139results about How to "Cause death" patented technology

Aspects of the invention provide methods for harnessing the potential of proteins that occur naturally (e.g., in humans) and that have serious but finite toxicity. Aspects of the invention relate to a quantitative systems-biological and structural approach to design a class Mof chimeric proteins that avoid the toxicity of protein drugs while retaining their desired activities. In particular, chimeric proteins containing a variant form of a natural protein fused to a targeting moiety may be administered to a subject to target a signal (e.g., induction of apoptosis) to particular cells without having a generalized toxic effect

The invention discloses an integrated type ecological floating bed and the water body ecological system repairing technology thereof. A physical space of the integrated type ecological floating bed is formed by constructing a floating bed, a sinking bed, a buoyancy adjusting bottle, a lifting chain and an ecological barricading net, and integrates ecological components such as indigenous emergent aquatic plants, submerged plants, attached aquatic plants, fishes, snails and biochemical zeolite. The ecological behaviors of the ecological components are in linear cause and effect relation. The ecological components and organisms in the physical space form a semi-enclosed ecological subsystem with integral completeness. The upper line and the water body ecological system to be repaired are strong in coupling capability, and ecological factors in the lower line can exist stably. The damaged water body ecological system sufferring from one or a plurality of types of pollutions including water body eutrophication, heavy metal pollution, organic pollution and the like can be repaired by building pioneer communities and utilizing self-cleaning capability of the ecological system, pollution is removed, integral restoration or rebuilding of the damaged water body ecological system is promoted, simultaneously environment is beautified, and ecological deterioration of a healthy water body can be prevented.

Provided herein are methods and uses for treatment or prophylaxis of a senescent cell associated disease or disorder by administering a senolytic combination comprising dasatinib and quercetin or an analog thereof to a subject in need thereof. In certain embodiments, the senescent cell associated disease or disorder is a cardiovascular disease or disorder, inflammatory disease or disorder, a pulmonary disease or disorder, a neurological disease or disorder, or a metabolic disease or disorder.

It is disclosed here that HMG-CoA reductase inhibitors inhibit the proliferation and cause the death of breast cancer cells by inducing the expression of inducible nitric oxide synthase (iNOS) to promote intracellular nitric oxide formation, which the inventors found to be accomplished through the inhibition of protein geranylgeranylation. The disclosure here enables a new breast cancer treatment strategy that combines the inhibition HMG-CoA reductase or protein geranylgeranylation and the promotion of nitric oxide formation by iNOS.

Provided is a method for the functionalization of immunoglobulins through the use of transglutaminase, including methods for screening functionalized antibodies for characteristics of interest, antibody compositions comprising a plurality of functionalized antibodies, and functionalized antibodies with rodent constant regions.

The invention discloses a modeling method of an Sjogren syndrome mouse model. The modeling method comprises the following steps: killing mice, taking out bilateral salivary glands and peeling off capsules and connective tissues; washing with PBS (Poly Butylenes Succinate); adding the PBS according to the amount of adding 0.5ml of the PBS into each salivary gland; shearing the salivary glands into pieces, and uniformly homogenizing and centrifuging in an ice bath; then taking supernatant and quantifying salivary gland antigens by adopting a BCA (Bicinchoninic Acid) protein quantifying method; adjusting the concentration of the salivary gland antigens to be 4mg/ml by utilizing the PBS; adding equal quantity of an FCA (Freund Complete Adjuvant) or an FIA (Freund Incomplete Adjuvant) and diluting the concentration to be 2mg/ml; repeatedly blowing and beating until two liquid phases are dissolved mutually to form an ivory color; randomly grouping C57BL/6 mice and shaving off furs on the backs of the mice; carrying out intradermal multi-site injection of 2mg/ml mouse salivary gland antigens prepared by the FCA on the back and tails of the mice on the current day and the 7th day, wherein the injection amount is 0.1ml per mouse; injecting equal quantity of the salivary gland antigens prepared by the FIA on the 14th day through the same method; after modeling for about 6 weeks, detecting indexes and screening the successfully modeled mice. The modeling method disclosed by the invention is high in modeling efficiency and short in modeling time.

