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Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof

A bioprocessing and ultrasonic technology, applied in the pulsed multi-frequency ultrasonic bioprocessing device and the application field of the deep processing of agricultural products, can solve the problems of not reaching resonance, contaminating the extract solution, difficult to excite the resonance frequency of the processing system, etc. Avoid the generation of standing waves, promote chemical reactions, and enhance the effect of destructive effects

Active Publication Date: 2010-05-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses a probe-type ultrasonic generator. The disadvantage of this generator is that it is only suitable for extracting materials that require high ultrasonic intensity and less processing in the laboratory; the surface of the probe will be corroded by cavitation, which will reduce the ultrasonic efficiency. Occurrence efficiency, cavitation corrosion products will fall into the volume, which will contaminate the extract solution; there is a problem of uneven distribution of the ultrasonic field in the liquid
For a complex bioprocessing system, it is difficult for a fixed ultrasonic frequency to excite the processing system to generate a resonant frequency that matches its own natural frequency, and cannot fully exert the effect of ultrasonic waves.
In principle, the current "sweep frequency" ultrasonic waves only have a wider vibration spectrum and more objects to act on, and cannot achieve the maximum resonance effect.

Method used

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  • Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof
  • Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof
  • Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof

Examples

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Effect test

Embodiment 1

[0046] Application of the present invention in the extraction of effective components of natural products—ultrasound-assisted extraction of Porphyra zebra protein. 1 kg of dry laver (containing 41.3% protein) was pulverized to 60 mesh, mixed with 40 L of water, and added to an ultrasonic treatment tank. Put two transducers with a frequency of 28kHz at the bottom of the treatment tank, and place two transducers with a frequency of 20kHz close to the liquid surface (about 20cm apart). The input power of each transducer is set to 600W (total power of 4 transducers is 2400W), the sweep period is 20ms, the sweep width is ±2kHz, the ultrasonic time of pulse ultrasound is 100s, the rest time is 3s, and the cycle pump is set by the computer. The flow rate is 5L / min, and the initial temperature of the extract is 45°C. Ultrasound-assisted extraction was stopped after 80 min. The extract was centrifuged at 3000 r / min for 10 min, and the supernatant was taken to measure the protein cont...

Embodiment 2

[0049] Application of the present invention in the extraction of active ingredients of natural products—ultrasound-assisted extraction of rapeseed protein. 1.8 kg of rapeseed meal crude protein (containing 48% protein) was pulverized to 90 mesh and mixed with 30 L of water, then added to an ultrasonic treatment tank, and the pH was adjusted to 9.8. Put two transducers with a frequency of 28kHz at the bottom of the treatment tank, and place two transducers of 24kHz close to the liquid surface (about 15cm apart). The input power of each transducer is set to 600W (total power of 4 transducers is 2400W), the sweep period is 18ms, the sweep width is ±2kHz, the ultrasonic time of pulse ultrasound is 50s, the rest time is 5s, and the cycle pump is set by the computer. The flow rate is 16L / min, and the initial temperature of the extract is 30°C. Ultrasound-assisted extraction was stopped after 30 min. The extract was centrifuged at 3200 r / min for 10 min, and the supernatant was take...

Embodiment 3

[0052]The application of the present invention in the extraction of effective components of natural products—ultrasound-assisted synchronous extraction of rice bran protein and polysaccharides. Mix 200g of defatted rice bran and 20L of water and add them into the ultrasonic treatment tank. Two transducers with frequencies of 24kHz and 64kHz are placed in the bottom of the treatment tank. The input power of each transducer is set to 600W, the sweep period is 36ms, the sweep width is ±2kHz, the ultrasonic time of pulse ultrasound is 200s, the rest time is 5s, the flow rate of the circulating pump is 2L / min, and the extraction liquid is set by the computer. The initial temperature was 45°C. Ultrasound-assisted extraction was stopped after 80 min. The extract was centrifuged at 3000 r / min for 10 min, and the supernatant was taken to measure the protein content, and the total extraction rate of protein and polysaccharide was calculated to be 86.2%.

[0053] Controlled experiment...

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Abstract

The invention discloses a device and a method for biological processing with pulsed multi-frequency ultrasonic waves in a sweep-frequency mode and use thereof, which relate to the field of agricultural product deep processing. The device consists of an ultrasonic wave processing system, an ultrasonic wave generating system and a computer control system, which are sequentially connected by cables, wherein the ultrasonic wave processing system consists of an upper ultrasonic wave transducer, a lower ultrasonic wave transducer, an ultrasonic wave processing tank body, a circulating pump and an upper oscillatory plate height adjusting screw rod; an ultrasonic wave generator set consists of a group of pulsed ultrasonic wave generators at different frequencies; and the control system consists of an ultrasonic wave controller, a temperature controller and a computer. The equipment can be used in ultrasonic wave-assisted extraction of active ingredients of natural products and the extraction efficiency is improved 10 to 90 percent. The equipment can be used in ultrasonic wave-assisted enzymolysis of biological macromolecules, wherein the ultrasonic wave-assisted enzymolysis includes preprocessing raw materials, preprocessing hydrolases and performing enzymolysis in an ultrasonic wave field; and the enzymolysis efficiency is improved by 25 to 65 percent.

Description

technical field [0001] The invention relates to the field of intensive processing of agricultural products, in particular to a pulsed multi-frequency ultrasonic biological processing device in a frequency sweep mode and its application in the field of intensive processing of agricultural products. Background technique [0002] Usually, waves with a frequency of 20kHz to 10MHz are called ultrasonic waves, which are elastic mechanical waves propagating in the medium. As a physical means and tool, ultrasonic technology can produce a series of extreme conditions in the medium commonly used in chemical reactions. Such as local and instantaneous high temperature, high pressure, etc., this energy can not only stimulate or promote chemical reactions, speed up chemical reactions, and even change the direction of certain chemical reactions. In recent years, it has been widely used in the extraction and separation of active ingredients of natural products, the preparation of bioactive ...

Claims

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Application Information

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IPC IPC(8): B01J19/10B01D11/02
Inventor 马海乐骆琳何荣海王振斌任晓锋
Owner JIANGSU UNIV
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