Method for cultivating cymbidium goeringii seed by tissue culture

Abstract

The invention relates to a method of seedling with tissue culturing by using spring orchid seed, comprising the following steps: taking the capsule of not broke up from spring orchid as explant; immersing with alcohol or wiping it with absorbent cotton dipped with alcohol, cutting up the capsule to fetch out the pulverulent seed, loading it into sheeting bag, immersing it in the alcohol, putting it in the NaOH solution with concentration being 0.08-0.12mol / l for 8-10 minutes, putting it into sodium hypochlorite solution of 10.5í‚2% for 6-8 minutes; sowing the treated seeds on the culturing medium for seed germination, lighting 14-16 hours fir every day, with the luminous intensity being 1000-1500lux, temperature 23í‚2 Deg C, culturing for 160-180 days, and growing out original bulb; transferring the original bulb to the culturing medium for seed germination the same with mentioned above for successive transfer culture, for 50-60 days, and growing out new sprout or original bulb, cutting the original bulb continuously for successive transfer culture and reaching the geometric extension. The invention employs method of tissue culturing by using spring orchid seed, to breed species of high quality and to choose orchid plant from seed seedling.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for raising seedlings of Chunlan by adding different components to a basic medium. Background technique: [0002] Chunlan is a kind of ground-growing orchid. It is native to China and has a long history of cultivation. It not only has beautiful flowers and elegant fragrance, it is known as "a gentleman among flowers"; In addition, it also has high medicinal value and can treat various diseases. With the growth of the world economy, the demand for rare and precious varieties of orchids in China, especially Chunlan, is also increasing. [0003] The germination of Cymbidium plant seeds has accumulated a lot of materials, but the seeds of some ground orchids in the Cymbidium plants are not easy to germinate artificially, and such seeds are generally called difficult-to-germinate seeds. Although there are many varieties of Chunlan, they are all selected from th...

AI Technical Summary

Problems solved by technology

Due to the failure to solve the problem of mass reproduction, farmers went to the mountains to dig a large number of wild Chunlan, and the natural resources were seriously damaged. It is difficult to find wild Chunlan plants in areas with relatively convenient transportation.

[0004] Since ancient times, the fine varieties of Chunlan have only been selected from natural variation, which is time-consuming, inefficient, and slow to improve varieties. With the development of horticulture today, modern breeding methods have almost changed the appearance of all flowers, but they have not been widely used in Chunlan.