Establishment of a high-frequency regeneration system for Sinus cuspidatum based on callus induction
A technology of callus induction and high-frequency regeneration, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of slow propagation speed of ramets, low multiplication coefficient, and inability to meet the needs of factory seedlings
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[0015] The present invention is based on callus induction and establishes the method for high-frequency regeneration system of horsetail, comprising the following steps:
[0016] 1) explant screening: select the leaf that the healthy plant of Rhizoma japonica expands in spring, select the middle position of the leaf, and cut it off as an explant;
[0017] 2) Disinfection of explants: Put the leaves in step 1) in a 200mL beaker filled with clean water, add 2-3 drops of detergent, cover the mouth of the beaker with gauze, wash it with tap water, and add an appropriate amount of detergent Keep the flushing state at the tap water for 30-40 minutes, dry the water with filter paper; add 10% sodium hypochlorite solution with a mass percentage concentration in the beaker to completely soak the explant material, add 2 drops of Tween 20 (Tween20) to make disinfection After 15-20 minutes, pour out all the disinfectant in the beaker, pour the sterile water that has been sterilized by high...
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