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Plant callus induction medium and preparation method of plant stem cells

A technology for inducing culture medium and callus, applied in the field of stem cells, which can solve the problems of reduced genetic stability of cell lines, somatic cell mutation, and slow growth cycle of plant stem cell culture.

Active Publication Date: 2020-11-13
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the existing plant stem cell culture growth cycle is slow, and the quantity cannot meet the production demand
Moreover, plant cell cultures undergo a dedifferentiation process in which somatic mutations occur, resulting in reduced genetic stability of the cell line

Method used

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  • Plant callus induction medium and preparation method of plant stem cells
  • Plant callus induction medium and preparation method of plant stem cells
  • Plant callus induction medium and preparation method of plant stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 obtains the callus tissue of tulip

[0052] Take the tulip bulbs of the Parade variety, peel off the outermost skin of the tulip bulbs, take the innermost scales and wash them with tap water for 10 minutes, 4-5 times with pure water, and put them into an ultra-clean workbench. Then wash twice with sterile water, soak in 75% alcohol for 30 seconds, wash twice with sterile water, wash with bromogeramine solution for 3 minutes, and wash with sterile water four times.

[0053] Cut the sterilized scales into 0.5-1.0cm with sterilized scissors 2 sized squares. MS complete medium (a medium for inducing callus growth) supplemented with exogenous stimuli was added, the specific formula is shown in Table 1, placed at 2°C for 5-6 weeks, and the growth of callus was observed every week. When a light yellow, irregular tumor-like callus grows, the callus induction is successful.

[0054] Table 1 MS complete medium (1L induces callus growth)

[0055] name c...

Embodiment 2

[0057] Embodiment 2 obtains the callus tissue of tulip

[0058] Take the tulip bulbs of the Parade variety, peel off the outermost skin of the tulip bulbs, take the innermost scales and wash them with tap water for 10 minutes, 4-5 times with pure water, and put them into an ultra-clean workbench. Then wash twice with sterile water, soak in 75% alcohol for 30 seconds, wash twice with sterile water, wash with bromogeramine solution for 3 minutes, and wash with sterile water four times.

[0059] Cut the sterilized scales into 0.5-1.0cm with sterilized scissors 2 sized squares. MS complete medium (medium for inducing callus growth) supplemented with exogenous stimuli was added, the specific formula is shown in Table 2, placed at 4°C for 5-6 weeks, and the growth of callus was observed every week. When a light yellow, irregular tumor-like callus grows, the callus induction is successful.

[0060] Table 2 MS complete medium (1L induces callus growth)

[0061] name con...

Embodiment 3

[0063] Embodiment 3 obtains the callus tissue of tulip

[0064] Take the tulip bulbs of the Parade variety, peel off the outermost skin of the tulip bulbs, take the innermost scales and wash them with tap water for 10 minutes, 4-5 times with pure water, and put them into an ultra-clean workbench. Then wash twice with sterile water, soak in 75% alcohol for 30 seconds, wash twice with sterile water, wash with bromogeramine solution for 3 minutes, and wash with sterile water four times.

[0065] Cut the sterilized scales into 0.5-1.0cm with sterilized scissors 2 sized squares. MS complete medium (medium for inducing callus growth) supplemented with exogenous stimuli was added, the specific formula is shown in Table 3, placed at 8°C for 5-6 weeks, and the growth of callus was observed every week. When a light yellow, irregular tumor-like callus grows, the callus induction is successful.

[0066] Table 3 MS complete medium (1L induces callus growth)

[0067] name con...

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Abstract

The invention relates to the technical field of stem cells and in particular relates to an induction culture medium of plant callus and a preparation method of plant stem cells. The induction culturemedium is prepared from the following components: 0.4 to 0.8mg / L of 2,4-D, 0.1 to 0.5mg / L of NAA (Naphthylacetic Acid), 1.5 to 2.5mg / L of 6-BA (6-Benzylaminopurine) and the balance of a basic culturemedium. According to the preparation method of the stem cells, provided by the invention, advantages of stem cell growth and hereditary characters are utilized, and the 2,4-D, the NAA and the 6-BA arecombined to induce explants, and cellulose hydrolase and pectin hydrolase are used for digesting the callus, and the stem cells with more quantity and good hereditary stability are separated within relatively short time; the method can keep the dryness of the stem cells of plants including tulips and the like and ensure the subsequent stability of cell growth genes, the method is not influenced by an external environment and lays a foundation for establishing a corresponding stem cell system.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a plant callus induction medium and a preparation method of plant stem cells. Background technique [0002] Medicinal plants refer to plants used in medicine for disease prevention and treatment. All or part of its plants are used for medicinal purposes or as raw materials for the pharmaceutical industry. In a broad sense, it can include plant resources used as nutrients, certain indulgences, condiments, color additives, and pesticides and veterinary medicines. There are many types of medicinal plants with different medicinal parts. For example, tulip is a perennial herbaceous plant belonging to the Liliaceae tulip family, which has the effect of eliminating dampness and warding off filth. It has been reported that the flowers and leaves of tulip contain a toxic alkaloid whose physiological effect is similar to Veratrine, and tuliposide ABC has an inhibitory effect on Bacil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 葛啸虎陈海佳王一飞吴子杰王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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