51 results about "Cymbidium goeringii" patented technology

The invention provides a method for culturing and rapidly propagating the stem tip tissue of a rare cymbidium goeringii. By taking the stem tip of a bud of the rare cymbidium goeringii as an explants, the method comprises the following steps: inducing rhizome from the stem tip, performing successive multiplication on the rhizome, inducing the bud from the rhizome, rooting and strengthening the seedling, transplanting the test-tube plantlet and the like; an optimal culture medium is chosen at the stages of cutting and sterilizing the bud, stripping the stem tip, inducing the rhizome, multiplying, bud differentiation, rooting and strengthening and the like, so that quick multiplication of the seedling, high yield, good quality and low cost of the rare cymbidium goeringii can be realized to lay the foundation for the industrial production of the cymbidium goeringii. The method of the invention has high operability, well solves the problems of slow multiplication speed, easy degeneration and the like in a traditional division method of the cymbidium goeringii, can gradually realize the industrial production of the cymbidium goeringii species of high commodity value and has high application and popularization value.

The invention provides a method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings, which comprises the following steps of: performing artificial pollination to obtain hybrid seeds; picking off the capsule when the capsule has not reached full maturity; disinfecting the surface of the capsule and aseptically sowing the capsule; culturing the capsule in the dark and sprouting to obtain the rhizome; inoculating the rhizome into a bud induction culture medium; inducing bud through the rhizome, rooting and culturing the sprout; and transplanting test-tube plantlet and the like. The method ensures high germination of the cymbidium goeringii hybrid seeds, short period for raising the seedlings and good quality of the seedlings by using three culture media optimized by sifting. The complete set of method of the invention has high operability, well solves the problems of extremely difficult germination of the cymbidium goeringii hybrid seeds under natural conditions due to the incomplete embryonic development and has high application value and popularization value.

The invention relates to a method of seedling with tissue culturing by using spring orchid seed, comprising the following steps: taking the capsule of not broke up from spring orchid as explant; immersing with alcohol or wiping it with absorbent cotton dipped with alcohol, cutting up the capsule to fetch out the pulverulent seed, loading it into sheeting bag, immersing it in the alcohol, putting it in the NaOH solution with concentration being 0.08-0.12mol / l for 8-10 minutes, putting it into sodium hypochlorite solution of 10.5í‚2% for 6-8 minutes; sowing the treated seeds on the culturing medium for seed germination, lighting 14-16 hours fir every day, with the luminous intensity being 1000-1500lux, temperature 23í‚2 Deg C, culturing for 160-180 days, and growing out original bulb; transferring the original bulb to the culturing medium for seed germination the same with mentioned above for successive transfer culture, for 50-60 days, and growing out new sprout or original bulb, cutting the original bulb continuously for successive transfer culture and reaching the geometric extension. The invention employs method of tissue culturing by using spring orchid seed, to breed species of high quality and to choose orchid plant from seed seedling.

The invention discloses a CgWRKY57 encoding gene of cymbidium goeringii, and application of the encoding gene. The nucleotide sequence of the CgWRKY57 gene is shown in SEQ ID NO. 1, the amino acid sequence of the expressed protein of the CgWRKY57 gene is shown in SEQ ID NO. 2. By cloning and identifying the CgWRKY57 gene of 'Song Mei' which is a cultivated variety of the cymbidium goeringii, analyzing the gene expression and verifying the functions of the gene, an arabidopsis thaliana plant with the overexpressed CgWRKY57 gene is found to have phenotypes such as growth retardation and plant dwarf; therefore, the gene has a broad application prospect in the production and breeding of the cymbidium goeringii and other plants.

