuum is 1*10(-4) to 1*10(-3)Pa, the laser frequency is 3 to 10Hz, the growth temperature is from 300 to 500 DEG C, a ZnSO alloy film with an adjustable band gap is grown on a substrate. A real time doping can be realized according to the method provided in the invention, the doping concentration can be controlled by regulating the growth temperature and mole content of sulfur in the target material. Method for producing the ZnSO alloy film with adjustable sulfur-doped growth band gap provided in the invention has the advantages of good optical performance, repeatability and stability.

The invention discloses a fluorine-doped carbon coated lithium iron phosphate, and preparation method and application thereof. The method comprises the steps of firstly mixing and grinding prepared pure lithium iron phosphate with organic matters including F for several hours by using an organic solvent and then drying in a vacuum chamber, and calcinating at high temperatures for several hours in the atmosphere of inert gas and thus obtaining the fluorine-doped carbon coated lithium iron phosphate. The fluorine-doped carbon coated lithium iron phosphate prepared by using the method can be significantly improved in electrochemical performance, and can reach a specific discharge capacity of 150mAh / g.

The invention discloses a method for analyzing the contents of adenosine and cordycepin in Cordyceps martialis fruiting body and solid culture medium residue thereof by HPLC. The method comprises the following steps of: firstly preparing standard solutions of adenosine and cordycepin and sample solutions of Cordyceps martialis fruiting body and solid culture medium residue thereof; and then detecting the sample solutions of Cordyceps martialis fruiting body and solid culture medium residue thereof respectively under the conditions that the chromatographic column is XBridge TMC 18 (5 mum, 4.6*150 mm), the mobile phase is methanol and ultra-pure water at a volume ratio of 10:90, the flow rate is 1.0 ml / min, the sample size is 10 muL (automatic sample injection), the column temperature is 30 DEG C, and the detection wavelength is 254 nm, wherein the sample solutions should appear chromatographic peaks having the same chromatographic retention time as the cordycepin standard solution. The method provided by the invention is convenient and effective, is particularly suitable for analyzing the contents of adenosine and cordycepin in Cordyceps martialis fruiting body and solid culture medium residue thereof, and has good reproducibility and stability.

The present invention relates to a detection method for a dose of ionizing radiation on human peripheral blood lymphocytes. The detection method mainly comprises: designing primers and a Taqman-MGB probe according to a lymphocyte gdf15 gene expression sequence, and constructing a recombinant vector containing the lymphocyte gdf15 gene expression sequence as a standard substance; adopting the 10-fold serial diluted standard substance to carry out real-time fluorescence PCR, and drawing an absolute quantification standard curve; carrying out quantitative determination on the expression levels of the gdf15 gene of lymphocytes with different culture times after ionizing radiations with different doses to obtain a dose-effect fitting curve of the radiation dose and the gdf15 gene expression level; and carrying out quantitative determination on the expression level of the gdf15 gene of lymphocytes with the radiation dose requiring detection, and calculating the dose of the ionizing radiation on the lymphocytes requiring detection. According to the present invention, the dose of ionizing radiation on human peripheral blood lymphocytes can be rapidly and quantitatively detected so as to meet requirements of simpleness, rapid quantitation and high throughput, and the advantages can be provided when the large-scale radiation accident occurs.

The invention relates to the technical field of Sanhuang granule detection, in particular to a Sanhuang granule detection method, which comprises the following specific steps: S1, taking Sanhuang granules respectively, and performing thin-layer chromatography identification test on radix astragali preparata, prepared rhubarb and turmeric in the Sanhuang granules; S2, respectively detecting the durability of radix astragali preparata, prepared rhubarb and turmeric in the Sanhuang granules according to a thin-layer method under different temperatures, different humidity, thin-layer plates of different manufacturers, different sample application amounts and different developing solvent developing effects; and S3, determining the contents of aloe-emodin, rheinic acid, emodin, chrysophanol and physcion in the prepared rhubarb. According to the invention, the content and medicinal value of the radix astragali preparata, the prepared rhubarb and the turmeric in the Sanhuang granules can be effectively analyzed by adopting a thin-layer chromatography identification test on the radix astragali preparata, and the content and medicinal value of the radix astragali preparata, the prepared rhubarb and the turmeric in the Sanhuang granules can be effectively analyzed, so that the scheme is high in precision, good in repeatability and stability, simple and convenient to operate and high in detection efficiency so as to ensure the quality and the medicinal value of the Sanhuang granules.

