The invention discloses an egg
yolk antibody and a preparation method and device thereof. A
test object is used for performing
polypide reinvigoration and proliferation on tender eimeriida, giant eimeriida and poisoned eimeriida, after the insects are mixed in equal proportions,
antigen is prepared by ultrasonic disruption, the obtained
antigen solution is mixed with an equal volume of freund's
complete adjuvant and freund's incomplete
adjuvant separately, laying hens are immunized three times, egg
yolk antibodies in collected egg
yolk after three times of immunizationare extracted, purified and identified, and the
antibody titer is evaluated; the research on different yolk
powder preparation processes is carried out, the effect of lyophilized
powder preparation is significantly higher than that of
powder spraying technology, the matchingrelationship between the yolk and the
dilution, the lyophilization
temperature curve and other parameter conditions in the process are prepared, the cost is relatively low, the preparation efficiency is high,
antibody loss rate is only 10%, high
antibody titer,
antibody titer after high-temperature powder spraying shows a high downward trend, and the method is obviously superior to the high-temperature powder spraying technology for preparing yolk powder.