1882 results about "Senescence function" patented technology

The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.

The present invention relates to compounds of formula I:and pharmaceutically acceptable salts, stereoisomers or tautomers thereof which are inhibitors of poly (ADP-ribose) polymerase (PARP) and thus useful for the treatment of cancer, inflammatory diseases, reperfusion injuries, ischemic conditions, stroke, renal failure, cardiovascular diseases, vascular diseases other than cardiovascular diseases, diabetes, neurodegenerative diseases, retroviral infection, retinal damage or skin senescence and UV-induced skin damage, and as chemo- and / or radiosensitizers for cancer treatment.

Methods are provided herein for selectively killing senescent cells and for treating senescence-associated diseases and disorders by administering a senolytic agent. Senescence-associated diseases and disorders treatable by the methods using the senolytic agents described herein include cardiovascular diseases and disorders associated with or caused by arteriosclerosis, such as atherosclerosis; idiopathic pulmonary fibrosis; chronic obstructive pulmonary disease; osteoarthritis; senescence-associated ophthalmic diseases and disorders; and senescence-associated dermatological diseases and disorders.

Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced eIF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced eIF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.

Methods of producing a protein in cell culture comprising an anti-senescence compound, such as the antioxidant carnosine, are provided. According to teachings of the present invention, cells grown in a cell culture medium comprising an anti-senescence compound exhibit increased viability and productivity. Furthermore, cell cultures grown in the presence of an anti-senescence compound exhibit decreased levels of high molecular weight aggregates in the cell culture medium.

An automated relative maturity system for measuring the relative maturity of a large number of plots of diverse varieties of plants growing in a field or fields. A field to be evaluated is laid out in multiple plots with a specific variety assigned to a preselected plot or plots and with areas set aside throughout the field for planting of check varieties of known relative maturity. High-precision GPS is used with a planter to record the location of each plot within the field on a map. When leaf senescence is under way throughout the field, a radiometric crop sensor mounted on a vehicle also equipped with high-precision GPS is used to scan the plants in the plots to record readings of the plants synchronized to the GPS map locations, including the check plants of known relative maturity. Software is used to calculate the relative maturity of each variety.

Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced elF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced elF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.

Methods of producing a protein in cell culture comprising an anti-senescence compound, such as the antioxidant carnosine, are provided. According to teachings of the present invention, cells grown in a cell culture medium comprising an anti-senescence compound exhibit increased viability and productivity. Furthermore, cell cultures grown in the presence of an anti-senescence compound exhibit decreased levels of high molecular weight aggregates in the cell culture medium.

Fibrosis arises as part of a wound healing response that maintains organ integrity following catastrophic tissue damage, but can also contribute to a variety of human pathologies, including liver cirrhosis. The invention demonstrates that cellular senescence acts to limit the fibrogenic response to tissue damage, thereby establishing a role for the senescence program in pathophysiological settings beyond cancer. Accordingly, the methods of the invention relate to modulating cellular senescence in disease tissue that have elevated numbers of senescent cells, such as in fibrotic tissues.

A method of prevention / treatment of pathological consequences of DNA damage triggered by incorporation of circulating apoptotic chromatin fragments into healthy cells of individuals / patients in need therefore, said method comprising ex vivo or extra corporeal treatment of blood / plasma for removal of circulating chromatin fragments released from apoptotic cells which apoptotic chromatin fragments are capable of triggering DNA damage leading to genomic instability, senescence, apoptosis and cancerous transformation of healthy cells on being integrated into their genomes

The invention relates to a production process of germ and corn composite nutritional powder and the product of the production process, and belongs to the technical field of food processing. The production process is characterized in that mixing corn which sprouts in stress with wheat germ which is subjected to microwave enzyme inactivation; performing extrusion and superfine grinding to prepare germ and corn meal; adding carrot meal, spirulina meal, white granulated sugar, maltodextrin and vegetable fat powder into the germ and corn meal; and sterilizing and packaging to prepare the germ and corn composite nutritional powder. The production process is simple and high in industrial degree, so that the germ resource can be further utilized, corn can be completely utilized, and the additional value of agricultural products can be increased. The germ and corn composite nutritional powder produced by the production process is good in dissolubility, fine in taste and high in nutritional value, is rich in gamma-aminobutyric acid, glutathione, octacosanol, dietary fiber and other components, has the functions of improving brain functions, calming nerves, improving sleeping, resisting oxidation, delaying senescence, improving body immunity and the like, and is an ideal health-care nutritional food, wherein the content of gamma-aminobutyric acid in the product is between 20 and 60mg / 100g.

