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Method for preparing silkworm chrysalis polypeptide using complex enzyme for hydrolyzing silkworm chrysalis

A technology of compound enzymatic hydrolysis and silkworm chrysalis, applied in the direction of fermentation, can solve problems such as unsatisfactory effect, low yield of acid-soluble polypeptide, etc., and achieve the effects of appropriate hydrolysis reaction, many cutting sites and strong hydrolysis ability.

Inactive Publication Date: 2007-08-01
周维海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the compound plant enzymes or single enzymes they use are not ideal for hydrolyzing animal protein—silkworm chrysalis, and the yield of acid-soluble polypeptides is generally low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, get degreased silkworm chrysalis, first remove impurity and mildew, then take by weighing 5kg (its protein content is 62.3%), add 10kg clean water and carry out homogenization 3 times on the colloid mill, the slurry of gained passes through 100 mesh sieves Finally, take the filtrate, and use 35kg of clean water to make a filtrate containing 10% of silkworm chrysalis, then place these filtrates in a constant temperature stirring reactor, adjust the temperature to 55 ° C, and adjust the temperature with 1mol / L dilute hydrochloric acid or dilute caustic soda. The pH value is 7.0, add compound enzyme 25g, wherein bromelain (activity 60 * 10 4 Ug -1 ) 3.6g, neutral protease (activity 20×10 4 Ug -1 ) 21.4g, reacted for 3 hours, during which the pH was adjusted to be stable at 7.0 with dilute hydrochloric acid or dilute caustic lye. After the reaction, the temperature was raised to 85° C. to inactivate the enzyme for 20 minutes. After the hydrolyzate is coole...

Embodiment 2

[0025] Embodiment 2, get degreased silkworm chrysalis, first remove impurity and mildew, then take by weighing 1kg (its protein content is 71.6%), add 3kg clean water and carry out homogenization 3 times on the colloid mill, the slurry of gained passes through 100 mesh sieves Finally, take the filtrate, and use 196kg of clean water to make a filtrate containing 0.5% of silkworm chrysalis, then place these filtrates in a constant temperature stirring reactor, adjust the temperature to 51 ° C, and adjust the temperature with 1mol / L dilute hydrochloric acid or dilute caustic soda The pH value is 7.0, add compound enzyme 2g, wherein bromelain (activity 100 * 10 4 Ug -1 ) 0.4g, neutral protease (activity 25×10 4 Ug -1 ) 1.6g, reacted for 2.6 hours, during which the pH was adjusted to be stable at 7.0 with dilute hydrochloric acid or dilute caustic lye. After the reaction, the temperature was raised to 85° C. to inactivate the enzyme for 20 minutes. After the hydrolyzate is cool...

Embodiment 3

[0026] Embodiment 3, get degreased silkworm chrysalis, first remove impurity and mildew, then take by weighing 20kg (its protein content is 66.6%), add 40kg clean water and carry out homogenization 3 times on the colloid mill, the slurry of gained passes through 100 mesh sieves Finally, take the filtrate, and use 340kg of clean water to make a filtrate containing 5% of silkworm chrysalis, then place these filtrates in a constant temperature stirring reaction kettle, adjust the temperature to 60°C, and adjust the temperature with 1mol / L dilute hydrochloric acid or dilute caustic lye. The pH value is 7.0, add compound enzyme 40g, wherein bromelain (activity 40 * 10 4 Ug -1 ) 20g, neutral protease (activity 40×10 4 Ug -1) 20g, reacted for 3.5 hours, during which the pH was adjusted to be stable at 7.0 with dilute hydrochloric acid or dilute caustic lye. After the reaction, the temperature was raised to 85° C. to inactivate the enzyme for 20 minutes. After the hydrolyzate is c...

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PUM

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Abstract

The invention discloses a method for preparing silkworm chrysalis polypeptide by taking defatted silkworm chrysalis as raw material. It is characterized in that: it selects defatted silkworm chrysalis as raw material, takes composite enzyme of bromelain and neuter protease as proteolytic enzyme, carries out hydrolysis under special condition, and gets silkworm chrysalis polypeptide after drying. The invention is characterized by simple process, low production cost, long perservation time, ability of checking polypeptide content in silkworm chrysalis polypeptide through polypeptide content checking method, and the product is characterized by stable quality and ensured nutriment and effect. The product is widely used in food and health care food field, and can be matched with other animal, plant raw material with normal method to prepare capsule, soft capsule, granula, tablet and powder medicine.

Description

technical field [0001] The invention relates to a method for preparing extracts from animal bodies, in particular to a method for preparing silkworm chrysalis polypeptides from defatted silkworm chrysalis. Background technique [0002] Silkworm chrysalis (Silkworm Chrysalis) is the chrysalis of the silkworm (Bombyx mori Linnaeus) of the family Bombycidae or the chrysalis of the silkworm (Antheraea pernyi) and castor silkworm (Attacus cynthia ricini Boisduval) of the family Saturniidae. Analysis and determination show that the protein content in silkworm chrysalis is more than 50%, the oil content is 20% to 30%, and the rest is chitin and inorganic salts. The protein contained in 100 kilograms of silkworm chrysalis is equivalent to 85 kilograms of lean pork, or 96 kilograms. The protein contained in eggs and 109 kilograms of crucian carp, and silkworm chrysalis protein is a kind of complete protein, with a complete range of amino acids, including 18 kinds of amino acids, and ...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 周维海周维官
Owner 周维海
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