This invention discloses an aroma-producing compound from Cymbidium goeringii and the extraction method thereof. The compound comprises octadecatrienoic aldehyde,nerolidol,dibutylphthalate,heptacosane,pentadecanoic acid,pentadecanoic acid,hexadecanoic acid(palmic acid),oleic acid,linolic acid and cyclohexadecane. The aroma-producing compound is extracted from the flowers of Cymbidium goeringii by the following steps: leaching and filtering, reflux leaching, filtering, combined seperation, volatilized speration, concentration and drying. The main discovery is the octadecatrienoic aldehyde, a critical aroma-producing substance. Besides, the extraction rate of the aroma-producing substances is pretty high, with the content upto 30% by using octadecatrienoic aldehyde and nerolidol as the major aroma-producing product. The components of this invention has satisfied the basic requirements as natural flavoring, and can be used in flavoring agents, perfume, scent emitting objects and bath articles, allowing this natural perfume for industrial application.

The invention discloses a cymbidium goeringii CgWRKY75 coding gene and application thereof. The nucleotide sequence of the CgWRKY70 gene is shown as SEQ ID NO.1, and the amino acid sequence of an expression protein is shown as SEQ ID NO.2. Through cloning and identification, gene expression analysis and functional verification of the CgWRKY70 gene of a cultivated cymbidium goeringii variety Songmei, the invention discovers that: in a seedling stage of CgWRKY70 transgenic Arabidopsis thaliana, compared with wild Arabidopsis thaliana WT, the CgWRKY70 transgenic Arabidopsis thaliana has larger leaves than the wild Arabidopsis thaliana, has yellowish leaves, the flowering is delayed, and the CgWRKY70 is shown to promote the flowering of transgenic plant, so that the CgWRKY70 gene may have wideapplication in orchid flower characteristic improvement.

The invention discloses a cymbidium goeringii miR390a gene and application of the cymbidium goeringii miR390a gene for controlling plant root system development. The precursor sequence segment encoding gene of the miR390a is obtained from a cymbidium goeringii cultivated variety "Song Mei", expression analysis is carried out in the cymbidium goeringii, then, the precursor sequence segment encodinggene is constructed to an overexpression vector, the overexpression vector is imported into an objective plant to verify the function of the overexpression vector so as to discover the phenotypes ofan arabidopsis thaliana plant that an overexpression miR390a gene has a high root system growth speed in a seedling stage and a euphylla formation speed is quickened, and therefore, the gene has a wide purpose in the production and the seed breeding of orchids and other horticultural plants.

The invention discloses cymbidium goeringii miR159b gene and application thereof in enhancing cold sensitivity of plants. The method comprises the following steps: obtaining a precursor sequence fragment encoding gene MIR159b of miR159b from a cymbidium goeringii cultivated variety 'Song mei', and performing expression analysis in cymbidium goeringii, constructing the precursor sequence fragment encoding gene MIR159b into an overexpression vector, introducing the overexpression vector into a target plant to verify the function, screening and culturing to obtain a T3-generation plant, wherein it is found that the root growth speed in the seedling stage is obviously higher than that of a wild type plant; after transplantation of the wild type and 35S: MIR159b transgenic plants into soil for60 days, carrying out low-temperature stress treatment at 4 DEG C, it is found that phenotypes such as main inflorescence sagging, non-standing, stem leaf sagging and curling, basal leaf yellowing andthe like of the transgenic plants are caused, so that the gene has wide application in production of orchids and other horticultural plants.

The invention discloses a special culture medium for cymbidium goeringii. The special culture medium is prepared from the following substances in parts by weight: 70-80 parts of an MS culture medium, 15-20 parts of ferrous sulfate, 6-8 parts of sugarcane, 6-8 parts of glucose, 8-10 parts of lactoalbumin hydrolysate, 8-12 parts of corn endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut milk, 3-5 parts of indoleacetic acid, 3-6 parts of indolebutyric acid, 2-5 parts of naphthylacetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isopentenylamino purine, 1-3 parts of gibberellin, 20-30 parts of agar, 5-15 parts of activated carbon and 40-50 parts of water. The special culture medium for the cymbidium goeringii is prepared according to the plant characteristics of the cymbidium goeringii, is rich in nutrition, is capable of effectively improving the survival rate of the cymbidium goeringii and has excellent market prospect.