Polynucleotides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The encoded polypeptides of the invention have been shown to confer at least one regulatory activity and confer greater size, greater organ size, greater biomass, greater yield, curlier leaves, darker coloration, greater tolerance to water deprivation, delayed flowering, delayed development, delayed senescence, greater tolerance to cold, and / or greater tolerance to hyperosmotic stress as compared to a control plant.

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

Provided herein are methods and uses for treatment or prophylaxis of a senescent cell associated disease or disorder by administering a senolytic combination comprising dasatinib and quercetin or an analog thereof to a subject in need thereof. In certain embodiments, the senescent cell associated disease or disorder is a cardiovascular disease or disorder, inflammatory disease or disorder, a pulmonary disease or disorder, a neurological disease or disorder, or a metabolic disease or disorder.

The present invention relates to a novel method for expanding mesenchymal stem cells (MSCs) in low-density and hypoxic condition as compared to normal air conditions traditionally used in cell culture. The present method provides rapid and efficient expansion of human MSCs without losing cellular proliferation and stem cell properties, including increase in proliferation, decrease in senescence, and increase in differentiation potential both in vitro and in vivo. The expanded MSCs by the present method may maintain normal karyotyping, and will not form tumor when transplanted into mamma.

The invention discloses an active composition containing copper peptide and a cosmetic or skin care product. The active composition containing copper peptide is prepared from the following components in percentage by weight: 0.001-2 percent of copper peptide, 1-5 percent of vitamin E, 0.1-10 percent of chitin oligosaccharide, 0.1-2 percent of 4-MSK, 0.1-5 percent of algal polysaccharides, 6-30 percent of emulsifier, 0-10 percent of co-emulsifier, 0.1-0.8 percent of stabilizer, 0-10 percent of excipient and the balance of water. The active composition and the cosmetic or skin care product have the effects of promoting cell reproduction, remarkably moisturizing and moistening, delaying senescence and the like.

A problem to be solved of the present invention is to provide a drug formulation and a method of administering an active ingredient which allow the active ingredient to be delivered evenly into the site of action in the skin with high efficiency while ensuring stability of the active ingredient over a long time, and which are easily handled and are less stressful for patients. Mean for solving the problem is a microneedle assembly formulation for skin treatment comprising a platform and a plurality of conical or pyramidal microneedles formed on the platform containing a base composed of a bio-soluble and thread-forming polymer substance and an objective substance retained in the base, wherein the objective substance is a substance effective for prevention or treatment of skin senescence, or treatment of skin scar.

The invention belongs to the technical field of daily care products and discloses a facial mask with the functions of improving skin barrier and delaying senescence and a preparation method thereof. The facial mask comprises the following components in percentage by weight: 0.05-4 percent of macromolecule hyaluronic acid, 0.1-3 percent of middle molecule hyaluronic acid, 0.1-5 percent of micromolecule hyaluronic acid, 0.05-3 percent of oligomeric hyaluronic acid, 0.5-6 percent of grape seed extract, 0.5-6 percent of purslane extract, 2-12 percent of hydroxyethyl urea, 1-6 percent of sodium pyrrolidonecarboxylate, 0.1-1 percent of allantoin, 2-12 percent of butanediol, 0.05-0.3 percent of thickening agent and the balance of deionized water. According to the facial mask disclosed by the invention, by reasonably combining natural compounds with plant extracts, the synergistic effect is realized, and the functions of better improving the skin barrier and delaying the senescence are achieved.

The invention particularly discloses lucid ganoderma tea and a preparation method of the lucid ganoderma tea. The lucid ganoderma tea is prepared from the following raw materials in parts by weight: 20-30 parts of lucid ganoderma, 10-20 parts of broken spore ganoderma lucidum powder, 15-20 parts of mushrooms, 15-20 parts of dry cargoes, 10-15 parts of pollens and 10-15 parts of tea leaves. The lucid ganoderma tea disclosed by the invention has the beneficial effects that the lucid ganoderma tea is edible and officinal, can improve the immune function, balance nutrition in vivo and accelerate metabolism, and lucid ganoderma and tea leaves are effectively combined, so that not only is the health-care function of lucid ganoderma reserved, but also the lucid ganoderma tea has faint scent of tea leaves and solves the problem of bad taste; the lucid ganoderma tea contains various health-care auxiliary materials and edible and officinal materials which assist each other, so that not only is the effect of lucid ganoderma improved, but also other health-care functions are increased; the functions of the five internal organs are adjusted by nourishment, so that the lucid ganoderma tea has the effects of improving sleep, improving immunity, delaying senescence and comprehensively conditioning human body; different raw materials can be matched and combined to fit selections of different people and tastes.

