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Medium used for Cymbidium goeringii tissue culture

A technology of tissue culture and culture medium, applied in the fields of application, horticulture, botany equipment and methods, etc., can solve the problem of low survival rate of Chunlan and achieve the effect of improving the survival rate

Inactive Publication Date: 2016-12-21
张玉薇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a culture medium for Chunlan tissue culture, which can solve the problem of low survival rate of Chunlan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The protocorm culture medium of this embodiment uses 1 / 2 MS medium as the basic medium, and adds 0.3 mg / liter 2,4-dichlorophenoxyacetic acid, 10 g / liter palm oil, 0.2 mg / liter citric acid; pH value 5.7; the light is 2600LUX, and the temperature is 22℃~24℃;

[0016] Proliferation medium uses 2 / 3MS medium as the basic medium, adding 0.25 mg / L naphthaleneacetic acid, 0.2 mg / L gibberellin, and 0.3 mg / L nicotine; the light is 2550LUX, and the temperature is 22°C-26°C;

[0017] The medium for rooting and strong seedlings uses 2 / 3MS medium as the basic medium, adding 0.4 mg / L naphthaleneacetic acid and 0.5 mg / L cysteine; the light is 2650LUX, and the temperature is 22°C-26°C;

[0018] The specific culture method is as follows: take the shoot tip as the explant, first inoculate it in the protocorm medium to induce culture to produce the protocorm; then inoculate it in the proliferation medium to expand the culture, and finally transfer it to the rooting and strong seedling medi...

Embodiment 2

[0021] The protocorm culture medium of this embodiment uses 1 / 2MS medium as the basic medium, and adds 0.35 mg / liter 2,4-dichlorophenoxyacetic acid, 15 grams / liter palm oil, 0.25 mg / liter citric acid; pH value 5; the light is 2700LUX, and the temperature is 22℃~26℃;

[0022] Proliferation medium uses 2 / 3MS medium as the basic medium, adding 0.3 mg / L naphthaleneacetic acid, 0.15 mg / L gibberellin, and 0.2 mg / L nicotine; the light is 2500LUX, and the temperature is 22°C-25°C;

[0023] The medium for rooting and strong seedlings takes 2 / 3MS medium as the basic medium, adding 0.3 mg / L naphthalene acetic acid and 0.75 mg / L cysteine; the light is 2500LUX, and the temperature is 22℃~26℃

[0024] The specific culture method is as follows: take the shoot tip as the explant, first inoculate it in the protocorm medium to induce culture to produce the protocorm; then inoculate it in the proliferation medium to expand the culture, and finally transfer it to the rooting and strong seedling m...

Embodiment 3

[0027] The protocorm culture medium of this embodiment uses 1 / 2 MS medium as the basic medium, and adds 0.25 mg / liter 2,4-dichlorophenoxyacetic acid, 20 g / liter palm oil, 0.2 mg / liter citric acid; pH value 6; the light is 2500LUX, and the temperature is 22℃~26℃;

[0028] Proliferation medium uses 2 / 3MS medium as the basic medium, adding 0.3 mg / L naphthaleneacetic acid, 0.1 mg / L gibberellin, and 0.1 mg / L nicotine; the light is 2700LUX, and the temperature is 22°C-23°C;

[0029] The medium for rooting and strong seedlings takes 2 / 3MS medium as the basic medium, adding 0.5 mg / L naphthaleneacetic acid and 1 mg / L cysteine; the light is 2700LUX, and the temperature is 22℃~26℃;

[0030] The specific culture method is as follows: take the shoot tip as the explant, first inoculate it in the protocorm medium to induce culture to produce the protocorm; then inoculate it in the proliferation medium to expand the culture, and finally transfer it to the rooting and strong seedling medium fo...

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Abstract

The invention discloses a medium used for Cymbidium goeringii tissue culture, and relates to the technical field of flower tissue culture. 2,4-Dichlorophenoxyacetic acid, palm oil, citric acid and other substances are added to a basal medium to make the medium meet nutrients, the pH value and other parameters needed by protocorm induction in order to make the success rate of protocorm formation reach 88.5%, and nutrient components and the ratio of the above obtained enrichment medium and a rooting and seedling strengthening medium are adjusted in order to improve the survival rate of Cymbidium goeringii and make the survival rate reach 91% or above.

Description

technical field [0001] The invention relates to the technical field of flower tissue culture, in particular to a culture medium for Chunlan tissue culture. Background technique [0002] Chunlan, whose scientific name is Cymbidium goeringii, is a kind of orchid of the genus Cymbidium in the subfamily Cymbidium, belonging to the ground orchid, Orchidaceae. Perennial evergreen herb. Chunlan is used to propagate ramets, combined with changing pots, and can be sown or tissue cultured where conditions permit. It likes warm, humid, semi-shady, loose, well-drained fertile soil, not cold-resistant, avoid high temperature, dryness, and direct sunlight. It is one of the traditional famous flowers in China. Chunlan has the functions of clearing the lungs and removing heat, resolving phlegm and relieving cough, cooling blood and stopping bleeding; and as an indoor ornamental flower, it has a special and elegant aroma. There are flowers all year round. It is a good product for indoor d...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张玉薇
Owner 张玉薇
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