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Preparation method of micro-encapsulation coated yolk derived cholecystokinin antibodies

A technology for cholecystokinin and yolk, which is applied in the field of preparation of cholecystokinin antibodies derived from microcapsule-coated yolk, can solve the problems of carcass quality, average daily gain and feed intake not reaching a significant level, etc. Progress, oral administration, toxic side effects, strong operability effects

Inactive Publication Date: 2013-12-04
XINJIANG AGRI UNIV
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Problems solved by technology

Trout et al. actively immunized rams with CCK-8 to study its effect on ram carcasses and feed intake. The results showed that CCK-8 active immunized rams could produce higher antibodies and reduce the fat content of animal carcasses. However, the differences in carcass quality, average daily gain and feed intake did not reach a significant level

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  • Preparation method of micro-encapsulation coated yolk derived cholecystokinin antibodies
  • Preparation method of micro-encapsulation coated yolk derived cholecystokinin antibodies
  • Preparation method of micro-encapsulation coated yolk derived cholecystokinin antibodies

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Embodiment

[0022] 1) Preparation of cholecystokinin antigen complex: Dissolve 0.5mg CCK-8 and 6.5mg bovine serum albumin in 20ml 0.1M PBS, pH 7.0, add 5ml glutaraldehyde dropwise, stir at room temperature for 4h, in 4 Use a dialysis bag of 8000-14000Da in 0.005M PBS at pH 7.0 to fully dialyze overnight at ℃; the above mixture needs to be dialyzed for 3 days, and the solution is changed every 8 hours; take 10ml of CCK-BSA cross-linked complex and add an equal amount of Freund's complete adjuvant is emulsified, and after emulsification, it is formulated as a water-in-oil oil-emulsion vaccine containing antigen complexes.

[0023] 2) Preparation of yolk-derived cholecystokinin antibody: Dissolve 0.5mg CCK-8 and 6.5mg bovine serum albumin in 20ml 0.1M PBS (pH7.0), add 5ml glutaraldehyde dropwise, stir gently at room temperature for 4h, in Sufficient dialysis was carried out in 0.005M PBS at pH 7.0 with a 8000-14000Da dialysis bag overnight at a temperature of 4°C. The above mixture was dialy...

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Abstract

The invention provides a preparation method of micro-encapsulation coated yolk source cholecystokinin (CCK) antibodies. The preparation method comprises the following steps: preparing an antigens complex through adding freund's complete adjuvant in the mixture formed after a sheep derived CCK and BSA (Bovine Serum Albumin) are crosslinked to immunize laying hens, collecting eggs and obtaining the yolk derived CCK antibodies from the eggs, taking sodium alginate aqueous solution to perform the homogeneous emulsification with yolk derived CCK antibody liquid, dripping the emulsified solution into encystation solution consisting of chitosan and CaCl2 to be coated. The coated yolk derived cholecystokinin antibodies protect the effective actives of the yolk derived cholecystokinin antibodies, and have enteric solubility. According to the preparation method, a micro-encapsulation technology is adopted, and chitosan and sodium alginate are used as the coating materials which are natural polysaccharide, are low in cost, are taken orally and have no any toxic and side effect.

Description

technical field [0001] The invention relates to the development of veterinary biological products, and prepares a growth-promoting feed additive for young ruminants coated with microcapsules. Background technique [0002] Cholecystokinin (CCK) is an important polypeptide hormone in the gastrointestinal tract. The main function of CCK in the gastrointestinal tract is to promote gallbladder contraction and pancreatic enzyme secretion, and promote the growth of pancreatic cells. At the same time, it is considered to be an important endogenous Sexual physiological satiety factor is involved in food intake and body weight regulation. Through immune neutralization technology, it can partially eliminate the satiety effect caused by endogenous CCK, promote food intake, and thus promote animal growth. Yolk antibody (IgY) is a biological product that has attracted much attention at present, and is widely used in the prevention, diagnosis and treatment of human and animal diseases. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A61K39/395A61K9/50A61K47/36A61P3/00A61K38/02
Inventor 姚刚马蕾王金泉苏艳张晓红苏战强
Owner XINJIANG AGRI UNIV
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