Modeling method of Sjogren syndrome mouse model

Abstract

The invention discloses a modeling method of an Sjogren syndrome mouse model. The modeling method comprises the following steps: killing mice, taking out bilateral salivary glands and peeling off capsules and connective tissues; washing with PBS (Poly Butylenes Succinate); adding the PBS according to the amount of adding 0.5ml of the PBS into each salivary gland; shearing the salivary glands into pieces, and uniformly homogenizing and centrifuging in an ice bath; then taking supernatant and quantifying salivary gland antigens by adopting a BCA (Bicinchoninic Acid) protein quantifying method; adjusting the concentration of the salivary gland antigens to be 4mg / ml by utilizing the PBS; adding equal quantity of an FCA (Freund Complete Adjuvant) or an FIA (Freund Incomplete Adjuvant) and diluting the concentration to be 2mg / ml; repeatedly blowing and beating until two liquid phases are dissolved mutually to form an ivory color; randomly grouping C57BL / 6 mice and shaving off furs on the backs of the mice; carrying out intradermal multi-site injection of 2mg / ml mouse salivary gland antigens prepared by the FCA on the back and tails of the mice on the current day and the 7th day, wherein the injection amount is 0.1ml per mouse; injecting equal quantity of the salivary gland antigens prepared by the FIA on the 14th day through the same method; after modeling for about 6 weeks, detecting indexes and screening the successfully modeled mice. The modeling method disclosed by the invention is high in modeling efficiency and short in modeling time.

Description

technical field [0001] The invention relates to the field of medical experiments, in particular to a method for making a mouse model of Sjogren's syndrome. Background technique [0002] Sjögren syndrome (SS) is a chronic inflammatory autoimmune disease characterized by invasion of salivary and lacrimal glands. Sjögren's syndrome can be divided into primary and secondary according to the cause of the disease. Primary Sjögren's syndrome (primary Sjogren Syndrome, pSS) is a global disease that occurs more frequently in women and the elderly, with a male to female ratio of about 1:9 to 1:20. Dry mouth and dry eyes are the typical clinical symptoms of the disease, and some patients also have intermittent parotitis and later rampant dental caries; in addition, it includes other atypical extraglandular system symptoms. [0003] At present, the main animal models of Sjögren's syndrome at home and abroad are mice, including two types, namely spontaneous and induced. Spontaneous an...

AI Technical Summary

Problems solved by technology

Although many organ damages in spontaneous animal models are quite similar to those of Sjögren's syndrome in humans, the high cost, long cycle, difficult control and low modeling rate make laboratory research encounter many difficulties

At present, there are deficiencies in several induced animal models at home and abroad. For example, the use of rats to create models requires high economic costs, and the long cycle will cause the functions of various tissues and organs of rats to gradually decline, thereby affecting the accuracy of the experiment. sex

In addition, the route, dose, time, frequency and selection of adjuvant induced by mouse antigen will all affect the success rate of the model.

Images