Establishment of a high-frequency regeneration system for Sinus cuspidatum based on callus induction

A technology of callus induction and high-frequency regeneration, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of slow propagation speed of ramets, low multiplication coefficient, and inability to meet the needs of factory seedlings

Active Publication Date: 2021-07-16
HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES +1
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  • Abstract
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Problems solved by technology

[0004] The present invention aims at optimizing the regeneration system, and takes maintaining genetic stability as the core, and provides a method for establishing a high-frequency regeneration system based on callus induction, so as to solve the problem of slow propagation speed of ramets, low multiplication coefficient, and brown seedlings in tissue culture. Problems that cannot meet the needs of factory seedlings such as vitrification and vitrification

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  • Establishment of a high-frequency regeneration system for Sinus cuspidatum based on callus induction
  • Establishment of a high-frequency regeneration system for Sinus cuspidatum based on callus induction

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[0015] The present invention is based on callus induction and establishes the method for high-frequency regeneration system of horsetail, comprising the following steps:

[0016] 1) explant screening: select the leaf that the healthy plant of Rhizoma japonica expands in spring, select the middle position of the leaf, and cut it off as an explant;

[0017] 2) Disinfection of explants: Put the leaves in step 1) in a 200mL beaker filled with clean water, add 2-3 drops of detergent, cover the mouth of the beaker with gauze, wash it with tap water, and add an appropriate amount of detergent Keep the flushing state at the tap water for 30-40 minutes, dry the water with filter paper; add 10% sodium hypochlorite solution with a mass percentage concentration in the beaker to completely soak the explant material, add 2 drops of Tween 20 (Tween20) to make disinfection After 15-20 minutes, pour out all the disinfectant in the beaker, pour the sterile water that has been sterilized by high...

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Abstract

The invention relates to a method for establishing a high-frequency regeneration system of Sinus chinensis based on callus induction. In the method, the leaves of S. radiata in spring are used as explants, MS is used as the basic medium, and kinetin (KT), 2, 4 Hormone regulation by chlorophenoxyacetic acid (2,4‑D), naphthaleneacetic acid (NAA), 6‑benzyladenine (6‑BA), indoxbutyric acid (IBA) to establish a method based on callus induction to establish Lin high frequency regenerative system method. In this invention, a multiplication and subculture cycle is 4-5 weeks, the callus induction rate is over 80%, and the callus adventitious bud induction coefficient is 8-10 times. The reproduction is not affected by the season, and the seedling reproduction coefficient can be greatly improved. , to maintain the original excellent traits, to provide a scientific and effective propagation method for the cultivation, application, and production of this variety, and to provide a reference for the factory production and propagation of Iris plants.

Description

technical field [0001] The invention relates to a method for establishing a high-frequency regeneration system of horsetails based on callus induction. Background technique [0002] The white-flowered horse rush (Iris.lactea Pall.) that the present invention relates to is a perennial herbaceous flower of the genus Iris of the family Iridaceae, with basal leaves, gray-green, strip-shaped or narrow sword-shaped; milky white flowers, 5-6 cm in diameter, and 4-7 cm long pedicel The flowering period is 5-6 months long; salt-alkali and trampling-resistant, the rhizome is thick and the root system is developed, which can be used for water and soil conservation, improvement of saline-alkali soil and fragile ecological environment; Landscaping landscape materials with high ornamental value. [0003] However, due to the cold and long winter in Northeast China and the short growing season in summer, there are few types of plants available for urban greening, and the cost of greening i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张兴曲彦婷王书可唐焕伟陈菲李黎韩辉熊燕石朔宇韩廷蔚陈颖张洪运李虹杨轶华孙波曲线
Owner HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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