The invention relates to a highway green belt and a construction method thereof and belongs to the technical field of civil engineering. The highway green belt comprises a slope green belt and an isolating green belt, wherein the slope green belt comprises a base body, a reservoir, a drainage ditch and a planting pit, the isolating green belt comprises a fence, a soil layer, a water storage layer and a base layer, and the reservoir is connected with the water storage layer through connecting pipelines. The construction method comprises steps as follows: base body construction of the slope green belt, construction of the isolating green belt and cultivation of green plants. According to the highway green belt and the construction method, the cost is low, the maintenance frequency and maintenance cost are reduced, construction is simple, death of trees is reduced to the maximum, and management and maintenance of the green belt are facilitated.

The invention belongs to the technical field of seedling cultivation, and particularly relates to a full-automatic seedling cultivation device. The full-automatic seedling cultivation device comprisesa cultivation box, a first feeding opening, a second feeding opening, a supporting seat and a controller; the first feeding opening and the second feeding opening are formed in the top of the cultivation box; a rotary cylinder is arranged at the bottom of the cultivation box; a piston end of the rotary cylinder penetrates through the cultivation box and extends into the cultivation box; a pistonend of the rotary cylinder is in threaded transmission with an input end; a rotating unit is arranged above the piston end of the rotary cylinder; a first stirring unit is arranged in the upper portion of the cultivation box; and a second stirring unit is arranged below the first stirring unit. The rotating unit, the first stirring unit and the second stirring unit cooperate, the rotary cylinder provides power, full-automatic seedling cultivation is realized, and the seedling cultivation quality is ensured while the labor amount is reduced.

The invention discloses application of a 5-bit modified 2'deoxycytidine analogue or phosphate thereof in the preparation of medicaments for treating cancers. The 5-bit modified 2' deoxycytidine analogue or the phosphate thereof can also be used in combination with deoxythymidine or phosphate thereof to form a medicinal composition for treating tumors. A 5-bit modified 2' deoxycytidine derivative is added with an appropriate amount of thymidine or 5' monophosphate when administrated, so that the toxicity to normal cells is greatly reduced to keep the effect of inhibiting tumor cell proliferation.

The invention relates to a preparation method of a microporous polyethylene mildew-proof preservative film, and belongs to the technical field of environment-friendly materials. A chemical reduction method is applied to prepare a chitosan nano-silver solution, taking the sodium borohydride as a reducing agent and chitosan as a stabilizing agent. A large amount of hydroxyl groups and amino groups on molecular chains of the chitosan can react with nano-silver to stabilize the nano-silver solution. Nano-TiO2 and nano-SiO2 are added into the chitosan nano-silver solution to prepare a composite nanomaterial. Through contact reaction of the nano-silver, water resistance of the nano-SiO2 and photocatalytic reaction of the Nano-TiO2, inherent ingredient respiratory systems and material transfer systems of pathogenic microorganisms are damaged, reproduction of the pathogenic microorganisms are disabled to promote cell death, and accordingly, bacteria resistance, freshness retaining and moistureresistance are realized. The prepared microporous polyethylene mildew-proof preservative film has high moisture-penetrability, high barrier property, and excellent application and market prospect.

The invention discloses a fish fry grading selection pond. The fish fry grading selection pond is composed of a fry culturing storage pond, a strip-shaped selection pond and a fry culturing pond, wherein the fry culturing storage pond and the fry culturing pond are respectively provided with an independent filtering circulating system, a water inlet pipe, a water discharge pipe, a poroid structure and a bottom opening, each bottom opening is provided with a net piece, a fish sucking pipe is arranged on the side wall of the fry culturing storage pond, the fish sucking pipe is provided with a fish sucking pump and a fish control valve, the fry culturing storage pond temporarily raises fries to be selected, the fry culturing pond is provided with a fish conveying pipe and a water control pipe, a net piece is covered at the top of the water control pipe, and the fry culturing pond is used for culturing the selected high-quality fries. The strip-shaped selection pond is supported by supporting pillars and is used for operation of fry selection. The upper portions of the fry culturing storage pond, the strip-shaped selection pond and the fry culturing pond are connected through poroid structures, and the bottoms of the fry culturing storage pond, the strip-shaped selection pond and the fry culturing pond are communicated through an underground pipeline. The fish fry grading selection pond is simple in structure, and brings convenience to fry grading selection. Due to the fact that controllable performance is high in the entire operation process, the fries do not die easily caused by oxygen deficit and mechanical injury. A fish control valve can effectively adjust the density of the fries in the strip-shaped selection pond, and the fry selection efficiency is improved.

