Method for analyzing contents of effective substances in Cordyceps martialis fruiting body and residue by HPLC

Abstract

The invention discloses a method for analyzing the contents of adenosine and cordycepin in Cordyceps martialis fruiting body and solid culture medium residue thereof by HPLC. The method comprises the following steps of: firstly preparing standard solutions of adenosine and cordycepin and sample solutions of Cordyceps martialis fruiting body and solid culture medium residue thereof; and then detecting the sample solutions of Cordyceps martialis fruiting body and solid culture medium residue thereof respectively under the conditions that the chromatographic column is XBridge TMC 18 (5 mum, 4.6*150 mm), the mobile phase is methanol and ultra-pure water at a volume ratio of 10:90, the flow rate is 1.0 ml / min, the sample size is 10 muL (automatic sample injection), the column temperature is 30 DEG C, and the detection wavelength is 254 nm, wherein the sample solutions should appear chromatographic peaks having the same chromatographic retention time as the cordycepin standard solution. The method provided by the invention is convenient and effective, is particularly suitable for analyzing the contents of adenosine and cordycepin in Cordyceps martialis fruiting body and solid culture medium residue thereof, and has good reproducibility and stability.

Description

technical field [0001] The present invention relates to the field of analytical chemistry, in particular to a method for analyzing the content of adenosine and cordycepin in the fruiting body of Cordyceps militaris and its solid culture residue by HPLC method, and the method is particularly suitable for analyzing the artificially cultured fruiting body of Cordyceps militaris and its solid culture residue. Solid culture residue. Background technique [0002] Cordyceps militaris (also known as Cordyceps militaris, Cordyceps militaris) is a fungus of the genus Cordyceps ergotaceae ( Cordyceps militaris (L.) Link) is a rare and precious medicinal fungus unique to my country, which has multiple pharmacological activities such as organ expansion, sedation, anti-arrhythmia, blood pressure reduction, anti-pathogenic microorganisms, and anti-malignant tumors. Nucleoside components such as adenosine and cordycepin are used as the main reference indicators for the quality of Cordyceps...

AI Technical Summary

Problems solved by technology

At present, there is no unified national standard method for the determination of adenosine and cordycepin in Cordyceps militaris and its solid culture residues. Therefore, a simple and reliable method is established to analyze adenosine in fruiting bodies and their solid culture residues at the same time with one-time injection. The content of cordycepin has practical significance

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