The invention relates to the technical field of planting materials of orchids, and specifically relates to a planting material for improving the growth of orchids. The formula of the planting materialincludes the following components: in parts by mass, 30-45 parts of bark, 30-40 parts of peanut shells, 2-5 parts of orchid cultivating stone, 3-8 parts of plant ash, 8-15 parts of vesuvianite, 3-8 parts of vermiculite, 3-8 parts of Quercus spinosa David ex Fr. Leaves and 5-10 parts of potassium humate. The invention also discloses a preparation method of the planting material for promotion of the growth of orchids. Through optimization of the formula and the preparation method, good promotion effects on stages of the orchid growth are achieved through the prepared planting material, good effects of water permeability and air permeability are achieved, and the fertilizer effect can last for a long period of time; the prepared planting material has good application effects on different types of orchids, and the application effects on Cymbidium goeringii, Cymbidium faberi, Cymbidium ensifolium, Cymbidium sinense and Cymbidium kanran are more significant; and meanwhile, the adopted raw materials of the planting material is simple, and fast seedling emergence, small dosage of the planting material and cost saving are achieved during growth of the orchids.

The invention discloses a cymbidium goeringii CgWRKY4 gene and an application thereof. A nucleotide sequence of the CgWRKY4 gene is as shown in SEQ ID NO.1; and an amino acid sequence of expression protein of the CgWRKY4 gene is as shown in SEQ ID NO.2. Through cloning and identification, gene expression analysis and function verification of a cymbidium goeringii cultivar ''songmei'' CgWRKY4 gene,it is found that CgWRKY4 transgenic arabidopsis has the advantages that a CgWRKY4 transgenic plant is small and yellowish in leaf and delayed in flowering stage in comparison with wild arabidopsis WTat a seedling stage, and it is proved that the gene can inhibit nutritional growth of the plant and cause nutritional deficiency to delay flowering. Therefore, the gene may have a broad application in the aspect of flower character improvement of cymbidium.

The invention relates to cucumber essence and a preparation method thereof. The cucumber essence is prepared by adding green fragrance base materials prepared from green fragrance flavor materials generated by guava jam and sacha inchi oil with cymbidium goeringii leaf residues and cymbidium goeringii juice into green cucumber juice. The cucumber essence has natural green cucumber flavor and freshenough taste, presents unique fragrance of green cucumbers, and can provide a vivid sensory experience for consumers.

The invention relates to a cymbidium goeringii rhizome growing method, and belongs to the field of plant biotechnologies. The cymbidium goeringii rhizome growing method comprises the four steps of material preparation, pretreatment, cultivation and management and protection. An appropriate planting pot, pot soil and cymbidium goeringii are selected, the corm of the cymbidium goeringii makes direct contact with the pot bottom of the planting pot in the planting process, then management and protection for about two months are conducted, and cymbidium goeringii rhizome growth can be seen on the corm of the cymbidium goeringii. The cymbidium goeringii rhizome growing method overcomes the defect that the rhizome obtained through seed germination cannot completely inherit the excellent characteristics of the male and female parent in the prior art, the method also overcomes the defects that when stem tip induction is used for obtaining the rhizome in the prior art, cultivation difficulty is large and time is long, hence, it is guaranteed that the grown rhizome can completely inherit the excellent characteristics of the male and female parent, the rhizome can be grown out quickly and conveniently, and the method is suitable for industrialization production of factories.

The invention discloses a special culture medium for cymbidium goeringii var. serratum. The special culture medium is prepared from the following substances in parts by weight: 70-80 parts of an SH culture medium, 8-10 parts of EDTA disodium, 6-8 parts of sugarcane, 6-8 parts of glucose, 8-10 parts of lactoalbumin hydrolysate, 8-12 parts of corn endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut milk, 3-5 parts of indoleacetic acid, 3-6 parts of indolebutyric acid, 2-5 parts of naphthylacetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isopentenylamino purine, 1-3 parts of gibberellin, 20-30 parts of agar, 5-15 parts of activated carbon and 40-50 parts of water. The special culture medium for the cymbidium goeringii var. serratum is prepared according to the plant characteristics of the cymbidium goeringii var. serratum, is rich in nutrition, is capable of effectively improving the survival rate of the cymbidium goeringii var. serratum and has excellent market prospect.