The invention discloses a method for development of specific PCR markers of SNP loci related to an F-type three-line hybrid wheat male sterility gene, wherein the method comprises the steps: (1) carrying out multi-generation sister crossing on wheat, dividing the obtained selfing lines into a maintainer line with high self-fruitful rate, a maintainer line with medium self-fertility rate, and a stable sterile line with self-fruitful rate low than 5%, wherein the three lines are identical in other biological characteristics except having fertility difference; (2) carrying out library-building and sequencing of the three samples; (3) searching for SNP variations between mitochondrial and cell nucleus genomes of the three samples; and (4) aiming at mitochondrial and cell nucleus genome SNP loci obtained after identification of the sterile line and the maintainer lines with different fruitfulness rates, detecting, designing and developing restriction endonuclease amplified polymorphism sequence (CAPS) markers or competitive allele specific PCR (KASP) markers. The invention also discloses relevant SNP loci molecular markers and a detection method thereof.

The invention relates to a quality control method for a rhizoma polygonatum medicinal material. The method comprises the following steps of a construction method of an HPLC fingerprint spectrum of therhizoma polygonatum medicinal material, a thin layer chromatography analysis method, a water content and total ash content measurement method and an extractum and total saponin measurement method; the construction method of the HPLC fingerprint spectrum of the rhizoma polygonatum medicinal material comprises the step of preparation of a test solution, wherein fine rhizoma polygonatum powder is collected, water is added for ultrasonic treatment, centrifugation is conducted, and a supernate is collected; after evaporation to dryness is conducted, hydrochloric acid is added for dissolution, andwater bath treatment is conducted; methyl alcohol is dropwise added to take residual hydrochloric acid away; residues after being evaporated to dryness are dissolved with methyl alcohol, centrifugation and filtration through a microfiltration membrane are conducted, and the test solution is obtained; according to the construction method of the HPLC fingerprint spectrum of the rhizoma polygonatum medicinal material, the same peak value exists for different rhizoma polygonatum medicinal materials, the precision, repeatability and stability are good, the similarity, to a contrast spectrum, of thefingerprint spectrum is 0.99 or greater, and the HPLC fingerprint spectrum is good in separation degree and peak shape, the quality of three kinds of rhizoma polygonatum can be comprehensively reflected, and counterfeit medicinal products are identified based on the reason.

The invention provides a polarization insensitive liquid crystal photonic crystal filter and a manufacturing method thereof. The filter comprises three glass substrates, wherein, one internal glass substrate is positioned in the middle and two external glass substrates are positioned at the two sides of the internal glass substrate; the inner sides of the two external glass substrates and the twosides of the internal glass substrate are plated with ITO electrode films which are alternately plated with multilayer films of silicon dioxide and titanium dioxide; the multilayer films are printed with oriented films which are subject to directional friction treatment and cause the friction directions of the opposite glass substrates to be antiparallel and overlaid; isolation pads used for controlling the thickness of the liquid crystal layers are arranged among all glass substrates and pure nematic phase liquid crystals with larger anisotropism difference of refractive index are injected among all glass substrates; the two liquid crystal layers are arranged in an antiparallel manner respectively and the arrangement directions of the two layers are vertical; and the peripheries of the glass substrates are provided with a sealing structure. The crystal filter and the manufacturing method have the advantages of low cost, low driving voltage, large tuning range, high tuning precision and good repeatability, being easy to control, and the like.

The invention discloses a method for quantitatively detecting a quail-derived component based on a ddPCR technology, and belongs to the field of molecular biological analysis. A sample to be detected is subjected to vacuum freeze drying to remove moisture and then ground into powder, a dried and uniform powder sample is obtained, DNA of the powder sample is extracted to serve as a template, the ddPCR primer pair and the probe of quails are added into an amplification reaction system, a microdroplet generator is used for generating microdroplets, a ddPCR instrument is used for amplification, after amplification is finished, the ddPCR instrument reads signals and analyzes data, and a quantitative detection result is obtained by contrasting a standard curve. According to the method, the sample freeze-drying treatment method can improve the uniformity of the sample, and meanwhile, the interference of the moisture content on quail-derived component quantification is removed; the ddPCR method is high in specificity and sensitivity, the quantitation limit is 5 mu g / mg, the detection limit is 1 mu g / mg, and quantitative detection work of quail-derived components in complex samples can be met.