Method for in-vitro conservation and growth recovery after conservation of germplasm resource of cymbidium goeringii
An in-vitro preservation and resource technology, applied in the field of plant tissue culture, can solve the problems of large manpower and material resources, cross-contamination, genetic variation, etc., and achieve the effects of prolonging the preservation time, strong regeneration ability, and stable genetic genes.
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Embodiment 1
[0026] A method for in vitro preservation of Chunlan germplasm resources and recovery of growth after preservation, the specific steps are as follows:
[0027] (1) The shoot tip was stripped from the newly grown pseudobulb of Chunlan, and placed on the surface of the protocorm induction medium supplemented with 0.5 mg / L 6-benzyl adenine in VW medium to cultivate protocorms.
[0028] Among them, the stripping method of the stem tip is as follows: select a pseudobulb with a length of 5 cm, wash the dust on the surface with tap water, peel off the outermost leaf, scrub it with alcohol with a volume concentration of 75% for 3 seconds, and put it in a concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 3 times, dried in the air or blotted the surface moisture with filter paper, and stripped the shoot tips with a size of about 0.5 mm under a microscope.
[0029] Place the shoot tip on the surface of the protocorm induction medium in the te...
Embodiment 2
[0043] A method for in vitro preservation of Chunlan germplasm resources and recovery of growth after preservation, the specific steps are as follows:
[0044] (1) The shoot tip was stripped from the newly grown pseudobulb of Chunlan, and placed on the surface of the protocorm induction medium supplemented with VW medium supplemented with 1 mg / L 6-benzyl adenine to cultivate protocorms.
[0045] Among them, the stripping method of the stem tip is as follows: select a pseudobulb with a length of 7 cm, clean the surface dust with tap water, peel off the two outermost leaves, scrub with alcohol with a volume concentration of 75% for 4 seconds, and put in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 4 times, dried in the air or blotted the surface moisture with filter paper, and stripped the shoot tips with a size of about 0.5 mm under a microscope.
[0046] Place the shoot tip on the surface of the protocorm induction medi...
Embodiment 3
[0058] A method for in vitro preservation of Chunlan germplasm resources and recovery of growth after preservation, the specific steps are as follows:
[0059] (1) The shoot tip was stripped from the newly grown pseudobulb of Chunlan, and placed on the surface of the protocorm induction medium supplemented with 0.8mg / L 6-benzyl adenine in VW medium to cultivate protocorms.
[0060] Among them, the stripping method of the stem tip is as follows: select a pseudobulb with a length of 6 cm, clean the surface dust with tap water, peel off one leaf of the outermost layer, scrub it with alcohol with a volume concentration of 75% for 4 seconds, and put it in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 3 times, dried in the air or blotted the surface moisture with filter paper, and stripped the shoot tips with a size of about 0.5 mm under a microscope.
[0061] Place the shoot tip on the surface of the protocorm induction mediu...
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