The invention discloses a pith approach-grafting method for inflorescence of Cymbidium goeringii; the method comprises: (1) storing inflorescence; (2) preparing inflorescence scions; (3) treating the inflorescence scions with hormone; (4) preparing inflorescence stocks; (5) grafting; (6) treating graft unions; (7) managing water content of grafts; (8) managing lighting for the grafts. It is verified for the inflorescence of Cymbidium goeringii cultivated by the above method that grafting survival rate of Cymbidium goeringii reaches 100%, flowering rate reaches 100%, early flowering occurs on day 5 to day 9 after grafting, the flowering phase includes 25-35 days, the problem of adjusting the flowering phase of Cymbidium goeringii is solved, and the method has important actual application value.

The invention discloses a technology for planting Zhejiang Cymbidium goeringii (Reichb.F.) Reichb. F. with a light growing medium. According to a combined growing medium method, the growing medium with air-permeable transport, good water retention and light texture is adopted, rapid rooting of the planted cymbidium can be carried out, and new roots can be generated within 10 days at an air temperature of 20-30 DEG C, so that the cymbidium grows rapidly, the germination rate and efficiency are improved, and second pot turning for replacement of the growing medium is not needed within 2 years. The technology is suitable for indoor planting of Zhejiang Cymbidium goeringii (Reichb.F.) Reichb. F., has no special requirements for watering and fertilization, and a traditional cymbidum growing technique with requirements of watering is avoided. The technology is more suitable for cymbidium growing requirements of modern young people, the cymbidium market is expanded, the growing medium is light, and has good clustering properties, and the technology is suitable for express delivery needs of network sales.

The invention belongs to the technical field of agricultural pesticide fertilizers and particularly relates to a field-cultivated cymbidium goeringii soil-improvement pesticide fertilizer with a function of soil insect control and an application method of the field-cultivated cymbidium goeringii soil-improvement pesticide fertilizer. The field-cultivated cymbidium goeringii soil-improvement pesticide fertilizer is composed of a main ingredient and an auxiliary ingredient by a weight part ratio of 1000 to 1-50; the main ingredient is composed of saw dust and either pine barks or pine needles by a weight part ratio of 1 to 9-9:1; the auxiliary ingredient comprises a component with a bactericidal function. By adoption of the field-cultivated cymbidium goeringii soil-improvement pesticide fertilizer, disease resistance of cymbidium goeringii can be improved to increase survival rate of the field-cultivated cymbidium goeringii. In addition, the field-cultivated cymbidium goeringii soil-improvement pesticide fertilizer is low in cost, saw dust in solid wood factories can be recycled, pesticide consumption is reduced, and planting cost of the field-cultivated cymbidium goeringii is reduced.

The invention discloses a method for in-vitro conservation and growth recovery after conservation of a germplasm resource of cymbidium goeringii. The method comprises the steps: picking off stem tips from newly grown pseudobulbs of cymbidium goeringii, culturing the stem tips on the surface of a protocorm induction culture medium to form protocorms, and carrying out repeated cutting and secondary culture on the protocorms to reproduce a required amount of protocorms; then, culturing the protocorms in an in-vitro conservation culture medium, and carrying out dark culture on the protocorms under the condition of 0-5 DEG C after the protocorms enter a development and growth stage; and transferring the protocorms in dark culture to a differential culture medium, and differentiating the protocorms to produce young seedlings. The protocorms are induced by using a cymbidium goeringii stem tip culture technology and are then differentiated to obtain the young seedlings of cymbidium goeringii. Due to the combination of conserving the protocorms at low temperature and adding a proper amount of paclobutrazol, the conservation time can be greatly prolonged, the secondary culture frequency can be reduced, and the hereditary character of the germplasm resource can be effectively remained. If the germplasm resource is cultured under normal temperature and light conditions after being conserved for a certain time, growth can be rapidly recovered, and the young seedlings can be differentiated.

