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Paenibacillus polymyxa and difenoconazole compound fungicide and application thereof

A technology for compounding fungicides and Paenibacillus polymyxa, which is applied in the direction of fungicides, applications, biocides, etc., can solve the problem of not finding Paenibacillus polymyxa, etc., to delay the emergence of bacterial resistance, and the environment is pollution-free and good The effect of antibacterial effect

Inactive Publication Date: 2014-03-12
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no related compound fungicides of Paenibacillus polymyxa (P.polymyma) and difenoconazole have been found

Method used

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  • Paenibacillus polymyxa and difenoconazole compound fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 : 5% difenoconazole + 2.5*10 7 For the preparation of cfu / ml Paenibacillus polymyxa wettable powder, the distribution ratio of each component is:

[0043] The mass ratio of 95% difenoconazole technical substance is 5.26%,

[0044] The concentration of Paenibacillus polymyxa is 10*10 7 cfu / ml bacterial solution 25%,

[0045] Wetting agent: sodium dodecylbenzenesulfonate is 5% by mass,

[0046] Dispersant: sodium lignosulfonate is 5% by mass,

[0047] Filler: diatomaceous earth is 59.74%.

[0048] Mix the Paenibacillus polymyxa fermentation broth with a certain amount of crushed diatomaceous earth, fully dry the mixture and difenoconazole original drug, sodium dodecylbenzenesulfonate, sodium lignosulfonate, etc., cool and mix , Airflow crushing and fine grinding can be the product.

Embodiment 2

[0049] Example 2 : 3% Difenoconazole + 2*10 7 For the preparation of cfu / ml Paenibacillus polymyxa granules, the distribution ratio of each component is:

[0050] The mass ratio of 95% difenoconazole technical substance is 3.16%,

[0051] The concentration of Paenibacillus polymyxa is 10*10 7 cfu / ml bacteria powder 20%,

[0052] Wetting and dispersing agent: sodium dodecylbenzenesulfonate is 1% by mass,

[0053] Binder: polyvinyl acetate is 1% by mass,

[0054] Auxiliary disintegration agent: urea is 2% mass ratio,

[0055] Adsorbent: white carbon black 2% mass ratio,

[0056] Stabilizer: butyl glycidyl ether is 7% by mass,

[0057] Carrier: 63.84% activated clay.

[0058] Fully mix the original drug, bacterial liquid, sodium dodecylbenzenesulfonate, polyvinyl acetate, urea, white carbon black, butyl glycidyl ether, and activated clay, ultrafinely pulverize, granulate, dry, and sieve. Grading is the product.

Embodiment 3

[0059] Example 3 : 8% Difenoconazole + 1*10 7 The preparation of cfu / ml Paenibacillus polymyxa water-dispersible granules, the distribution ratio of each component is:

[0060] The mass ratio of 95% difenoconazole technical substance is 8.42%,

[0061] The concentration of Paenibacillus polymyxa is 10*10 7 cfu / ml bacterial powder 10%,

[0062] Wetting agent: sodium lignosulfonate is 8% by mass,

[0063] Binder: polyvinyl acetate is 9% by mass,

[0064] Auxiliary disintegrant: urea is 15% mass ratio,

[0065] Stabilizer: butyl glycidyl ether is 4% by mass,

[0066] Carrier: activated clay is 45.58%.

[0067] Each component is superfinely pulverized, then granulated, dried, sieved, and classified to obtain the product.

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Abstract

The invention provides a compound fungicide. The compound fungicide comprises main agents and auxiliary agents, wherein the main agents include difenoconazole and paenibacillus polymyxa, difenoconazole accounts for 1-80% of the total compound fungicide by weight, and the addition of paenibacillus polymyxa is 1*10<6>-1*10<8>cfu per milligram of compound fungicide. The compound fungicide has the advantages that the fast-acting property of the microbial fungicide can be improved and the pesticide residues can be reduced by adopting the biocontrol bacterium and chemical fungicide mixture, so that the compound fungicide meets the requirement of sustainable development and the requirements of current agricultural production, is environment-friendly and residue-free, is beneficial to delaying generation of drug resistance of germs, and especially has good control effects on blight, caused by fusarium oxysporum, of orchids such as cymbidium sinense, cymbidium ensifolium and the like.

Description

technical field [0001] The invention relates to a compound fungicide of bacteria and compounds, more specifically, to a compound fungicide of Paenibacillus polymyxa and difenoconazole and application thereof. Background technique [0002] Paenibacillus (Paenibacillus) is a new genus established by Ash et al. in 1993, including 11 species of the original genus Bacillus, and its type species is Paenibacillus polymyxa. Studies have shown that Paenibacillus polymyxa can produce peptides, proteins, nucleosides and other metabolites with antibacterial effects, which can prevent and control various plant diseases. Kavitha et al. (2005) isolated a protein with a molecular weight of 37KDa and high temperature resistance from the fermentation broth of Paenibacillus polymyxa VLB16, which can inhibit Magnaporthe oryzae and Rhizoctonia solani bacteria silk grows. Sun Changpo's research found that P. polymyma Cp_S316 has strong antagonistic effects on 8 kinds of pathogenic fungi and 4...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01N43/653
Inventor 程东美向梅梅张云霞施祖荣
Owner ZHONGKAI UNIV OF AGRI & ENG
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