The invention discloses a dendrobium officinale stem cell and an isolated culture method thereof. The method comprises the following steps: culturing plant root tissue containing a quiescent centre, performing subcultring and scale-up culture on quiescent centre stem cell, and then preparing stem cell dry powder or extracting solution according to requirements. The dendrobium officinale stem cell overcomes the problem that dendrobium officinale in vitro material is easy to brown, the cell of a stem cell line is not differentiated, the cell increment is large in a nutritional condition, the culture time is short, and the cell activity is high. The dendrobium officinale stem cell and the method have the advantages that the technical process is easy to operate, the quantity of technological processes is less, the output is high, and the dendrobium officinale stem cell obtained is anti-aging and oxidation resistant, promotes hair growth and high baldness-resistant activity, and can be manufactured into functional health care products and cosmetics for preventing and curing various senescence and baldness and relevant Chinese drugs for preventing senescence and baldness.

The invention discloses a skin moisturizer containing rose ferment and a preparation method thereof. The skin moisturizer comprises rose ferment, vinegar, glycerol and propylene glycol, and is prepared by the following steps: fresh rose is cleaned, smashed and pulped, a slurry is obtained, and then fruits and vegetables and Chinese herbal medicine are mixed; then water and cellulase powder are added for reacting and resolving, and through yeast fermentation, filtering and slag removing, rose ferment is obtained; then the vinegar, the glycerol and the propylene glycol are added into the rose ferment, uniform stirring and sealing are performed, and still standing is performed for 7 days at the temperature of 25 to 30 DEG C, so as to obtain the skin moisturizer. The skin moisturizer containing rose ferment provided by the invention has the advantages of being free of any preservative components, being inirritative to skin, and playing effects of whitening and delaying senescence, and has a wide market development prospect.

The invention relates to a polysaccharide buccal tablet prepared from Camellia nitidissima, which mainly comprises the following raw materials: polysaccharide substances extracted from the Camellia nitidissima, a sweetening agent, trehalose, citric acid and the like. The preparation method comprises the following steps: adopting supersonic waves to leach the Camellia nitidissima first, adopting a nano-filtration method to perform concentration production of a concentrated Camellia nitidissima solution, performing spray drying, tabletting and package, and obtaining the product. The product has the functions of reducing blood sugar, blood pressure, blood fat and cholesterol in serum, inhibiting tumor growth, preventing atherosclerosis, activating various enzymes in human body, improving the immunological competence of the body, prolonging senescence, improving digestion of intestinal canals, and the like, has complete health-care functions, is suitable to be eaten by common people, and has obvious health-care effect.

The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and / or augment the functionality of such cells for cellular therapies.

The invention relates to wine preserving health and keeping young. The production method and characteristic mixture ratio of the invention are as follows: medlar, shizandra berry sesame, milk veteh, processed fuzi, walnut meat, prepared polygalae, achyranthes, prepared fleece flower root, cibotium root, dodder, leek seed, epimedium powder, root of rehmannia, cinnamon, cynamorlum herba cistanches, fructus cnidii selinum, tuckahoe, cedar seed, raspberry, ligustrum lacidum aiton, caulis cynomorii, prepared rhizome of rehmannia, angelica, fructus psoraleae, 230 to 260g of honey, and 300 to 9000g of high-quality daqu wine of 55 degrees added to be heated to boil and form the invention after cooling and filtering, followed by bottling. The wine has the effects of invigorating vital energy, hematogenesis, invigorating blood circulation, tranquilizing by nourishing the heart, benefiting kidney, yin and yang, strengthening muscle and bone, through channels, warming the kidney and strengthening yang, benefiting sperm and supplementing marrow, protecting liver and strengthening spleen, attempering five Zang-organs and six Fu-organs, growing and blacking hair, maintaining beauty and revivifying, flushing the spirit, eliminating fatigue, promoting metabolism, activating senile cell, building up immune function of human, delaying senescence and keeping youth.