The invention discloses an aroma composition extracted from spring orchids and an extraction method thereof. The composition includes octadecatrienal, nerolidol, dibutyl phthalate, heptacosanol , Pentadecanoic acid, hexadecanoic acid (palmitic acid), oleic acid, linoleic acid and cyclohexadecane were extracted by leaching and filtering, reflux leaching, filtering, merging and separation, volatile separation, concentration and drying steps, and successfully The aroma substance has been extracted from the flowers of Chunlan, the most important thing is that the main aroma substance octadecatrienal has been found, and the extraction rate of the aroma substance of the present invention is also relatively high. Octatrienal and nerolidol are the main components of the fragrance, and their content is almost 30%. The composition of the present invention has met the basic requirements of natural fragrances, and can be used in fragrances, perfumes, fragrances, etc. according to existing methods. In fragrance body and bath products, this natural "orchid fragrance" can be applied in industry.

The invention discloses a cymbidium goeringii CgWRKY65 coding gene and application thereof. The nucleotide sequence of the CgWRKY65 gene is shown as SEQ ID NO.1, and the amino acid sequence of an expression protein is shown as SEQ ID NO.2. Through cloning and identification, gene expression analysis and functional verification of the CgWRKY65 gene of a cultivated cymbidium goeringii variety Songmei, the invention discovers that: in a seedling stage of CgWRKY65 transgenic Arabidopsis thaliana, compared with wild Arabidopsis thaliana WT, the whole CgWRKY65 transgenic plant is short, has yellowish leaves, and has a tendency of premature senescence, so that the CgWRKY65 gene may have wide application in orchid nutrition and reproduction improvement.

The invention discloses a household Cymbidium goeringii maintenance APP based on big data and image recognition. The APP is characterized in that after a user logs in the APP, stem, leaf and flower parts of Cymbidium goeringii are scanned through utilization of a camera; the APP automatically sends image data and geographical location data of a Cymbidium goeringii sample to a cloud server; the cloud server judges that the sample is the Cymbidium goeringii and judges the growth condition and the disease and pest condition of the Cymbidium goeringii through adoption of an image recognition technology; data of the Cymbidium goeringii of which geographical location is near to that of the sample and growth condition is similar to that of the sample is called from a Cymbidium goeringii big database for comparison, a maintenance method for the sample Cymbidium goeringii is obtained and the method is pushed to the APP. Through application of the mode, according to the APP, a user without any Cymbidium goeringii maintenance knowledge is enabled to analyze the growth condition of the planted Cymbidium goeringii rapidly and precisely through simple scan operation with the help of the APP, and the user is instructed to take scientific and reasonable disease and pest control and daily maintenance measures.

The invention discloses a spring organic fertilizer for cymbidium goeringii and a preparation method thereof. The spring organic fertilizer comprises the following components in parts by mass: 12 to 14 parts of rice hull powder, 10 to 15 parts of urea, 20 to 25 parts of calcium superphosphate, 10 to 12 parts of weathered coal, 23 to 25 parts of straw, and 200 to 300 parts of water. The spring organic fertilizer for cymbidium goeringii cannot cause soil hardening, does not need topdressing after single fertilization, and can effectively improve the soil structure.

The invention discloses a medium used for Cymbidium goeringii tissue culture, and relates to the technical field of flower tissue culture. 2,4-Dichlorophenoxyacetic acid, palm oil, citric acid and other substances are added to a basal medium to make the medium meet nutrients, the pH value and other parameters needed by protocorm induction in order to make the success rate of protocorm formation reach 88.5%, and nutrient components and the ratio of the above obtained enrichment medium and a rooting and seedling strengthening medium are adjusted in order to improve the survival rate of Cymbidium goeringii and make the survival rate reach 91% or above.

The invention discloses a method for promoting germination of cymbidium goeringii seeds. The method comprises the following steps of disinfecting cymbidium goeringii capsules; placing seeds in a sealed sterilization container, adding a nutrient solution and Tween 80, performing horizontal oscillation, and vertically standing after oscillation to obtain a treatment solution A; taking out the seeds from the treatment solution A, placing the seeds in the sealed sterilization container, adding a culture solution, and performing horizontal oscillation to obtain a treatment solution B; performing negative pressure pumping treatment on the treatment solution B, and discharging bubbles of seed coats to obtain a treatment solution C; and pouring the treatment solution C into a culture bottle for seed culture. The Tween 80 and the nutrient solution are matched to treat seed coats, the Tween 80 can effectively improve the non-hydrophilicity of the seed coats, contact between the nutrient solution and the seed coats is improved, and the watertightness of the seed coats is improved; and then the seeds are placed in the culture solution and subjected to negative pressure treatment, the bubbles of the seed coats are discharged, the culture solution synchronously enters the seed coats and makes full contact with embryonic cells, and seed germination is effectively promoted.