The invention provides a compound preparation for killing wild fish and snail and a preparation method thereof; the compound preparation for killing the wild fish and the snail comprises the following components by weight parts: 40 to 50 parts of tea saponin, 60 to 80 parts of areca, 40 to 60 parts of niclosamide and 30 to 40 parts of zeolite powder; the preparation method uniformly stirs the raw material tea saponin, areca, niclosamide and zeolite powder by weight parts; the compound preparation for killing wild fish and snail promotes the dysfunction and the death of all organs and systems of the wild fish and the snail through the cooperation of the tea saponin, the areca and the niclosamide, has the advantages of strong killing effect and safety to shrimps and crabs, and effectively prevents the situations that the wild fish, the snail and the like fight over bait, nutrition, space and the like with the shrimps, the crabs and the like; and the preparation method is simple, and is applicable to the needs of scale industrial production.

The invention relates to a kind of compound medicine made of vitro cultured ox gallstone and nitroimidazole medicine, the ratio of the two kinds of medicine is 1:1-1000. The invention has combined the traditional Chinese medicine theory and modern pharmacodynamics theory and chosen nitroimidazole antibiotics (with great antibacterial activity) and vitro cultured ox gallstone (with obvious effects of anti-inflammatory and relieving fever) as the main material medicine. The combining effects of the medicine is very good with the advantage of quick to relieve pain and eliminate inflammation, radical cure, difficult to recur and low side effects.

The invention discloses a kaffir lily cultivation medium. The kaffir lily cultivation medium comprises raw materials in parts by weight as follows: 10-20 parts of construction waste particles, 5-12 parts of fish scale, 8-15 parts of fish bone meal, 10-17 parts of pine needles, 5-10 parts of distillers' grains, 5-20 parts of plant ash, 10-30 parts of animal waste, 3-8 parts of glutinous rice, 1-2 parts of a tourmaline powder anion additive, 12-28 parts of attapulgite clay, 2-4 parts of monopotassium phosphate, 0.5-0.8 parts of aspartic acid, 3-5 parts of urea, 0.2-0.6 parts of glycine, 10-20 parts of perlite, 0.1-0.3 parts of indoleacetic acid, 1-2 parts of boric acid, 0.3-0.6 parts of gibberellin, 2-3 parts of a microbial fermentation agent and 1-2 parts of activated carbon. According to the kaffir lily cultivation medium, the defect that an existing cultivation medium for planting kaffir lily has too high requirements for temperature, humidity, pots, daily care and the like is overcome, and survival rate and flowering rate are increased.

The invention provides a separation and extraction fluid suitable for pig pancreas islet and a preparation thereof. The fluid is characterized in that the oxygen load of the separation and extraction fluid is high and the problem of oxygen deficit in the separation and extraction process of pancreas islet can be solved. The separation and extraction fluid comprises the following steps of: first, taking a basal culture medium or a balanced salt solution as an aqueous phase; and adding collagenase, calcium chloride, DNA enzyme, bovine serum albumin, perfluorocarbon, an emulgator and an antibacterial agent for meeting the demand of separating and extracting pig pancreas islet. The separation and extraction fluid provided by the invention cannot release the anoxic degree in the separation and extraction process of pancreas islet and the survival rate, the function and the activity of the pancreas islet treated by the separation and extraction fluid provided by the invention are superior to those of pancreas islet treated by the conventional separation fluid with collagenase only.

The invention belongs to the technical field of kidney injury treatment drugs, and particularly relates to application of ponatinib as a drug for acute kidney injury. As an acute clinical complication, the acute kidney injury has the characteristics of being high in morbidity and high in mortality, and effective treatment drugs are not achieved at present. Related researchers in the field show that PDCD4 expression becomes higher in an acute kidney injury model, and it is indicated that the PDCD4 plays an important role in the physiological mechanism related to the acute kidney injury. The situation that the PDCD4 aggravates the kidney injury by regulating an Fgr-Stat3-Notch1 pathway is further confirmed. The confirmation of the regulatory mechanism provides a basis for the treatment of the acute kidney injury. According to the research results, the ponatinib, an Fgr kinase inhibitor, is adopted to treat the acute kidney injury, the drug has a significant protective effect on the injury caused by kidney ischemia reperfusion, and it is indicated that the drug intervention in Fgr can prevent and treat the acute kidney injury.