The invention discloses a ginseng-medlar solid beverage and in particular relates to a ginseng-medlar solid beverage taking Chinese herbal medicines comprising ginseng and medlar as main raw materials. The invention aims at solving the technical problems of time waste caused by long-time decoction in a current common Chinese herbal medicine taking manner and narrow applicable ranges of tablets, particles, capsules which are produced by extraction process by using traditional Chinese medicine extracts as main components. The ginseng-medlar solid beverage is prepared by the following raw materials in parts by weight: 200-300 parts of ginseng, 1,400-1,600 parts of medlar, 500-600 parts of jujube, 800-900 parts of raspberry, 800-900 parts of mulberry and 900-1,100 parts of sealwort. According to the technical scheme of the ginseng-medlar solid beverage, modern processes such as vacuum concentration and centrifugal separation are adopted; the quality of product is comprehensively improved by improving nutrition, taste and absorption; the ginseng-medlar solid beverage has the effects of improving qi and blood, building fitness, resisting fatigue, enhancing immunity and delaying senescence.

The present invention relates to compounds of formula I:and pharmaceutically acceptable salts, stereoisomers or tautomers thereof which are inhibitors of poly (ADP-ribose) polymerase (PARP) and thus useful for the treatment of cancer, inflammatory diseases, reperfusion injuries, ischemic conditions, stroke, renal failure, cardiovascular diseases, vascular diseases other than cardiovascular diseases, diabetes, neurodegenerative diseases, retroviral infection, retinal damage or skin senescence and UV-induced skin damage, and as chemo- and / or radiosensitizers for cancer treatment.

The invention provides a processing method of mushroom fragrance beef. The processing method of the mushroom fragrance beef comprises the following working procedures of marinating beef, processing mushrooms, blending, packaging in vacuum, sterilizing, putting into storage and the like. The mushroom fragrance beef prepared by the processing technology keeps nutritional ingredients of beef and mushroom-type substances maximally, is high in protein, low in fat, and rich in nutrition, has mellow taste, also has the efficacies of softening blood vessels, moistening skin, delaying senescence and the like, can lower fat and sugar if being eaten frequently, has the efficacy of improving immunity, has a scientific processing technology, avoids the generation of nitrite, and is healthy in eating.

The invention discloses a composition for preparing a camel milk facial mask, and particularly a composition for preparing a camel milk moisturizing skin-calming facial mask, a camel milk moisturizing clear facial mask, a camel milk moisturizing compact facial mask and a camel milk moisturizing conditioning facial mask according to functions. By virtue of the synergism of camel milk, water, sodium carbomer, glycerinum, xanthan gum, butanediol, sodium hyaluronate, poly(sodium L-glutamate), skin conditioner and preservative, the characteristics and advantages of the camel milk are maximally maintained, so that the composition for preparing the cameral milk facial mask has the advantages of the camel milk, namely, proteins, vitamins, minerals and fatty acid rich in the composition can provide rich nutrient substances for the skin and can improve the face skin; particularly, the camel milk contains lactoferrin and lysozyme which are short in cow milk, so that the composition has the effects of sterilizing, killing demodex, removing toxicity, softening stratum corneum epidermidis, moisturizing and tendering the skin, preventing wrinkles and aging resistance, and delaying senescence.

The invention belongs to the field of food processing, and particularly relates to greengage sweetmeat containing black tea and a production method of the greengage sweetmeat. The greengage sweetmeat containing the black tea comprises the following raw materials in parts by weight: 100 parts of fresh greengage fruit, 30-50 parts of white granulated sugar, 8-10 parts of salt, 1-5 parts of black tea, and 0.5 parts of alum. The production method comprises the steps of selecting materials, drilling, washing, salt curing, drying, dipping for desalting, sugaring, black tea flavoring, draining, stoving, and packaging in sequence. In the process procedure of the greengage sweetmeat, tea soup is adopted for the flavoring; on the one hand, a color of the tea is adopted to allow the greengage sweetmeat to be redder and glossy; the taste of the black tea allows the flavor of the greengage sweetmeat to be richer; and the mouthfeel is sweeter; on the other hand, due to the combination of nutrition and health care functions of both the greengage and the black tea, functions of the greengage and the black tea on human bodies are made full use of and effects such as nutrition, health care, cancer prevention, blood glucose reducing, fatigue removing, and senescence delaying are reached.