The invention relates to a tea making method, and concretely relates to a cymbidium goeringii flower green tea production method. The method is characterized in that 5 kilograms of cymbidium goeringiiflowers are taken and placed in an aluminum pot, liquid nitrogen is added to cause the cells of cymbidium goeringii flowers to rupture, a filter screen is arranged in the middle of the aluminum pot;10 kilograms of finished green tea is taken, the water content is less than 5%, and the finished green tea is placed on the filter screen; the green tea is covered with 5 kilograms of cymbidium goeringii flowers, a food grade desiccant bag is placed outside the aluminum pot, and the aluminum pot and desiccant bag are covered with a large container lid for standing for 24 hours; after 24 hours, thefilter screen is directly taken and the cymbidium goeringii flowers are seperated; after separating the cymbidium goeringii flowers, the green tea is mixed with 5 kg of fresh cymbidium goeringii flowers and placed in an enclosed space, the food grade desiccant bag is placed, and an ultrasonic generator is set, the ultrasonic frequency of an ultrasonic device is 25KHz to 50KHz, and the treatment is carried out for 24 hours. The technology adopts the method of rupturing cells by liquid nitrogen, and uses ultrasonic vibration to fully absorb the cymbidium goeringii flowers into the fragrant substance in the gap of green tea.

The invention discloses cymbidium goeringii miR159b gene and application thereof in enhancing cold sensitivity of plants. The method comprises the following steps: obtaining a precursor sequence fragment encoding gene MIR159b of miR159b from a cymbidium goeringii cultivated variety 'Song mei', and performing expression analysis in cymbidium goeringii, constructing the precursor sequence fragment encoding gene MIR159b into an overexpression vector, introducing the overexpression vector into a target plant to verify the function, screening and culturing to obtain a T3-generation plant, wherein it is found that the root growth speed in the seedling stage is obviously higher than that of a wild type plant; after transplantation of the wild type and 35S: MIR159b transgenic plants into soil for60 days, carrying out low-temperature stress treatment at 4 DEG C, it is found that phenotypes such as main inflorescence sagging, non-standing, stem leaf sagging and curling, basal leaf yellowing andthe like of the transgenic plants are caused, so that the gene has wide application in production of orchids and other horticultural plants.

The invention discloses a pith approach-grafting method for inflorescence of Cymbidium goeringii; the method comprises: (1) storing inflorescence; (2) preparing inflorescence scions; (3) treating the inflorescence scions with hormone; (4) preparing inflorescence stocks; (5) grafting; (6) treating graft unions; (7) managing water content of grafts; (8) managing lighting for the grafts. It is verified for the inflorescence of Cymbidium goeringii cultivated by the above method that grafting survival rate of Cymbidium goeringii reaches 100%, flowering rate reaches 100%, early flowering occurs on day 5 to day 9 after grafting, the flowering phase includes 25-35 days, the problem of adjusting the flowering phase of Cymbidium goeringii is solved, and the method has important actual application value.

The invention discloses a cymbidium goeringii miR159a gene and an application of the cymbidium goeringii miR159a gene in accelerating plant life cycle. An miR159a precursor sequence fragment coding gene is obtained from cymbidium goeringii cultivar ''Song Mei'', expression analysis is performed in the cymbidium goeringii, then the gene is constructed into an overexpression vector and introduced into a target plant to verify the function, and results find that overexpression miR159a gene arabidopsis thaliana plants have a shortened juvenile period, an advanced flowering period, advanced fruit ripening and other phenotypes that accelerate the life cycle, so that the gene has wide applications in the production and breeding of orchids and other horticultural plants.